LOCUS       sequence-218145-       12266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK assembled broad-host-range plasmid encoding inducible GFP driven
            by T7 polymerase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12266)
  AUTHORS   Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar 
            S, Davies BW, Ellington AD, Moran NA, Barrick JE
  TITLE     Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit 
            for Modular Assembly of Broad-Host-Range Plasmids.
  JOURNAL   ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 
            10.1021/acssynbio.7b00399. Epub 2018 Apr 13.
  PUBMED    29608282
REFERENCE   2  (bases 1 to 12266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00399"; journalName: "ACS Synth Biol"; date: 
            "2018-05-18- 18"; volume: "7"; issue: "5"; pages: "1279-1290"
COMMENT     Sequence Label: pBTK550d
FEATURES             Location/Qualifiers
     source          1..12266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      9..57
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     terminator      complement(320..367)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     363..381
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     protein_bind    1166..1190
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(1194..1213)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1195..1213)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4078..4089
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    4094..4110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4118..4124,4125..4142,4143..4148))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      4650..5044
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            5386..5473
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             6712..7683
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             8194..9033
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             9020..9871
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             10251..11042
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             11173..12255
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(11192..11211)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gctgataaac cgatacaatt aaaggctcct tttggagcct ttttttttgg agattttcaa
       61 catgaagaaa ttattatttg atgatcagat agcggcgggg aactgccaga catcaaataa
      121 aacaaaaggc tcagtcggaa gactgggcct tttgttttat ctgttgtttg tcggtgaaca
      181 ctctcccggt tgtaccctcc atcgcctcgc attctcggat gtcctggggg ttcgtaacat
      241 ctctgtaact gctggggtcc ttgtgatcct gactatgggg tggttttcag ggatggcagc
      301 atatagttcc tcctttcagc aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt
      361 atgctagtta ttgctcagcg gtggcagcag ccaactcagc ttcctttcgg gctttgttag
      421 cagggattta tttgtatagt tcatccatgc cgtgtgtaat cccagcagcc gtgacaaact
      481 caagaaggac catgtggtcg cgcttttcgt tgggatcttt cgaaagggca gattgagtgg
      541 acaggtaatg gttgtctggt aacagcacag gcccatcgcc aattggggtg ttttgttggt
      601 aatggtcggc tagttgaacg cttccatcct caatgttgtg tctgatcttg aagttaacct
      661 tgataccgtt cttttgcttg tcggccataa tgtacacatt atgggagtta tagttatatt
      721 ccagtttgtg gcctaaaata tttccatctt ccttaaagtc aatacccttt aactcgattc
      781 tattaacaag ggtatcaccc tcgaacttca cttcggcgcg tgtcttatag ttgccgtcat
      841 ccttaaaaaa gatggttctc tcctgtacat agccttcggg cattgcactc ttgaaaaagt
      901 cgtgctgttt catgtgatct gggtagcgcg caaagcactg aaccccatac ccgaacgtag
