LOCUS       sequence-218123-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pTDH3 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK009
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        126..792
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     662..685
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     terminator      complement(815..849)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
       61 ctttcctgcg ttatcccctg attctgtgga taaccgtagt cggtctcaaa cgcagttcga
      121 gtttatcatt atcaatactg ccatttcaaa gaatacgtaa ataattaata gtagtgattt
      181 tcctaacttt atttagtcaa aaaattagcc ttttaattct gctgtaaccc gtacatgccc
      241 aaaatagggg gcgggttaca cagaatatat aacatcgtag gtgtctgggt gaacagttta
      301 ttcctggcat ccactaaata taatggagcc cgctttttaa gctggcatcc agaaaaaaaa
      361 agaatcccag caccaaaata ttgttttctt caccaaccat cagttcatag gtccattctc
      421 ttagcgcaac tacagagaac aggggcacaa acaggcaaaa aacgggcaca acctcaatgg
      481 agtgatgcaa cctgcctgga gtaaatgatg acacaaggca attgacccac gcatgtatct
      541 atctcatttt cttacacctt ctattacctt ctgctctctc tgatttggaa aaagctgaaa
      601 aaaaaggttg aaaccagttc cctgaaatta ttcccctact tgactaataa gtatataaag
      661 acggtaggta ttgattgtaa ttctgtaaat ctatttctta aacttcttaa attctacttt
      721 tatagttagt ctttttttta gttttaaaac accaagaact tagtttcgaa taaacacaca
      781 taaacaaaca aaagatctat gtgagaccag accaataaaa aacgcccggc ggcaaccgag
      841 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
      901 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
      961 cattaagcat tctgccgaca tggaaagcca tcacaaacgg catgatgaac ctgaatcgcc
     1021 agcggcatca
//
