LOCUS       sequence-21806-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Derijard B, Raingeaud J, Barrett T, Wu IH, Han J, Ulevitch RJ, Davis
            RJ
  TITLE     Independent human MAP-kinase signal transduction pathways defined by
            MEK and MKK isoforms.
  JOURNAL   Science. 1995 Feb 3. 267(5198):682-5.
  PUBMED    7839144
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            1995 Feb 3. 267(5198):682-5."
COMMENT     Sequence Label: pCDNA3 Flag MKK4
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             117..140
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttggtac cgagctcgga tccggcagcc aacatggact
      121 ataaggacga tgacgacaaa cagggtaaac gcaaagcact gaagttgaat tttgcaaatc
      181 cacctttcaa atctacagca aggtttactc tgaatcccaa tcctacagga gttcaaaacc
      241 cacacataga gagactgaga acacacagca ttgagtcatc aggaaaactg aagatctccc
      301 ctgaacaaca ctgggatttc actgcagagg acttgaaaga ccttggagaa attggacgag
      361 gagcttatgg ttctgtcaac aaaatggtcc acaaaccaag tgggcaaata atggcagtta
      421 aaagaattcg gtcaacagtg gatgaaaaag aacaaaaaca acttcttatg gatttggatg
      481 tagtaatgcg gagtagtgat tgcccataca ttgttcagtt ttatggtgca ctcttcagag
      541 agggtgactg ttggatctgt atggaactca tgtctacctc gtttgataag ttttacaaat
      601 atgtatatag tgtattagat gatgttattc cagaagaaat tttaggcaaa atcactttag
      661 caactgtgaa agcactaaac cacttaaaag aaaacttgaa aattattcac agagatatca
      721 aaccttccaa tattcttctg gacagaagtg gaaatattaa gctctgtgac ttcggcatca
      781 gtggacagct tgtggactct attgccaaga caagagatgc tggctgt
//
