LOCUS       sequence-218008-        3595 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ARRET with approximately 130 telomere hexanucleotide repeats of 
            TTAGGG and TTGGGG variant sequences under control of hH1 promoter 
            and terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3595)
  TITLE     telomeric repeat-containing RNA (TERRA and ARRET) plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pENTR hH1-ARRET
FEATURES             Location/Qualifiers
     source          1..3595
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        135..225
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     146..165
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(1152..1251)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1268..1287)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1269..1287)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1292..1308)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1292..1308)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1410..1429)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1421..2230
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1488..1507)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2377..2965
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2866..2885
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3119..3136
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3295..3322)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3414..3500)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3549..3571
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3563..3580
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3564..3580
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ttaaaggaac caattcagtc
      121 gatcgactgg atccatattt gcatgtcgct atgtgttctg ggaaatcacc ataaacgtga
      181 aatgtctttg gatttgggaa tcttataagt tctgtatgag accactcgcg gccagtggaa
      241 ttgtcgatct gatatcatcg atgaattccg aaccctaacc ctaaccctaa ccctaaccct
      301 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      361 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      421 aaccctaacc ccaaccccaa ccccaacccc aaccccaacc ccaaccccac ccccaacccc
      481 aaccccaacc ccaaccccaa ccccaacccc aaccccaacc ccaaccccaa ccccaacccc
      541 aaccccaacc ctaaccccaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      601 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      661 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      721 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ccaaccccaa ccctaaccct
      781 aaccctaacc ctaaccctaa ccctaaccct aaccccaacc ctaaccctaa ccctaaccct
      841 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      901 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaacccc
      961 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ccaaccctaa ccctaaccct
     1021 aacgctaacc ctaaccccaa ccctaacccc aaccccaacc ccaaccccaa ccccaaccgg
     1081 aattccgcgg aattcgagct cggtacccgg ggatcgacaa ttccactttt tttaattctc
     1141 gagatatcta gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     1201 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     1261 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     1321 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     1381 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     1441 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     1501 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     1561 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     1621 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     1681 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     1741 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     1801 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     1861 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     1921 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     1981 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     2041 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     2101 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     2161 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     2221 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     2281 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     2341 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2401 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2461 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2521 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2581 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2641 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2701 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2761 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2821 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2881 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2941 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3001 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3061 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3121 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     3181 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     3241 gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg
     3301 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct
     3361 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc
     3421 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac
     3481 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg
     3541 gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
