LOCUS       sequence-218043-        6390 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus to aa269 of VCre recombinase fused to ABI CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 6390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2585_pCAG-PV1-VCre-N269-L1-ABI-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..6390
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2417..2440
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2441..2461
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2471..2695
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2935..2954)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3000..3055
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3054..3073)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3416..3432)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3416..3432)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3429..3451)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3440..3456
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3464..3470,3471..3488,3489..3494))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3509..3530
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3615..3749
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3769..3903
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3806..3825)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3860..3879
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3971..3988)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4142..4730)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4222..4241)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4901..5692,5693..5761))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5524..5543
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5762..5866)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5897..6276
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatga tcgagaacca gctgagcctg ctgggcgact tttctggcgt
      721 gcggcccgac gatgtgaaaa ccgccattca ggccgcccag aaaaagggca tcaacgtggc
      781 cgagaacgag cagttcaagg ccgccttcga gcatctgctg aacgagttca agaagcggga
      841 agagagatac agccccaaca ccctgcggcg gctggaaagc gcctggacct gcttcgtgga
      901 ttggtgcctg gccaaccaca gacacagcct gcctgccacc cccgataccg tggaagcctt
      961 cttcatcgag cgggccgagg aactgcaccg gaacaccctg agcgtgtaca gatgggccat
     1021 cagccgggtg cacagagtgg ccggatgccc tgatccctgc ctggacatct acgtggaaga
     1081 tcggctgaag gccattgccc ggaagaaagt gcgggaaggc gaggccgtga agcaggccag
     1141 ccctttcaac gagcagcatc tgctgaagct gaccagcctg tggtacagaa gcgacaagct
     1201 gctgctgcgg cggaacctgg ctctgctggc tgtggcctac gagagcatgc tgagagccag
     1261 cgagctggcc aacatccggg tgtccgatat ggaactggcc ggcgacggaa ccgccatcct
     1321 gaccatccct atcaccaaga ccaaccactc cggcgagccc gatacctgca tcctgtccca
     1381 ggatgtggtg tccctgctga tggactacac cgaggccggc aagctggata tgagcagcga
     1441 cggcttcctg ttcgtgggcg tgtccaagca caacacctgt atctccggag ggtctggctc
     1501 cggatcaagt ggtggcagcg gtaccccttt gtatggtttt acttcgattt gtggaagaag
     1561 acctgagatg gaagatgctg tttcgactat accaagattc cttcaatctt cctctggttc
     1621 gatgttagat ggtcggtttg atcctcaatc cgccgctcat ttcttcggtg tttacgacgg
     1681 ccatggcggt tctcaggtag cgaactattg tagagagagg atgcatttgg ctttggcgga
     1741 ggagatagct aaggagaaac cgatgctctg cgatggtgat acgtggctgg agaagtggaa
     1801 gaaagctctt ttcaactcgt tcctgagagt tgactcggag attgggtcag ttgcgccgga
     1861 gacggttggg tcaacgtcgg tggttgccgt tgttttcccg tctcacatct tcgtcgctaa
     1921 ctgcggtgac tctagagccg ttctttgccg cggcaaaact gcacttccat tatccgttga
     1981 ccataaaccg gatagagaag atgaagctgc gaggattgaa gccgcaggag ggaaagtgat
     2041 tcagtggaat ggagctcgtg ttttcggtgt tctcgccatg tcgagatcca ttggcgatag
     2101 atacttgaaa ccatccatca ttcctgatcc ggaagtgacg gctgtgaaga gagtaaaaga
     2161 agatgattgt ctgattttgg cgagtgacgg ggtttgggat gtaatgacgg atgaagaagc
     2221 gtgtgagatg gcaaggaagc ggattctctt gtggcacaag aaaaacgcgg tggctgggga
     2281 tgcatcgttg ctcgcggatg agcggagaaa ggaagggaaa gatcctgcgg cgatgtccgc
     2341 ggctgagtat ttgtcaaagc tggcgataca gagaggaagc aaagacaaca taagtgtggt
     2401 ggtggttgat ttgaaggatt acaaggacga tgacgataag cccaagaaaa agcggaaggt
     2461 gtgagaattc ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     2521 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     2581 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     2641 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     2701 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcgtgtac
     2761 cgctgggagt tcgtagacgg aaacaaacgc agaatccaag cgcggccgcc caggatacat
     2821 agattaccac aactccgagc ccttccaccg ctagcgcact cctcaggtgc aggctgccta
     2881 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt
     2941 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta
     3001 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     3061 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt
     3121 ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca
     3181 gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg
     3241 ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc
     3301 cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt
     3361 ccctcttctc ttatggagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat
     3421 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     3481 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     3541 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta
     3601 gtcagcaacc atagtcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     3661 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     3721 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt
     3781 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3841 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     3901 atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     3961 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     4021 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4081 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4141 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4201 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4261 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4321 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4381 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4441 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     4501 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     4561 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     4621 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     4681 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     4741 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     4801 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     4861 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     4921 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     4981 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     5041 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     5101 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     5161 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     5221 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     5281 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     5341 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     5401 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     5461 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     5521 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     5581 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     5641 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     5701 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     5761 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     5821 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     5881 aagtgccacc tgggtcgaca ttgattattg actagttatt aatagtaatc aattacgggg
     5941 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     6001 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     6061 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     6121 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     6181 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     6241 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
     6301 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
     6361 ttattttgtg cagcgatggg ggcggggggg
//
