LOCUS       sequence-218045-        6414 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus to aa277of VCre recombinase fused to ABI CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6414)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 6414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW2619_pCAG-PV1-VCre-N277-L1-ABI-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..6414
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2441..2464
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2465..2485
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2495..2719
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2959..2978)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3024..3079
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3078..3097)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3440..3456)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3440..3456)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3453..3475)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3464..3480
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3488..3494,3495..3512,3513..3518))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3533..3554
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3639..3773
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3793..3927
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3830..3849)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3884..3903
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3995..4012)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4166..4754)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4246..4265)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4925..5716,5717..5785))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5548..5567
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5786..5890)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5921..6300
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatga tcgagaacca gctgagcctg ctgggcgact tttctggcgt
      721 gcggcccgac gatgtgaaaa ccgccattca ggccgcccag aaaaagggca tcaacgtggc
      781 cgagaacgag cagttcaagg ccgccttcga gcatctgctg aacgagttca agaagcggga
      841 agagagatac agccccaaca ccctgcggcg gctggaaagc gcctggacct gcttcgtgga
      901 ttggtgcctg gccaaccaca gacacagcct gcctgccacc cccgataccg tggaagcctt
      961 cttcatcgag cgggccgagg aactgcaccg gaacaccctg agcgtgtaca gatgggccat
     1021 cagccgggtg cacagagtgg ccggatgccc tgatccctgc ctggacatct acgtggaaga
     1081 tcggctgaag gccattgccc ggaagaaagt gcgggaaggc gaggccgtga agcaggccag
     1141 ccctttcaac gagcagcatc tgctgaagct gaccagcctg tggtacagaa gcgacaagct
     1201 gctgctgcgg cggaacctgg ctctgctggc tgtggcctac gagagcatgc tgagagccag
     1261 cgagctggcc aacatccggg tgtccgatat ggaactggcc ggcgacggaa ccgccatcct
     1321 gaccatccct atcaccaaga ccaaccactc cggcgagccc gatacctgca tcctgtccca
     1381 ggatgtggtg tccctgctga tggactacac cgaggccggc aagctggata tgagcagcga
     1441 cggcttcctg ttcgtgggcg tgtccaagca caacacctgt atcaagccca agaaggacaa
     1501 gcagacctcc ggagggtctg gctccggatc aagtggtggc agcggtaccc ctttgtatgg
     1561 ttttacttcg atttgtggaa gaagacctga gatggaagat gctgtttcga ctataccaag
     1621 attccttcaa tcttcctctg gttcgatgtt agatggtcgg tttgatcctc aatccgccgc
     1681 tcatttcttc ggtgtttacg acggccatgg cggttctcag gtagcgaact attgtagaga
     1741 gaggatgcat ttggctttgg cggaggagat agctaaggag aaaccgatgc tctgcgatgg
     1801 tgatacgtgg ctggagaagt ggaagaaagc tcttttcaac tcgttcctga gagttgactc
     1861 ggagattggg tcagttgcgc cggagacggt tgggtcaacg tcggtggttg ccgttgtttt
     1921 cccgtctcac atcttcgtcg ctaactgcgg tgactctaga gccgttcttt gccgcggcaa
     1981 aactgcactt ccattatccg ttgaccataa accggataga gaagatgaag ctgcgaggat
     2041 tgaagccgca ggagggaaag tgattcagtg gaatggagct cgtgttttcg gtgttctcgc
     2101 catgtcgaga tccattggcg atagatactt gaaaccatcc atcattcctg atccggaagt
     2161 gacggctgtg aagagagtaa aagaagatga ttgtctgatt ttggcgagtg acggggtttg
     2221 ggatgtaatg acggatgaag aagcgtgtga gatggcaagg aagcggattc tcttgtggca
     2281 caagaaaaac gcggtggctg gggatgcatc gttgctcgcg gatgagcgga gaaaggaagg
     2341 gaaagatcct gcggcgatgt ccgcggctga gtatttgtca aagctggcga tacagagagg
     2401 aagcaaagac aacataagtg tggtggtggt tgatttgaag gattacaagg acgatgacga
     2461 taagcccaag aaaaagcgga aggtgtgaga attcctgtgc cttctagttg ccagccatct
     2521 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     2581 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     2641 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     2701 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     2761 ccccacgcgc cctgtagcgt gtaccgctgg gagttcgtag acggaaacaa acgcagaatc
     2821 caagcgcggc cgcccaggat acatagatta ccacaactcc gagcccttcc accgctagcg
     2881 cactcctcag gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc
     2941 tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc
     3001 ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     3061 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca
     3121 gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa
     3181 aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt
     3241 ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt
     3301 ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc
     3361 ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc
     3421 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     3481 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     3541 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     3601 gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccag
     3661 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     3721 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     3781 ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     3841 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     3901 atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa cgcgcgggga
     3961 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     4021 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     4081 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4141 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4201 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4261 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4321 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4381 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4441 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4501 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4561 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     4621 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4681 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4741 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4801 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4861 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4921 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4981 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5041 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5101 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5161 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5221 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5281 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5341 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5401 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5461 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5521 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     5581 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5641 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5701 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5761 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     5821 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     5881 gggttccgcg cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagt
     5941 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     6001 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     6061 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     6121 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     6181 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     6241 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     6301 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
     6361 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggg
//
