LOCUS       sequence-21802-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Tournier C, Whitmarsh AJ, Cavanagh J, Barrett T, Davis RJ
  TITLE     Mitogen-activated protein kinase kinase 7 is an activator of the 
            c-Jun NH2-terminal kinase.
  JOURNAL   Proc Natl Acad Sci U S A. 1997 Jul 8. 94(14):7337-42.
  PUBMED    9207092
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1997 Jul 8. 94(14):7337-42."
COMMENT     Sequence Label: pCDNA3 Flag MKK7a1
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..119
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt cggcaggcca acatggacta taaggacgat gatgacaaaa
      121 tgctggggct cccatcaacc ttgttcacac cgcgcagtat ggagagcatc gagattgacc
      181 agaagctgca ggagatcatg aagcagacag ggtacctgac tatcgggggc cagcgttatc
      241 aggcagaaat caatgacttg gagaacttgg gtgagatggg cagtggtacc tgtggtcagg
      301 tgtggaagat gcggttccgg aagacaggcc acatcattgc tgttaagcaa atgcggcgct
      361 ctgggaacaa ggaagagaat aagcgcattt tgatggacct ggatgtagta ctcaagagcc
      421 atgactgccc ttacatcgtt cagtgctttg gcaccttcat caccaacaca gacgtcttta
      481 ttgccatgga gctcatgggc acatgtgcag agaagctgaa gaaacgaatg cagggcccca
      541 ttccagagcg aatcctgggc aagatgactg tggcgattgt gaaagcactg tactatctga
      601 aggagaagca tggcgtcatc catcgcgatg tcaaaccctc caacatcctg ctagatgagc
      661 ggggccagat caagctctgt gactttggca tcagtggccg ccttgttgac tccaagccaa
      721 aacacggagt gctggctgtg ctgcctatat ggctcccg
//