      961 tgaccagtgt tggccatgga acaggtagtt tacccgtggt gcaaataaat ttaagggtca
     1021 gcttgccata tgttgcgtcg ccttcaccct ctccactgac agagaacttg tgcccattaa
     1081 catcaccgtc taattccacg aggattggga caactcctgt gaaaagctct tctcctttac
     1141 tcatatatct cctctgtttc tgtatggaat tgttatccgc tcacaattcc tctccctata
     1201 gtgagtcgta ttaatttgac agtcattcat ctttctgccc ctccaaaagc aaaaacccgc
     1261 cgaagcgggt ttttacgtaa atcaggtgaa actgaccgac gttcgtctgc aattatcggc
     1321 cagttcagca gctgacaaat gctctttccc taaactcccc ccataaaaaa acccgccgaa
     1381 gcgggttttt acgttatttg cggattaacg attacggatt tacgcgaacg cgaagtccga
     1441 ctctaagatg tcacggaggt tcaagttacc tttagccgga agtgctggca ttttgtccaa
     1501 ttgagactcg tgcaactggt cagcgaactg gtcgtagaaa tcagccagta catcacaaga
     1561 ctcatatgtg tcaaccatag tttcgcgcac tgctttgaac aggttcgcag cgtcagccgg
     1621 aatggtaccg aaggagtcgt gaatcagtgc aaaagattcg attccgtact tctcgtgtgc
     1681 ccacactaca gtcttacgaa ggtggctacc gtcttggctg tgtacaaagt taggagcgat
     1741 accagactcc tgtttgtgtg catcaatctc gctatctttg ttggtgttaa tggtaggctg
     1801 taagcggaac tgaccgagga acatcaggtt caagcgcgtc tgaataggct tcttgtattc
     1861 ctgccacaca gggaaaccat caggagttac ccaatgcaca gcgcaacgct tgcgaagaat
     1921 ctctccagtc ttcttatctt tgacctcagc agccagcagc ttagcagcag acttaagcca
     1981 gttcattgct tcaaccgcag ctaccaccgt cacgctcaca gattcccaaa tcagcttagc
     2041 catgtatcca gcagcctgat tcggctgagt gaacatcaga cccttgccgg aatcaatagc
     2101 tggctgaatg gtatcttcca gcacttgttg acggaagccg aactctttgg acccgtaagc
     2161 cagcgtcatg actgaacgct tagtcacact gcgagtaaca ccgtaagcca gccattgacc
     2221 agccagtgcc ttagtgccca gcttgacttt ctcagagatt tcaccagtgt tctcatcggt
     2281 cacggtaact acttcgttat cggtcccatt gattgcgtct gcttgtagaa tctcgttgac
     2341 tttcttagca acaatcccgt agatgtcctg aacggtttca ctaggaagca agttaaccgc
     2401 gcgaccacct acctcatctc ggagcatcgc ggagaagtgc tggatgccag agcaagaccc
     2461 gtcaaacgcc agcggaaggg agcagttata gctcaggccg tggtgctgta ccccagcgta
     2521 ctcaaagcag aacgcaagga agcagaacgg agaatcttgc tcagcccacc aagtgttctc
     2581 cagtggagac ttagcgcaag ccatgatgtt ctcgtggttt tcctcaatga acttgatgcg
     2641 ctcagggaac ggaaccttat cgacacccgc acagtttgca ccgtggattt tcagccagta
     2701 gtaaccttcc ttaccgattg gtttaccttt cgccagcgta agcagtcctt tggtcatatc
     2761 gttaccttgc gggttgaaca ttgacacagc gtaaacacga ccgcgccagt ccatgttgta
     2821 agggaaccag atggccttat ggttagcaaa cttattggct tgctcaagca tgaactcaag
     2881 gctgatacgg cgagacttgc gagccttgtc cttgcggtac acagcagcgg cagcacgttt
     2941 ccacgcggtg agagcctcag gattcatgtc gatgtcttcc ggtttcatcg ggagttcttc
     3001 acgctcaatc gcagggatgt cctcgaccgg acaatgcttc cacttggtga ttacgttggc
     3061 gaccgctagg actttcttgt tgattttcca tgcggtgttt tgcgcaatgt taatcgcttt
     3121 gtacacctca ggcatgtaaa cgtcttcgta gcgcatcagt gctttcttac tgtgagtacg
     3181 caccagcgcc agaggacgac gaccgttagc ccaatagcca ccaccagtaa tgccagtcca
     3241 cggcttagga ggaactacgc aaggttggaa catcggagag atgccagcca gcgcacctgc
     3301 acgggttgcg atagcctcag cgtattcagg tgcgagttcg atagtctcag agtcttgacc
     3361 tactacgcca gcattttggc ggtgtaagct aaccattccg gttgactcaa tgagcatctc
     3421 gatgcagcgt actcctacat gaatagagtc ttccttatgc cacgaagacc acgcctcgcc
     3481 accgagtaga cccttagaga gcatgtcagc ctcgacaact tgcataaatg ctttcttgta
     3541 gacgtgccct acgcgcttgt tgagttgttc ctcaacgttt ttcttgaagt gcttagcttc
     3601 aaggtcacgg atacgaccga agcgagcctc gtcctcaatg gcccgaccga ttgcgcttgc
     3661 tacagcctga acggttgtat tgtcagcact ggttaggcaa gccagagtgg tcttaatggt
