LOCUS       sequence-218074-        3436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Villa T, Guthrie C
  TITLE     The Isy1p component of the NineTeen complex interacts with the 
            ATPase Prp16p to regulate the fidelity of pre-mRNA splicing.
  JOURNAL   Genes Dev. 2005 Aug 15;19(16):1894-904. doi: 10.1101/gad.1336305.
  PUBMED    16103217
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1101/gad"; 
            journalName: "Genes Dev"; date: "2005-08-15- 15"; volume: "19"; 
            issue: "16"; pages: "1894-904"
COMMENT     Sequence Label: puC19 preACT1(del6) short BP-3'SS
FEATURES             Location/Qualifiers
     source          1..3436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     4..21
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5..21
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(605..621)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(653..671)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(692..708)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(692..708)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(705..727)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    716..732
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(740..746,747..764,765..770))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    785..806
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(923..940)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1094..1682)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1174..1193)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1853..2644,2645..2713))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2476..2495
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2714..2818)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2844..3299)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2981..3002)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3193..3212
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(3426..3436,1..12)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga attggagctc
       61 gcccctttta gatttttcac gcttactgct tttttcttcc caagatcgaa aatttactga
      121 attaacaatg gattctggta tgttctagcg cttgcaccat cccatttaac tgtaagaaga
      181 attgcacggt cccaattgct cgagagattt ctcttttacc tttttttact atttttcact
      241 ctcccataac ctcctatatt gactgatctg taataaccac gatattattg gaataaatag
      301 gggcttgaaa tttggaaaaa aaaaaaaact gaaatatttt cgtgataagt gatagtgata
      361 ttcttctttt atttgctact gtgtctcatg tactaacatc gattataggt tgctgctttg
      421 gttattgata acggttctgg tatgtgtaaa gccggttttg ccggtgacga cgctcctcgt
      481 gctgtcttcc catctatcgt cggtagacca agacaccaag gtatcatggt cggtatgggt
      541 caaaaagact cctacgttgg tgatgaaggg gatcctctag agtcgacctg cagcccaagc
      601 ttatcgatac cgtcgacctc gtcgaggggg ggcccggtac cagcttttgt tccctttagt
      661 gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
      721 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      781 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
      841 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
      901 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      961 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1021 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1081 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1141 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1201 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1261 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1321 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1381 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1441 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1501 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1561 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1621 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1681 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1741 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1801 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     1861 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     1921 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     1981 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2041 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2101 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2161 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2221 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2281 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2341 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2401 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2461 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2521 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     2581 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     2641 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     2701 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     2761 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     2821 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     2881 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     2941 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     3001 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     3061 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     3121 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     3181 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     3241 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     3301 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     3361 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     3421 gttttcccag tcacga
//
