LOCUS       sequence-21805-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Tournier C, Whitmarsh AJ, Cavanagh J, Barrett T, Davis RJ
  TITLE     Mitogen-activated protein kinase kinase 7 is an activator of the 
            c-Jun NH2-terminal kinase.
  JOURNAL   Proc Natl Acad Sci U S A. 1997 Jul 8. 94(14):7337-42.
  PUBMED    9207092
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1997 Jul 8. 94(14):7337-42."
COMMENT     Sequence Label: pCDNA3 Flag MKK7a1 (K76A)
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt cggcagccaa catggactat aaggacgatg atgacaaaat
      121 gctggggctc ccatcaacct tgttcacacc gcgcagtatg gagagcatcg agattgacca
      181 gaagctgcag gagatcatga agcagacagg gtacctgact atcgggggcc agcgttatca
      241 ggcagaaatc aatgacttgg agaacttggg tgagatgggc agtggtacct gtggtcaggt
      301 gtggaagatg cggttccgga agacaggcca catcattgct gttgcgcaaa tgcggcgctc
      361 tgggaacaag gaagagaata agcgcatttt gatggacctg gatgtagtac tcaagagcca
      421 tgactgccct tacatcgttc agtgctttgg caccttcatc accaacacag acgtctttat
      481 tgccatggag ctcatgggca catgtgcaga gaagctgaag aaacgaatgc agggccccat
      541 tccagagcga atcctgggca agatgactgt ggcgattgtg aaagcactgt actatctgaa
      601 ggagaagcat ggcgtcatcc atcgcgatgt caaaccctcc aacatcctgc tagatgagcg
      661 gggccagatc aagctctgtg actttggcat cagtggccgc cttgttgact ccaaagccaa
      721 aacacggagg tgctggctgt gctgcctata tggc
//
