LOCUS       sequence-218039-        6554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of SFXN1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Kory N, Wyant GA, Prakash G, Uit de Bos J, Bottanelli F, Pacold ME, 
            Chan SH, Lewis CA, Wang T, Keys HR, Guo YE, Sabatini DM
  TITLE     SFXN1 is a mitochondrial serine transporter required for one-carbon 
            metabolism.
  JOURNAL   Science. 2018 Nov 16;362(6416). pii: 362/6416/eaat9528. doi: 
            10.1126/science.aat9528.
  PUBMED    30442778
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2018-11-16"; volume: "362(6416). pii: 362/6416/eaat9528. 
            doi"; pages: " 10.1126/science.aat9528"
COMMENT     Sequence Label: pMXs_SFXN1
FEATURES             Location/Qualifiers
     source          1..6554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             58..651
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    714..1071
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1130..1546
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1476..1498
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1514..1530
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1556..1930
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1868..1884
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     misc_feature    3053..3515
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3109..3126)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3336..3355
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3529..3924
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4095..4564
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4694..4711)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4865..5453)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4945..4964)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5617..6408,6409..6477))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6240..6259
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(6478..6554,1..28))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgcataat
       61 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
      121 gaaaaataca taactgagaa tagaaaagtt cagatcaagg tcaggaacag atggaacagc
      181 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
      241 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
      301 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga
      361 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
      421 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
      481 aaagagccca caacccctca ctcggcgcgc cagtcctccg attgactgag tcgcccgggt
      541 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
      601 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc
      661 gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg
      721 ccagcaactt atctgtgtct gtccgattgt ctagtgtcta tgactgattt tatgcgcctg
      781 cgtcggtact agttagctaa ctagctctgt atctggcgga cccgtggtgg aactgacgag
      841 ttcggaacac ccggccgcaa ccctgggaga cgtcccaggg acttcggggg ccgtttttgt
      901 ggcccgacct gagtccaaaa atcccgatcg ttttggactc tttggtgcac ccccctaata
      961 ggagggatat gtggttctgg taggagacga gaacctaaaa cagttcccgc ctccgtctga
     1021 atttttgctt tcggtttggg accgaagccg cgccgcgcgt cttgtctgct gcagcatcgt
     1081 tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt
     1141 taccactccc ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa
     1201 ccagtcggta gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac
     1261 ctttaacgtc ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa
     1321 gatcaaggtc ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac
     1381 ctgggaagcc ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc
     1441 tccgcctcct cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc
     1501 gcctcgatcc tccctttatc cagccctcac tccttctcta ggcgccccca tatggccata
     1561 tgagatctta tatggggcac ccccgcccct tgtaaacttc cctgaccctg acatgacaag
     1621 agttactaac agcccctctc tccaagctca cttacaggct ctctacttag tccagcacga
     1681 agtctggaga cctctggcgg cagcctacca agaacaactg gaccgaccgg tggtacctca
     1741 cccttaccga gtcggcgaca cagtgtgggt ccgccgacac cagactaaga acctagaacc
     1801 tcgctggaaa ggaccttaca cagtcctgct gaccaccccc accgccctca aagtagacgg
     1861 catcgcagct tggatacacg ccgcccacgt gaaggctgcc gaccccgggg gtggaccatc
     1921 ctctagactg ccggatctag ctagttaatt aaggatccgc caccatgagc ggtgaactgc
     1981 ctccaaacat aaatattaag gaaccacgat gggatcaatc tacgttcatc ggaagggcta
     2041 accatttctt tacagtgaca gacccgagga atattttgtt gaccaacgag caattggagt
     2101 ctgcaaggaa aatcgtccat gattaccgac aaggtattgt gccgccagga cttactgaaa
     2161 acgaactttg gagagcgaaa tacatttatg actctgcatt ccatccagac actggagaaa
     2221 agatgatcct gattggtcgc atgtcagctc aagtccccat gaatatgact ataactggct
     2281 gtatgatgac attttacaga acaacacccg ctgttctctt ttggcaatgg ataaatcaga
     2341 gcttcaacgc cgtagtaaac tacacaaaca gatccggcga cgctccactg actgtcaacg
     2401 aactcggaac tgcctacgtg tccgccacga ctggggccgt cgccaccgcc ctgggactta
     2461 atgcactgac gaagcatgtt agccctctca taggccggtt cgttccgttc gcggccgtag
     2521 cagccgctaa ctgtatcaac atacctctga tgagacagag ggaactgaag gtcggaatcc
     2581 cagtgaccga tgagaatggc aatcgactgg gcgagagtgc aaacgcagct aaacaggcga
     2641 taacgcaagt ggtagttagt aggatattga tggcggctcc cggaatggcg atacccccct
     2701 tcattatgaa caccctggag aaaaaggcgt ttttgaaacg cttcccatgg atgtctgcgc
     2761 caatacaggt cgggctcgta ggtttctgtt tggtctttgc tactcctctg tgttgcgcac
     2821 tcttccccca aaaaagcagt atgtcagtta caagcctcga ggctgagctc caagctaaaa
     2881 ttcaggagtc tcaccctgaa ttgcgacgag tttactttaa taagggactt taagcggccg
     2941 caggaggcgg tggaggcggt gcaggcgact acaaagacca tgackgkrat wakrmmgrtg
     3001 ntgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     3061 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     3121 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     3181 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     3241 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     3301 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     3361 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     3421 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     3481 gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat ggttcctttg
     3541 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc
     3601 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt
     3661 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct
     3721 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc
     3781 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa
     3841 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc
     3901 tctggttatg tgtgggaggg ctaagtcgac gataaaataa aagattttat ttagtctcca
     3961 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
     4021 attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg
     4081 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     4141 cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
     4201 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc
     4261 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
     4321 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
     4381 tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga
     4441 ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
     4501 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
     4561 ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat
     4621 ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     4681 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4741 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4801 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4861 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4921 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4981 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5041 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5101 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5161 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5221 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5281 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5341 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5401 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5461 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5521 tggtcatgag attatcaaaa aggatcttca cctagatcct tttgcggccg gccgcaaatc
     5581 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5641 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5701 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5761 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5821 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     5881 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     5941 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6001 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6061 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6121 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6181 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6241 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6301 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6361 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6421 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6481 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6541 atttgaatgt attt
//
