LOCUS       sequence-218070-        3469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for T7 transcription of wt actin pre-mRNA (del6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Villa T, Guthrie C
  TITLE     The Isy1p component of the NineTeen complex interacts with the 
            ATPase Prp16p to regulate the fidelity of pre-mRNA splicing.
  JOURNAL   Genes Dev. 2005 Aug 15;19(16):1894-904. doi: 10.1101/gad.1336305.
  PUBMED    16103217
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2005-08-15- 15"; volume: "19"; 
            issue: "16"; pages: "1894-904"
COMMENT     Sequence Label: puC19 preACT1(WTdel6)
FEATURES             Location/Qualifiers
     source          1..3469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..31
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     23..40
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     24..40
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(657..673)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(705..723)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(744..760)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(744..760)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(757..779)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    768..784
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(792..798,799..816,817..822))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    837..858
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(975..992)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1146..1734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1226..1245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1905..2696,2697..2765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2528..2547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2766..2870)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2896..3351)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3033..3054)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3245..3264
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact
       61 atagggcgaa ttggagctcg ccccttttag atttttcacg cttactgctt ttttcttccc
      121 aagatcgaaa atttactgaa ttaacaatgg attctggtat gttctagcgc ttgcaccatc
      181 ccatttaact gtaagaagaa ttgcacggtc ccaattgctc gagagatttc tcttttacct
      241 ttttttacta tttttcactc tcccataacc tcctatattg actgatctgt aataaccacg
      301 atattattgg aataaatagg ggcttgaaat ttggaaaaaa aaaaaaactg aaatattttc
      361 gtgataagtg atagtgatat tcttctttta tttgctactg tgtctcatgt actaacatcg
      421 attgcttcat tctttttgtt gctatattat atgtttagag gttgctgctt tggttattga
      481 taacggttct ggtatgtgta aagccggttt tgccggtgac gacgctcctc gtgctgtctt
      541 cccatctatc gtcggtagac caagacacca aggtatcatg gtcggtatgg gtcaaaaaga
      601 ctcctacgtt ggtgatgaag gggatcctct agagtcgacc tgcagcccaa gcttatcgat
      661 accgtcgacc tcgtcgaggg ggggcccggt accagctttt gttcccttta gtgagggtta
      721 atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
      781 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
      841 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
      901 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
      961 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1021 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     1081 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1141 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1201 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1261 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1321 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1381 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1441 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1501 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1561 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1621 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1681 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1741 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1801 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1861 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1921 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1981 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2041 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     2101 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     2161 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     2221 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2281 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     2341 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     2401 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     2461 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     2521 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     2581 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     2641 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     2701 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     2761 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2821 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2881 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     2941 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     3001 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     3061 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     3121 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     3181 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     3241 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     3301 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
     3361 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     3421 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcc
//
