LOCUS       sequence-218060-        7293 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus to aa571 of PhiC31 integrase fused to ABI CID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7293)
  AUTHORS   Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, 
            Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J,
            Wong WW
  TITLE     High-performance chemical- and light-inducible recombinases in 
            mammalian cells and mice.
  JOURNAL   Nat Commun. 2019 Oct 24;10(1):4845. doi: 10.1038/s41467-019-12800-7.
  PUBMED    31649244
REFERENCE   2  (bases 1 to 7293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-24"; volume: "10(1):4845. doi"; pages: " 
            10.1038/s41467-019-12800-7"
COMMENT     Sequence Label: pBW1603_pCAG-PV1-PhiC31-N571-L1-ABI-NLS-BGHpA
FEATURES             Location/Qualifiers
     source          1..7293
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     466..488
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     550..569
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      671..680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3323..3346
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3347..3367
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3377..3601
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(3841..3860)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3906..3961
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3960..3979)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4322..4338)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4322..4338)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4335..4357)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4346..4362
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4370..4376,4377..4394,4395..4400))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4415..4436
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      4521..4655
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4675..4809
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4712..4731)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4766..4785
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4877..4894)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5048..5636)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5128..5147)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5807..6598,6599..6667))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6430..6449
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6668..6772)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        6803..7182
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
       61 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
      121 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
      181 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
      241 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
      301 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
      361 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
      421 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
      481 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
      541 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattgatta
      601 attcgagcga aggcgcgccc attactcgca tccattctca ggctgtctcg tctcgtctcg
      661 cacgcgtgcc gccaccatgg atacctacgc cggagcctac gacagacaga gccgggagag
      721 agagaacagc agcgccgcca gccccgccac ccagagaagc gccaacgagg ataaggccgc
      781 cgatctgcag agagaggtgg agagggacgg cggcagattc agatttgtgg gccacttcag
      841 cgaggcccct ggcaccagcg ccttcggcac cgccgagaga cccgagttcg agagaatcct
      901 gaacgagtgt agggccggca ggctgaacat gatcatcgtg tacgacgtgt cccggttcag
      961 caggctgaag gtgatggacg ccatccctat cgtgtccgag ctgctggccc tgggcgtgac
     1021 catcgtgtcc acccaggaag gcgtctttag acagggcaac gtgatggacc tgatccacct
     1081 gatcatgagg ctggacgcca gccacaagga gagcagcctg aagagcgcca agatcctgga
     1141 caccaagaac ctgcagaggg agctgggcgg ctatgtgggc ggcaaggccc cctacggctt
     1201 cgagctggtg tccgagacca aggagatcac ccggaacggc aggatggtga acgtggtgat
     1261 caacaagctg gcccacagca ccacccccct gaccggcccc ttcgagtttg agcccgacgt
     1321 gatcaggtgg tggtggcggg agatcaagac ccacaagcac ctgcctttca agcccggcag
     1381 ccaggccgcc atccaccccg gcagcatcac cggcctgtgt aagagaatgg acgccgacgc
     1441 cgtgcccacc agaggcgaga ccatcggcaa gaaaaccgcc agcagcgcct gggaccccgc
     1501 caccgtgatg agaatcctga gggaccctag gatcgccggc ttcgccgccg aggtgatcta
     1561 caagaagaag cccgacggca cccccaccac caagatcgag ggctacagaa tccagagaga
     1621 ccccatcacc ctgagacctg tggagctgga ctgtggccct atcatcgagc ctgccgagtg
     1681 gtacgagctg caggcctggc tggacggcag aggcagaggc aagggcctga gcagaggcca
     1741 ggccatcctg agcgccatgg acaagctgta ctgtgagtgt ggcgccgtga tgaccagcaa
     1801 gagaggcgag gagagcatca aggacagcta ccggtgccgg agaagaaagg tggtggaccc
     1861 cagcgcccct ggccagcacg agggcacctg taatgtgagc atggccgccc tggacaagtt
     1921 cgtggccgag cggatcttca acaagatccg gcacgccgag ggcgacgagg agaccctggc
     1981 cctgctgtgg gaggccgcca gaagattcgg caagctgacc gaggcccccg agaagagcgg
     2041 cgagagggcc aacctggtgg ccgagagagc cgacgccctg aacgccctgg aggagctgta
     2101 cgaggacaga gccgccggag cctatgacgg ccctgtgggc aggaagcact tcagaaagca
     2161 gcaggccgcc ctgaccctga gacagcaggg cgccgaggaa agactggccg agctggaggc
     2221 cgccgaggcc cctaagctgc ccctggatca gtggttcccc gaggatgccg acgccgaccc
     2281 caccggcccc aagtcctggt ggggcagagc cagcgtggac gacaagaggg tgttcgtggg
