LOCUS       sequence-218014-        9256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-tagged PC2 for Eukaryotic expression (883).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Doerr N, Wang Y, Kipp KR, Liu G, Benza JJ, Pletnev V, Pavlov TS, 
            Staruschenko A, Mohieldin AM, Takahashi M, Nauli SM, Weimbs T
  TITLE     Regulation of Polycystin-1 Function by Calmodulin Binding.
  JOURNAL   PLoS One. 2016 Aug 25;11(8):e0161525. doi: 
            10.1371/journal.pone.0161525. eCollection 2016.
  PUBMED    27560828
REFERENCE   2  (bases 1 to 9256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-08-25"; volume: "11(8):e0161525. doi"; pages: " 
            10.1371/journal.pone.0161525. eCollection 2016"
COMMENT     Sequence Label: pcDNA3 GFP-h-PKD2
FEATURES             Location/Qualifiers
     source          1..9256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      921..930
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(927..929,930..932,933..1643)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(927..929,930..932,933..1643)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(972..993)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1233..1252)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1580..1601
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(4808..4826)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4809..4826)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4846..4863)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4852..5076
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5122..5550
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5209..5228)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5419..5440
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5559..5579)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5564..5894
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5745..5880
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5961..6755
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6015..6034)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6625..6644
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6929..7050
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6966..6985)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7020..7039
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7099..7115)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7099..7115)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7112..7134)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7123..7139
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7147..7153,7154..7171,7172..7177))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7192..7213
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7330..7347)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7501..8089)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7581..7600)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8260..9051,9052..9120))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8883..8902
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9121..9225)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
      961 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     1021 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     1081 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     1141 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     1201 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     1261 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     1321 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     1381 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     1441 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     1501 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     1561 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1621 tcggcatgga cgagctgtac aagtctgcag cgccagtgac cgcgatggtg aactccagtc
     1681 gcgtgcagcc tcagcagccc ggggacgcca agcggccgcc cgcgccccgc gcgccggacc
     1741 cgggccggct gatggctggc tgcgcggccg tgggcgccag cctcgccgcc ccgggcggcc
     1801 tctgcgagca gcggggcctg gagatcgaga tgcagcgcat ccggcaggcg gccgcgcggg
     1861 accccccggc cggagccgcg gcctcccctt ctcctccgct ctcgtcgtgc tcccggcagg
     1921 cgtggagccg cgataacccc ggcttcgagg ccgaggagga ggaggaggag gtggaagggg
     1981 aagaaggcgg aatggtggtg gagatggacg tagagtggcg cccgggcagc cggaggtcgg
     2041 ccgcctcctc ggccgtgagc tccgtgggcg cgcggagccg ggggcttggg ggctaccacg
     2101 gcgcgggcca cccgagcggg aggcggcgcc ggcgagagga ccagggcccg ccgtgcccca
     2161 gcccagtcgg cggcggggac ccgctgcatc gccacctccc cctggaaggg cagccgcccc
     2221 gagtggcctg ggcggagagg ctggttcgcg ggctgcgagg tctctgggga acaagactca
     2281 tggaggaaag cagcactaac cgagagaaat accttaaaag tgttttacgg gaactggtca
     2341 catacctcct ttttctcata gtcttgtgca tcttgaccta cggcatgatg agctccaatg
     2401 tgtactacta cacccggatg atgtcacagc tcttcctaga cacccccgtg tccaaaacgg
     2461 agaaaactaa ctttaaaact ctgtcttcca tggaagactt ctggaagttc acagaaggct
     2521 ccttattgga tgggctgtac tggaagatgc agcccagcaa ccagactgaa gctgacaacc
     2581 gaagtttcat cttctatgag aacctgctgt taggggttcc acgaatacgg caactccgag
     2641 tcagaaatgg atcctgctct atcccccagg acttgagaga tgaaattaaa gagtgctatg
     2701 atgtctactc tgtcagtagt gaagataggg ctccctttgg gccccgaaat ggaaccgctt
     2761 ggatctacac aagtgaaaaa gacttgaatg gtagtagcca ctggggaatc attgcaactt
     2821 atagtggagc tggctattat ctggatttgt caagaacaag agaggaaaca gctgcacaag
     2881 ttgctagcct caagaaaaat gtctggctgg accgaggaac cagggcaact tttattgact
     2941 tctcagtgta caacgccaac attaacctgt tctgtgtggt caggttattg gttgaattcc
     3001 cagcaacagg tggtgtgatt ccatcttggc aatttcagcc tttaaagctg atccgatatg
     3061 tcacaacttt tgatttcttc ctggcagcct gtgagattat cttttgtttc tttatctttt
     3121 actatgtggt ggaagagata ttggaaattc gcattcacaa actacactat ttcaggagtt
     3181 tctggaattg tctggatgtt gtgatcgttg tgctgtcagt ggtagctata ggaattaaca
     3241 tatacagaac atcaaatgtg gaggtgctac tacagtttct ggaagatcaa aatactttcc
     3301 ccaactttga gcatctggca tattggcaga tacagttcaa caatatagct gctgtcacag
     3361 tattttttgt ctggattaag ctcttcaaat tcatcaattt taacaggacc atgagccagc
     3421 tctcgacaac catgtctcga tgtgccaaag acctgtttgg ctttgctatt atgttcttca
