LOCUS       sequence-218059-        3389 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Preparation of RNA probe for ISH of mouse Vmn2r76 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Akiyoshi S, Ishii T, Bai Z, Mombaerts P
  TITLE     Subpopulations of vomeronasal sensory neurons with coordinated 
            coexpression of type 2 vomeronasal receptor genes are differentially
            dependent on Vmn2r1.
  JOURNAL   Eur J Neurosci. 2018 Apr;47(7):887-900. doi: 10.1111/ejn.13875. Epub
            2018 Mar 25.
  PUBMED    29465786
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/ejn.13875";
            journalName: "Eur J Neurosci"; date: "2018-04"; volume: "47"; issue:
            "7"; pages: "887-900"
COMMENT     Sequence Label: pISH-Vmn2r76-VR2
FEATURES             Location/Qualifiers
     source          1..3389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             108..119
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(514..532)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(515..532)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(550..566)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(550..566)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(563..585)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    574..590
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(598..604,605..622,623..628))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    643..664
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(781..798)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(952..1540)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1032..1051)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1711..2502,2503..2571))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2334..2353
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2572..2676)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2754..3209)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2891..2912)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3103..3122
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3335..3357
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3349..3366
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3350..3366
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3373..3389,1..3)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(3373..3389,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tctcctgagg ggattttcta aatcctggat gacaaggtat actgcatatt gagggtagag
      121 tctgtctaac atctgtggcc cagtctacca attcctcata cataagcaat tcttgaggat
      181 gtgtaaagta tttgaaaaac tttcctattt tcaccttaat tccaaaactt tgtaaaaaat
      241 ttccagtgta gaaaatatca taatttatat tcatgttttc tttctgattc aagttcacta
      301 ggtctccagc aggatttaca aaatggatgt tcttcaggga agagagcacc tgcaaagaat
      361 caaactccag tcctttccca acattatttg acaatgtatt tgcttgctgc agaagtaact
      421 cattgagtga atgaatcact agtgaattcg cggccgcctg caggtcgacc atatgggaga
      481 gctcccaacg cgttggatgc atagcttgag tattctatag tgtcacctaa atagcttggc
      541 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      601 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      661 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      721 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
      781 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      841 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      901 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      961 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1021 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     1081 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     1141 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1201 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1261 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1321 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1381 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1441 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1501 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1561 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1621 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     1681 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     1741 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     1801 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     1861 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     1921 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     1981 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     2041 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     2101 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     2161 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     2221 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     2281 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     2341 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     2401 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     2461 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     2521 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     2581 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     2641 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     2701 tgatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt
     2761 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     2821 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     2881 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     2941 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     3001 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     3061 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     3121 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     3181 cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg
     3241 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     3301 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     3361 gccagtgaat tgtaatacga ctcactata
//