     3721 gatgtacgct acggcttccg gcttgatttc ttgcaggaac tggaaggctg tcgggcgctt
     3781 gccgcgctta gctttcactt cctcaaacca gtcgttgatg cgtgcaatca tcttagggag
     3841 tagggtagtg atgagaggct tggcggcagc gttatccgca acctcaccag ctttaagttg
     3901 acgctcaaac atcttgcgga agcgtgcttc acccatctcg taagactcat gctcaagggc
     3961 caactgttcg cgagctaaac gctcaccgta atggtcagcc agagtgttga acgggatagc
     4021 agccagttcg atgtcagaga agtcgttctt agcgatgtta atcgtgttca tagttaattt
     4081 ctcctcttta aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4141 agtgtaaacg ttgaggggca ggttgtcttg agggtgacag agcaagaccc tgtctagatg
     4201 cgaattcagg ggcctgtcag accaagttta ctcatatata ctttagattg atttctgaaa
     4261 gcgaccaggt gctcggcgtg gcaagactcg cagcgaaccc gtagaaagcc atgctccagc
     4321 cgcccgcatt ggagaaattc ttcaaattcc cgttgcacat agcccggcaa ttcctttccc
     4381 tgctctgcca taagcgcagc gaatgccggg taatactcgt caacgatctg atagagaagg
     4441 gtttgctcgg gtcggtggct ctggtaacga ccagtatccc gatcccggct ggccgtcctg
     4501 gccgccacat gaggcatgtt ccgcgtcctt gcaatactgt gtttacatac agtctatcgc
     4561 ttagcggaaa gttcttttac cctcagccga aatgcctgcc gttgctagac attgccagcc
     4621 agtgcccgtc actcccgtac taactgtcac gaacccctgc aataactgtc acgcccccct
     4681 gcaataactg tcacgaaccc ctgcaataac tgtcacgccc ccaaacctgc aaacccagca
     4741 ggggcggggg ctggcggggt gttggaaaaa tccatccatg attatctaag aataatccac
     4801 taggcgcggt tatcagcgcc cttgtggggc gctgctgccc ttgcccaata tgcccggcca
     4861 gaggccggat agctggtcta ttcgctgcgc taggctacac accgccccac cgctgcgcgg
     4921 cagggggaaa ggcgggcaaa gcccgctaaa ccccacacca aaccccgcag aaatacgctg
     4981 gagcgctttt agccgcttta gcggcctttc cccctacccg aagggtgggg gcgcgtgtgc
     5041 agccccgcag ggcctgtctc ggtcgatcat tcagcccggc tcatccttct ggcgtggcgg
     5101 cagaccgaac aaggcgcggt cgtggtcgcg ttcaaggtac gcatccattg ccgccatgag
     5161 ccgatcctcc ggccactcgc tgctgttcac cttggccaaa atcatggccc ccaccagcac
     5221 cttgcgcctt gtttcgttct tgcgctcttg ctgctgttcc cttgcccgca cccgctgaat
     5281 ttcggcattg attcgcgctc gttgttcttc gagcttggcc agccgatccg ccgccttgtt
     5341 gctcccctta accatcttga caccccattg ttaatgtgct gtctcgtagg ctatcatgga
     5401 ggcacagcgg cggcaatccc gaccctactt tgtaggggag ggcgcactta ccggtttctc
     5461 ttcgagaaac tggcctaacg gccacccttc gggcggtgcg ctctccgagg gccattgcat
     5521 ggagccgaaa agcaaaagca acagcgaggc agcatggcga tttatcacct tacggcgaaa
     5581 accggcagca ggtcgggcgg ccaatcggcc agggccaagg ccgactacat ccagcgcgaa
     5641 ggcaagtatg cccgcgacat ggatgaagtc ttgcacgccg aatccgggca catgccggag
     5701 ttcgtcgagc ggcccgccga ctactgggat gctgccgacc tgtatgaacg cgccaatggg
     5761 cggctgttca aggaggtcga atttgccctg ccggtcgagc tgaccctcga ccagcagaag
     5821 gcgctggcgt ccgagttcgc ccagcacctg accggtgccg agcgcctgcc gtatacgctg
     5881 gccatccatg ccggtggcgg cgagaacccg cactgccacc tgatgatctc cgagcggatc
     5941 aatgacggca tcgagcggcc cgccgctcag tggttcaagc ggtacaacgg caagaccccg
     6001 gagaagggcg gggcacagaa gaccgaagcg ctcaagccca aggcatggct tgagcagacc
     6061 cgcgaggcat gggccgacca tgccaaccgg gcattagagc gggctggcca cgacgcccgc
     6121 attgaccaca gaacacttga ggcgcagggc atcgagcgcc tgcccggtgt tcacctgggg
     6181 ccgaacgtgg tggagatgga aggccggggc atccgcaccg accgggcaga cgtggccctg
     6241 aacatcgaca ccgccaacgc ccagatcatc gacttacagg aataccggga ggcaatagac
     6301 catgaacgca atcgacagag tgaagaaatc cagaggcatc aacgagttag cggagcagat
     6361 cgaaccgctg gcccagagca tggcgacact ggccgacgaa gcccggcagg tcatgagcca
     6421 gacccagcag gccagcgagg cgcaggcggc ggagtggctg aaagcccagc gccagacagg
