LOCUS       sequence-218058-        6853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus to aa428 of PhiC31 integrase fused to ABI CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 6853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW1602_pCAG-PV1-PhiC31-N428-L1-ABI-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..6853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      671..680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2894..2917
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2918..2938
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2948..3172
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3412..3431)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3477..3532
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3531..3550)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3893..3909)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3893..3909)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3906..3928)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3917..3933
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3941..3947,3948..3965,3966..3971))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3986..4007
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      4092..4226
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4246..4380
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4283..4302)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4337..4356
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4448..4465)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4619..5207)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4699..4718)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5378..6169,6170..6238))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6001..6020
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6239..6343)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6374..6753
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgg atacctacgc cggagcctac gacagacaga gccgggagag
      721 agagaacagc agcgccgcca gccccgccac ccagagaagc gccaacgagg ataaggccgc
      781 cgatctgcag agagaggtgg agagggacgg cggcagattc agatttgtgg gccacttcag
      841 cgaggcccct ggcaccagcg ccttcggcac cgccgagaga cccgagttcg agagaatcct
      901 gaacgagtgt agggccggca ggctgaacat gatcatcgtg tacgacgtgt cccggttcag
      961 caggctgaag gtgatggacg ccatccctat cgtgtccgag ctgctggccc tgggcgtgac
     1021 catcgtgtcc acccaggaag gcgtctttag acagggcaac gtgatggacc tgatccacct
     1081 gatcatgagg ctggacgcca gccacaagga gagcagcctg aagagcgcca agatcctgga
     1141 caccaagaac ctgcagaggg agctgggcgg ctatgtgggc ggcaaggccc cctacggctt
     1201 cgagctggtg tccgagacca aggagatcac ccggaacggc aggatggtga acgtggtgat
     1261 caacaagctg gcccacagca ccacccccct gaccggcccc ttcgagtttg agcccgacgt
     1321 gatcaggtgg tggtggcggg agatcaagac ccacaagcac ctgcctttca agcccggcag
     1381 ccaggccgcc atccaccccg gcagcatcac cggcctgtgt aagagaatgg acgccgacgc
     1441 cgtgcccacc agaggcgaga ccatcggcaa gaaaaccgcc agcagcgcct gggaccccgc
     1501 caccgtgatg agaatcctga gggaccctag gatcgccggc ttcgccgccg aggtgatcta
     1561 caagaagaag cccgacggca cccccaccac caagatcgag ggctacagaa tccagagaga
     1621 ccccatcacc ctgagacctg tggagctgga ctgtggccct atcatcgagc ctgccgagtg
     1681 gtacgagctg caggcctggc tggacggcag aggcagaggc aagggcctga gcagaggcca
     1741 ggccatcctg agcgccatgg acaagctgta ctgtgagtgt ggcgccgtga tgaccagcaa
     1801 gagaggcgag gagagcatca aggacagcta ccggtgccgg agaagaaagg tggtggaccc
     1861 cagcgcccct ggccagcacg agggcacctg taatgtgagc atggccgccc tggacaagtt
     1921 cgtggccgag cggatcttca acaagatccg gcacgccgag tccggagggt ctggctccgg
     1981 atcaagtggt ggcagcggta cccctttgta tggttttact tcgatttgtg gaagaagacc
     2041 tgagatggaa gatgctgttt cgactatacc aagattcctt caatcttcct ctggttcgat
     2101 gttagatggt cggtttgatc ctcaatccgc cgctcatttc ttcggtgttt acgacggcca
     2161 tggcggttct caggtagcga actattgtag agagaggatg catttggctt tggcggagga
     2221 gatagctaag gagaaaccga tgctctgcga tggtgatacg tggctggaga agtggaagaa
     2281 agctcttttc aactcgttcc tgagagttga ctcggagatt gggtcagttg cgccggagac
     2341 ggttgggtca acgtcggtgg ttgccgttgt tttcccgtct cacatcttcg tcgctaactg
     2401 cggtgactct agagccgttc tttgccgcgg caaaactgca cttccattat ccgttgacca
     2461 taaaccggat agagaagatg aagctgcgag gattgaagcc gcaggaggga aagtgattca
     2521 gtggaatgga gctcgtgttt tcggtgttct cgccatgtcg agatccattg gcgatagata
     2581 cttgaaacca tccatcattc ctgatccgga agtgacggct gtgaagagag taaaagaaga
     2641 tgattgtctg attttggcga gtgacggggt ttgggatgta atgacggatg aagaagcgtg
     2701 tgagatggca aggaagcgga ttctcttgtg gcacaagaaa aacgcggtgg ctggggatgc
     2761 atcgttgctc gcggatgagc ggagaaagga agggaaagat cctgcggcga tgtccgcggc
     2821 tgagtatttg tcaaagctgg cgatacagag aggaagcaaa gacaacataa gtgtggtggt
     2881 ggttgatttg aaggattaca aggacgatga cgataagccc aagaaaaagc ggaaggtgtg
     2941 agaattcctg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     3001 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     3061 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     3121 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     3181 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cgtgtaccgc
     3241 tgggagttcg tagacggaaa caaacgcaga atccaagcgc ggccgcccag gatacataga
     3301 ttaccacaac tccgagccct tccaccgcta gcgcactcct caggtgcagg ctgcctatca
     3361 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
     3421 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     3481 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     3541 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     3601 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
     3661 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     3721 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     3781 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     3841 tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc
     3901 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     3961 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     4021 cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc
     4081 agcaaccata gtcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     4141 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     4201 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca
     4261 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     4321 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     4381 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     4441 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     4501 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     4561 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4621 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4681 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     4741 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     4801 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     4861 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     4921 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     4981 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5041 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     5101 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     5161 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5221 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     5281 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     5341 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     5401 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     5461 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     5521 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     5581 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     5641 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     5701 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     5761 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     5821 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     5881 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     5941 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     6001 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6061 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6121 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     6181 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     6241 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     6301 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     6361 tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat tacggggtca
     6421 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     6481 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     6541 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     6601 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     6661 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     6721 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
     6781 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
     6841 ttttgtgcag cga
//
