LOCUS       sequence-218024-        5621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus to aa168 of Flp recombinase fused to nMag CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2669_pCAG-PV1-FlpO-N168-L1-nMag-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..5621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1673..1693
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1703..1927
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2167..2186)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2232..2287
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2286..2305)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2648..2664)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2648..2664)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2661..2683)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2672..2688
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2696..2702,2703..2720,2721..2726))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2741..2762
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      2847..2981
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3001..3135
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3038..3057)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3092..3111
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3203..3220)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3374..3962)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3454..3473)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4133..4924,4925..4993))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4756..4775
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4994..5098)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5129..5508
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatga gccagttcga catcctgtgc aagacccccc ccaaggtgct
      721 ggtgcggcag ttcgtggaga gattcgagag gcccagcggc gagaagatcg ccagctgtgc
      781 cgccgagctg acctacctgt gctggatgat cacccacaac ggcaccgcca tcaagagggc
      841 caccttcatg agctacaaca ccatcatcag caacagcctg agcttcgaca tcgtgaacaa
      901 gagcctgcag ttcaagtaca agacccagaa ggccaccatc ctggaggcca gcctgaagaa
      961 gctgatcccc gcctgggagt tcaccatcat cccttacaac ggccagaagc accagagcga
     1021 catcaccgac atcgtgtcca gcctgcagct gcagttcgag agcagcgagg aggccgacaa
     1081 gggcaacagc cacagcaaga agatgctgaa ggccctgctg tccgagggcg agagcatctg
     1141 ggagatcacc gagaagatcc tgaacagctt cgagtacacc tccggagggt ctggctccgg
     1201 atcaagtggt ggcagcggta cccatactct ttatgccccc ggtggatatg acattatggg
     1261 atatctggac cagatcggca accggccaaa cccgcaggtg gaactgggcc ccgtggatac
     1321 atcctgcgcc ttgattcttt gtgacctgaa acagaaagac accccgatag tttacgcgag
     1381 tgaagccttc ctctacatga caggttacag caacgcagag gtgctgggcc ggaattgccg
     1441 gtttctgcaa agccctgacg gcatggtgaa gcccaagagc acccggaagt acgtggatag
     1501 taacacaatc aatactatgc gcaaggcaat cgacaggaat gccgaggtgc aggttgaagt
     1561 agtcaatttt aaaaagaatg gacagcgatt tgttaatttc ctgactatga tacctgttag
     1621 ggacgaaaca ggcgagtatc gatactctat gggattccag tgcgaaacag aacccaagaa
     1681 aaagcggaag gtgtgagaat tcctgtgcct tctagttgcc agccatctgt tgtttgcccc
     1741 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     1801 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     1861 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     1921 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     1981 tgtagcgtgt accgctggga gttcgtagac ggaaacaaac gcagaatcca agcgcggccg
     2041 cccaggatac atagattacc acaactccga gcccttccac cgctagcgca ctcctcaggt
     2101 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca
     2161 ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     2221 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     2281 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     2341 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata
     2401 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     2461 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     2521 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     2581 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt
     2641 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     2701 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     2761 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc
     2821 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccagtt ccgcccattc
     2881 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     2941 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     3001 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     3061 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     3121 tatcatgtct ggatccgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3181 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3241 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3301 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3361 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3421 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3481 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3541 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3601 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3661 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3721 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3781 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     3841 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3901 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3961 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4021 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4081 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4141 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4201 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4261 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4321 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4381 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4441 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4501 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4561 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4621 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4681 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4741 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4801 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4861 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4921 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     4981 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5041 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5101 catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta ttaatagtaa
     5161 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     5221 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     5281 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     5341 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     5401 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     5461 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc
     5521 cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta
     5581 tttatttttt aattattttg tgcagcgatg ggggcggggg g
//