     6481 ggcggcatgg gtggagctgg ccaaagagtt gcgggaggta gccgccgagg tgagcagcgc
     6541 cgcgcagagc gcccggagcg cgtcgcgggg gtggcactgg aagctatggc taaccgtgat
     6601 gctggcttcc atgatgccta cggtggtgct gctgatcgca tcgttgctct tgctcgacct
     6661 gacgccactg acaaccgagg acggctcgat ctggctgcgc ttggtggccc gatgaagaac
     6721 gacaggactt tgcaggccat aggccgacag ctcaaggcca tgggctgtga gcgcttcgat
     6781 atcggcgtca gggacgccac caccggccag atgatgaacc gggaatggtc agccgccgaa
     6841 gtgctccaga acacgccatg gctcaagcgg atgaatgccc agggcaatga cgtgtatatc
     6901 aggcccgccg agcaggagcg gcatggtctg gtgctggtgg acgacctcag cgagtttgac
     6961 ctggatgaca tgaaagccga gggccgggag cctgccctgg tagtggaaac cagcccgaag
     7021 aactatcagg catgggtcaa ggtggccgac gccgcaggcg gtgaacttcg ggggcagatt
     7081 gcccggacgc tggccagcga gtacgacgcc gacccggcca gcgccgacag ccgccactat
     7141 ggccgcttgg cgggcttcac caaccgcaag gacaagcaca ccacccgcgc cggttatcag
     7201 ccgtgggtgc tgctgcgtga atccaagggc aagaccgcca ccgctggccc ggcgctggtg
     7261 cagcaggctg gccagcagat cgagcaggcc cagcggcagc aggagaaggc ccgcaggctg
     7321 gccagcctcg aactgcccga gcggcagctt agccgccacc ggcgcacggc gctggacgag
     7381 taccgcagcg agatggccgg gctggtcaag cgcttcggtg atgacctcag caagtgcgac
     7441 tttatcgccg cgcagaagct ggccagccgg ggccgcagtg ccgaggaaat cggcaaggcc
     7501 atggccgagg ccagcccagc gctggcagag cgcaagcccg gccacgaagc ggattacatc
     7561 gagcgcaccg tcagcaaggt catgggtctg cccagcgtcc agcttgcgcg ggccgagctg
     7621 gcacgggcac cggcaccccg ccagcgaggc atggacaggg gcgggccaga tttcagcatg
     7681 tagtgcttgc gttggtactc acgcctgtta tactatgagt actcacgcac agaagggggt
     7741 tttatggaat acgaaaaaag cgcttcaggg tcggtctacc tgatcaaaag tgacaagggc
     7801 tattggttgc ccggtggctt tggttatacg tcaaacaagg ccgaggctgg ccgcttttca
     7861 gtcgctgata tggccagcct taaccttgac ggctgcacct tgtccttgtt ccgcgaagac
     7921 aagcctttcg gccccggcaa gtttctcggt gactgatatg aaagaccaaa aggacaagca
     7981 gaccggcgac ctgctggcca gccctgacgc tgtacgccaa gcgcgatatg ccgagcgcat
     8041 gaaggccaaa gggatgcgtc agcgcaagtt ctggctgacc gacgacgaat acgaggcgct
     8101 gcgcgagtgc ctggaagaac tcagagcggc gcagggcggg ggtagtgacc ccgccagcgc
     8161 ctaaccacca actgcctgca aaggaggcaa tcaatggcta cccataagcc tatcaatatt
     8221 ctggaggcgt tcgcagcagc gccgccaccg ctggactacg ttttgcccaa catggtggcc
     8281 ggtacggtcg gggcgctggt gtcgcccggt ggtgccggta aatccatgct ggccctgcaa
     8341 ctggccgcac agattgcagg cgggccggat ctgctggagg tgggcgaact gcccaccggc
     8401 ccggtgatct acctgcccgc cgaagacccg cccaccgcca ttcatcaccg cctgcacgcc
     8461 cttggggcgc acctcagcgc cgaggaacgg caagccgtgg ctgacggcct gctgatccag
     8521 ccgctgatcg gcagcctgcc caacatcatg gccccggagt ggttcgacgg cctcaagcgc
     8581 gccgccgagg gccgccgcct gatggtgctg gacacgctgc gccggttcca catcgaggaa
     8641 gaaaacgcca gcggccccat ggcccaggtc atcggtcgca tggaggccat cgccgccgat
     8701 accgggtgct ctatcgtgtt cctgcaccat gccagcaagg gcgcggccat gatgggcgca
     8761 ggcgaccagc agcaggccag ccggggcagc tcggtactgg tcgataacat ccgctggcag
     8821 tcctacctgt cgagcatgac cagcgccgag gccgaggaat ggggtgtgga cgacgaccag
     8881 cgccggttct tcgtccgctt cggtgtgagc aaggccaact atggcgcacc gttcgctgat
     8941 cggtggttca ggcggcatga cggcggggtg ctcaagcccg ccgtgctgga gaggcagcgc
     9001 aagagcaagg gggtgccccg tggtgaagcc taagaacaag cacagcctca gccacgtccg
     9061 gcacgacccg gcgcactgtc tggcccccgg cctgttccgt gccctcaagc ggggcgagcg
     9121 caagcgcagc aagctggacg tgacgtatga ctacggcgac ggcaagcgga tcgagttcag
     9181 cggcccggag ccgctgggcg ctgatgatct gcgcatcctg caagggctgg tggccatggc