     2341 cctgttcgtg gataagatcg tggtgaccaa gagcaccacc ggcaggggct ccggagggtc
     2401 tggctccgga tcaagtggtg gcagcggtac ccctttgtat ggttttactt cgatttgtgg
     2461 aagaagacct gagatggaag atgctgtttc gactatacca agattccttc aatcttcctc
     2521 tggttcgatg ttagatggtc ggtttgatcc tcaatccgcc gctcatttct tcggtgttta
     2581 cgacggccat ggcggttctc aggtagcgaa ctattgtaga gagaggatgc atttggcttt
     2641 ggcggaggag atagctaagg agaaaccgat gctctgcgat ggtgatacgt ggctggagaa
     2701 gtggaagaaa gctcttttca actcgttcct gagagttgac tcggagattg ggtcagttgc
     2761 gccggagacg gttgggtcaa cgtcggtggt tgccgttgtt ttcccgtctc acatcttcgt
     2821 cgctaactgc ggtgactcta gagccgttct ttgccgcggc aaaactgcac ttccattatc
     2881 cgttgaccat aaaccggata gagaagatga agctgcgagg attgaagccg caggagggaa
     2941 agtgattcag tggaatggag ctcgtgtttt cggtgttctc gccatgtcga gatccattgg
     3001 cgatagatac ttgaaaccat ccatcattcc tgatccggaa gtgacggctg tgaagagagt
     3061 aaaagaagat gattgtctga ttttggcgag tgacggggtt tgggatgtaa tgacggatga
     3121 agaagcgtgt gagatggcaa ggaagcggat tctcttgtgg cacaagaaaa acgcggtggc
     3181 tggggatgca tcgttgctcg cggatgagcg gagaaaggaa gggaaagatc ctgcggcgat
     3241 gtccgcggct gagtatttgt caaagctggc gatacagaga ggaagcaaag acaacataag
     3301 tgtggtggtg gttgatttga aggattacaa ggacgatgac gataagccca agaaaaagcg
     3361 gaaggtgtga gaattcctgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     3421 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     3481 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     3541 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     3601 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     3661 gtgtaccgct gggagttcgt agacggaaac aaacgcagaa tccaagcgcg gccgcccagg
     3721 atacatagat taccacaact ccgagccctt ccaccgctag cgcactcctc aggtgcaggc
     3781 tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga
     3841 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc
     3901 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     3961 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta
     4021 gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg
     4081 tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac
     4141 ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa
     4201 attttcctta catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat
     4261 agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg gcgtaatcat
     4321 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4381 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4441 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct
     4501 caattagtca gcaaccatag tcccgcccct aactccgccc agttccgccc attctccgcc
     4561 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     4621 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg
     4681 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4741 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     4801 gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     4861 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     4921 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     4981 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5041 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5101 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5161 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5221 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5281 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5341 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5401 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5461 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     5521 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5581 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5641 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     5701 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     5761 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     5821 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     5881 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     5941 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6001 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6061 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6121 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6181 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6241 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6301 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6361 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6421 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6481 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6541 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6601 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6661 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6721 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     6781 cccgaaaagt gccacctggg tcgacattga ttattgacta gttattaata gtaatcaatt
     6841 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     6901 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     6961 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     7021 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     7081 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     7141 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg
     7201 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt
     7261 ttttaattat tttgtgcagc gatgggggcg ggg
//