     3481 ttattttcct agcgtatgct cagttggcat accttgtctt tggcactcag gtcgatgact
     3541 tcagtacttt ccaagagtgt atcttcactc aattccgtat cattttgggc gatatcaact
     3601 ttgcagagat tgaggaagct aatcgagttt tgggaccaat ttatttcact acatttgtgt
     3661 tctttatgtt cttcattctt ttgaatatgt ttttggctat catcaatgat acttactctg
     3721 aagtgaaatc tgacttggca cagcagaaag ctgaaatgga actctcagat cttatcagaa
     3781 agggctacca taaagctttg gtcaaactaa aactgaaaaa aaataccgtg gatgacattt
     3841 cagagagtct gcggcaagga ggaggcaagt taaactttga cgaacttcga caagatctca
     3901 aagggaaggg ccatactgat gcagagattg aggcaatatt cacaaagtac gaccaagatg
     3961 gagaccaaga actgaccgaa catgaacatc agcagatgag agacgacttg gagaaagaga
     4021 gggaggacct ggatttggat cacagttctt taccacgtcc catgagcagc cgaagtttcc
     4081 ctcgaagcct ggatgactct gaggaggatg acgatgaaga tagcggacat agctccagaa
     4141 ggaggggaag catttctagt ggcgtttctt acgaagagtt tcaagtcctg gtgagacgag
     4201 tggaccggat ggagcattcc atcggcagca tagtgtccaa gattgacgcc gtgatcgtga
     4261 agctagagat tatggagcga gccaaactga agaggaggga ggtgctggga aggctgttgg
     4321 atggggtggc cgaggatgaa aggctgggtc gtgacagtga aatccatagg gaacagatgg
     4381 aacggctagt acgtgaagag ttggaacgct gggaatccga tgatgcagct tcccagatca
     4441 gtcatggttt aggcacgcca gtgggactaa atggtcaacc tcgccccaga agctcccgcc
     4501 catcttcctc ccaatctaca gaaggcatgg aaggtgcagg tggaaatggg agttctaatg
     4561 tccacgtatg atatgtgtgt ttcagtatgt gtgtttctaa taagtgagga agtggctgtc
     4621 ctgaattgct gtaacaagca cactatttat atgccctgac caccatagga tgctagtctt
     4681 tgtgaccgat tgctaatctt ctgcacttta atttatttta tataaacttt acccatggtt
     4741 caaaaaaaaa aaaaaaaaaa aaaaaaaaaa agggcggccg ctcgagcatg catctagagg
     4801 gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt
     4861 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     4921 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     4981 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     5041 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     5101 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     5161 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     5221 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca
     5281 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     5341 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     5401 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     5461 cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg
     5521 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     5581 tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt
     5641 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5701 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     5761 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5821 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5881 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
     5941 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     6001 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     6061 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     6121 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     6181 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     6241 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     6301 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     6361 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     6421 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     6481 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     6541 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     6601 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     6661 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     6721 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     6781 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     6841 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     6901 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     6961 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7021 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     7081 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7141 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7201 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7261 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7321 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7381 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7441 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7501 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7561 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7621 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7681 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7741 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7801 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7861 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7921 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     7981 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8041 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8101 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8161 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8221 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8281 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8341 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8401 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8461 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8521 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8581 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8641 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8701 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8761 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8821 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8881 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8941 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9001 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9061 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9121 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9181 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9241 agtgccacct gacgtc
//
