LOCUS       sequence-218147-        9491 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK assembled broad-host-range plasmid encoding inducible GFP driven
            CP25 promoter with lac operator. Also encodes LacI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9491)
  AUTHORS   Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar 
            S, Davies BW, Ellington AD, Moran NA, Barrick JE
  TITLE     Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit 
            for Modular Assembly of Broad-Host-Range Plasmids.
  JOURNAL   ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 
            10.1021/acssynbio.7b00399. Epub 2018 Apr 13.
  PUBMED    29608282
REFERENCE   2  (bases 1 to 9491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00399"; journalName: "ACS Synth Biol"; date: 
            "2018-05-18- 18"; volume: "7"; issue: "5"; pages: "1279-1290"
COMMENT     Sequence Label: pBTK552
FEATURES             Location/Qualifiers
     source          1..9491
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(451..1302)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(1289..2128)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(2639..3610)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            4849..4936
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      5278..5672
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     protein_bind    6429..6453
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(7491..7539)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(7559..8641)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8603..8622
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             complement(join(8771..9491,1..71))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc
       61 gcttccctca taatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
      121 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga
      181 ggcatagact gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg
      241 ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg
      301 cattacagct tacgaaccga acaggcttat gtccactggg ttcgtgcctt catccgtttc
      361 cacggtgtgc gtcacccggc aaccttgggt agcagcgaag tcgaggcatt tctgtcctgg
      421 ctggtcatga ccaaaatccc ttaacgtgag tcagcctgcc gccttgggcc gggtgatgtc
      481 gtacttgccc gccgcgaact cggttaccgt ccagcccagc gcgaccagct ccggcaacgc
      541 ctcgcgcacc cgctggcggc gcttgcgcat ggtcgaacca ctggcctctg acggccagac
      601 atagccgcac aaggtatcta tggaagcctt gccggttttg ccggggtcga tccagccaca
      661 cagccgctgg tgcagcaggc gggcggtttc gctgtccagc gcccgcacct cgtccatgct
      721 gatgcgcaca tgctggccgc cacccatgac ggcctgcgcg atcaaggggt tcagggccac
      781 gtacaggcgc ccgtccgcct cgtcgctggc gtactccgac agcagccgaa acccctgccg
      841 cttgcggcca ttctgggcga tgatggatac cttccaaagg cgctcgatgc agtcctgtat
      901 gtgcttgagc gccccaccac tatcgacctc tgccccgatt tcctttgcca gcgcccgata
      961 gctacctttg accaccatgg catcagcggt gacggcctcc cacttgggtt ccaggaacag
     1021 ccggagctgc cgtccgcctt cggtcttggg ttccgggcca agcactaggc cattaggccc
     1081 agccatggcc accagccctt gcaggatgcg cagatcatca gcgcccagcg gctccgggcc
     1141 gctgaactcg atccgcttgc cgtcgccgta gtcatacgtc acgtccagct tgctgcgctt
     1201 gcgctcgccc cgcttgaggg cacggaacag gccgggggcc agacagtgcg ccgggtcgtg
     1261 ccggacgtgg ctgaggctgt gcttgttctt aggcttcacc acggggcacc cccttgctct
     1321 tgcgctgcct ctccagcacg gcgggcttga gcaccccgcc gtcatgccgc ctgaaccacc
     1381 gatcagcgaa cggtgcgcca tagttggcct tgctcacacc gaagcggacg aagaaccggc
