LOCUS       sequence-218072-        3318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Villa T, Guthrie C
  TITLE     The Isy1p component of the NineTeen complex interacts with the 
            ATPase Prp16p to regulate the fidelity of pre-mRNA splicing.
  JOURNAL   Genes Dev. 2005 Aug 15;19(16):1894-904. doi: 10.1101/gad.1336305.
  PUBMED    16103217
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2005-08-15- 15"; volume: "19"; 
            issue: "16"; pages: "1894-904"
COMMENT     Sequence Label: puC19 preACT1(del6) AcAc
FEATURES             Location/Qualifiers
     source          1..3318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     41..60
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        41..59
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(692..709)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(692..708)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(701..723)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1096..1114)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1182..1286
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1287..1355,1356..2147)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1505..1524)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2318..2906
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2807..2826
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3060..3077
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 catcaccgaa acgcgcgagg cccagctggc ttatcgaaat taatacgact cactataggg
       61 agaccggaat tcgagctcgc ccggggatcc ccgggcgagc tcgaattcga gctcgccctt
      121 ttagattttt cacgcttact gcttttttct tcccaagatc gaaaatttac tgaattaaca
      181 atggattctg gtatgttcta gcgcttgcac catcccattt aactgtaaga agaattgcac
      241 ggtcccaatt gctcgagaga tttctctttt accttttttt actatttttc actctcccat
      301 aacctcctat attgactgat ctgtaataac cacgatatta ttggaataaa taggggcttg
      361 aaatttggaa aaaaaaaaaa ctgaaatatt ttcgtgataa gtgatagtga tattcttctt
      421 ttatttgcta ctgtgtctca tgtactaaca tcgattgctt cattcttttt gttgctatat
      481 tatatgttta cacgttgctg ctttggttat tgataacggt tctggtatgt gtaaagccgg
      541 ttttgccggt gacgacgctc ctcgtgctgt cttcccatct atcgtcggta gaccaagaca
      601 ccaaggtatc atggtcggta tgggtcaaaa agactcctac gttggtgatg aagggggatc
      661 ctctagagtc gacctgcagc ccaagcttgg cactggccgt cgttttacaa cgtcgtgact
      721 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      781 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      841 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
      901 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
      961 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     1021 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     1081 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     1141 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     1201 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     1261 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     1321 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     1381 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     1441 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     1501 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     1561 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1621 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1681 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1741 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1801 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1861 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1921 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1981 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     2041 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     2101 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     2161 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     2221 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     2281 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2341 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2401 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2461 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2521 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2581 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2641 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2701 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2761 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2821 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2881 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2941 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3001 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3061 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     3121 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgcgctac
     3181 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     3241 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     3301 gtcagaggtt ttcaccgt
//
