LOCUS       sequence-218010-        2916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knock down of hTERT transcript.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Kreilmeier T, Mejri D, Hauck M, Kleiter M, Holzmann K
  TITLE     Telomere Transcripts Target Telomerase in Human Cancer Cells.
  JOURNAL   Genes (Basel). 2016 Aug 16;7(8). pii: genes7080046. doi: 
            10.3390/genes7080046.
  PUBMED    27537914
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2016-08-16"; volume: "7(8). pii: genes7080046. 
            doi"; pages: " 10.3390/genes7080046"
COMMENT     Sequence Label: pENTR U6-hTERTsh
FEATURES             Location/Qualifiers
     source          1..2916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    complement(322..421)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(438..457)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(439..457)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(462..478)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(462..478)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(580..599)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             591..1400
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(658..677)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1547..2135
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2036..2055
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2289..2306
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2465..2492)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2584..2670)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2719..2741
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2733..2750
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2734..2750
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2766..2865
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg tttcatcagc aagtttggat tcaagagatc caaacttgct gatgaaattt
      301 ttgaattctc gagatatcta gacccagctt tcttgtacaa agttggcatt ataagaaagc
      361 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
      421 gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
      481 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
      541 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
      601 ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
      661 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga
      721 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
      781 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
      841 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag
      901 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
      961 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
     1021 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
     1081 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt
     1141 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     1201 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     1261 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     1321 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
     1381 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta
     1441 cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt
     1501 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1561 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1621 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1681 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1741 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1801 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1861 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     1921 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     1981 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2041 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2101 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2161 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2221 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2281 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     2341 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     2401 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     2461 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     2521 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     2581 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     2641 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa
     2701 cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg
     2761 ggccccaaat aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg
     2821 agcaatgctt ttttataatg ccaactttgt acaaaaaagc aggctttaaa ggaaccaatt
     2881 cagtcgactg gatctgtcga caaggtcggg caggaa
//