     9241 tgggcctaat ggcctagtgc ttggcccgga acccaagacc gaaggcggac ggcagctccg
     9301 gctgttcctg gaacccaagt gggaggccgt caccgctgat gccatggtgg tcaaaggtag
     9361 ctatcgggcg ctggcaaagg aaatcggggc agaggtcgat agtggtgggg cgctcaagca
     9421 catacaggac tgcatcgagc gcctttggaa ggtatccatc atcgcccaga atggccgcaa
     9481 gcggcagggg tttcggctgc tgtcggagta cgccagcgac gaggcggacg ggcgcctgta
     9541 cgtggccctg aaccccttga tcgcgcaggc cgtcatgggt ggcggccagc atgtgcgcat
     9601 cagcatggac gaggtgcggg cgctggacag cgaaaccgcc cgcctgctgc accagcggct
     9661 gtgtggctgg atcgaccccg gcaaaaccgg caaggcttcc atagatacct tgtgcggcta
     9721 tgtctggccg tcagaggcca gtggttcgac catgcgcaag cgccgccagc gggtgcgcga
     9781 ggcgttgccg gagctggtcg cgctgggctg gacggtaacc gagttcgcgg cgggcaagta
     9841 cgacatcacc cggcccaagg cggcaggctg actcacgtta agggattttg gtcatgacca
     9901 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
     9961 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
    10021 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
    10081 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
    10141 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
    10201 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
    10261 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
    10321 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
    10381 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
    10441 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
    10501 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
    10561 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcgggt atcttcgagc
    10621 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
    10681 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
    10741 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
    10801 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
    10861 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
    10921 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
    10981 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
    11041 aagcggccgc tcggctttgg cagtttattc ttgacatgta gtgagggggc tggtataatc
    11101 acatagtact gttatacaga aacagaggag atattacggt acgcaagtac acaagataca
    11161 ggagaggtac tcatgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct
    11221 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa
    11281 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg
    11341 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg
    11401 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg
    11461 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg
    11521 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg
    11581 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc
    11641 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca
    11701 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg
    11761 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg
    11821 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc
    11881 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc
    11941 attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc
    12001 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg
    12061 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat
    12121 cagctgttgc cggtgtcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc
    12181 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
    12241 gaaagcgggc agtgatgtaa aagcaa
//