     1441 gctggtcgtc gtccacaccc cattcctcgg cctcggcgct ggtcatgctc gacaggtagg
     1501 actgccagcg gatgttatcg accagtaccg agctgccccg gctggcctgc tgctggtcgc
     1561 ctgcgcccat catggccgcg cccttgctgg catggtgcag gaacacgata gagcacccgg
     1621 tatcggcggc gatggcctcc atgcgaccga tgacctgggc catggggccg ctggcgtttt
     1681 cttcctcgat gtggaaccgg cgcagcgtgt ccagcaccat caggcggcgg ccctcggcgg
     1741 cgcgcttgag gccgtcgaac cactccgggg ccatgatgtt gggcaggctg ccgatcagcg
     1801 gctggatcag caggccgtca gccacggctt gccgttcctc ggcgctgagg tgcgccccaa
     1861 gggcgtgcag gcggtgatga atggcggtgg gcgggtcttc ggcgggcagg tagatcaccg
     1921 ggccggtggg cagttcgccc acctccagca gatccggccc gcctgcaatc tgtgcggcca
     1981 gttgcagggc cagcatggat ttaccggcac caccgggcga caccagcgcc ccgaccgtac
     2041 cggccaccat gttgggcaaa acgtagtcca gcggtggcgg cgctgctgcg aacgcctcca
     2101 gaatattgat aggcttatgg gtagccattg attgcctcct ttgcaggcag ttggtggtta
     2161 ggcgctggcg gggtcactac ccccgccctg cgccgctctg agttcttcca ggcactcgcg
     2221 cagcgcctcg tattcgtcgt cggtcagcca gaacttgcgc tgacgcatcc ctttggcctt
     2281 catgcgctcg gcatatcgcg cttggcgtac agcgtcaggg ctggccagca ggtcgccggt
     2341 ctgcttgtcc ttttggtctt tcatatcagt caccgagaaa cttgccgggg ccgaaaggct
     2401 tgtcttcgcg gaacaaggac aaggtgcagc cgtcaaggtt aaggctggcc atatcagcga
     2461 ctgaaaagcg gccagcctcg gccttgtttg acgtataacc aaagccaccg ggcaaccaat
     2521 agcccttgtc acttttgatc aggtagaccg accctgaagc gcttttttcg tattccataa
     2581 aacccccttc tgtgcgtgag tactcatagt ataacaggcg tgagtaccaa cgcaagcact
     2641 acatgctgaa atctggcccg cccctgtcca tgcctcgctg gcggggtgcc ggtgcccgtg
     2701 ccagctcggc ccgcgcaagc tggacgctgg gcagacccat gaccttgctg acggtgcgct
     2761 cgatgtaatc cgcttcgtgg ccgggcttgc gctctgccag cgctgggctg gcctcggcca
     2821 tggccttgcc gatttcctcg gcactgcggc cccggctggc cagcttctgc gcggcgataa
     2881 agtcgcactt gctgaggtca tcaccgaagc gcttgaccag cccggccatc tcgctgcggt
     2941 actcgtccag cgccgtgcgc cggtggcggc taagctgccg ctcgggcagt tcgaggctgg
     3001 ccagcctgcg ggccttctcc tgctgccgct gggcctgctc gatctgctgg ccagcctgct
     3061 gcaccagcgc cgggccagcg gtggcggtct tgcccttgga ttcacgcagc agcacccacg
     3121 gctgataacc ggcgcgggtg gtgtgcttgt ccttgcggtt ggtgaagccc gccaagcggc
     3181 catagtggcg gctgtcggcg ctggccgggt cggcgtcgta ctcgctggcc agcgtccggg
     3241 caatctgccc ccgaagttca ccgcctgcgg cgtcggccac cttgacccat gcctgatagt
     3301 tcttcgggct ggtttccact accagggcag gctcccggcc ctcggctttc atgtcatcca
     3361 ggtcaaactc gctgaggtcg tccaccagca ccagaccatg ccgctcctgc tcggcgggcc
     3421 tgatatacac gtcattgccc tgggcattca tccgcttgag ccatggcgtg ttctggagca
     3481 cttcggcggc tgaccattcc cggttcatca tctggccggt ggtggcgtcc ctgacgccga
     3541 tatcgaagcg ctcacagccc atggccttga gctgtcggcc tatggcctgc aaagtcctgt
     3601 cgttcttcat cgggccacca agcgcagcca gatcgagccg tcctcggttg tcagtggcgt
     3661 caggtcgagc aagagcaacg atgcgatcag cagcaccacc gtaggcatca tggaagccag
     3721 catcacggtt agccatagct tccagtgcca cccccgcgac gcgctccggg cgctctgcgc
     3781 ggcgctgctc acctcggcgg ctacctcccg caactctttg gccagctcca cccatgccgc
     3841 ccctgtctgg cgctgggctt tcagccactc cgccgcctgc gcctcgctgg cctgctgggt
     3901 ctggctcatg acctgccggg cttcgtcggc cagtgtcgcc atgctctggg ccagcggttc
     3961 gatctgctcc gctaactcgt tgatgcctct ggatttcttc actctgtcga ttgcgttcat
     4021 ggtctattgc ctcccggtat tcctgtaagt cgatgatctg ggcgttggcg gtgtcgatgt
     4081 tcagggccac gtctgcccgg tcggtgcgga tgccccggcc ttccatctcc accacgttcg
     4141 gccccaggtg aacaccgggc aggcgctcga tgccctgcgc ctcaagtgtt ctgtggtcaa
     4201 tgcgggcgtc gtggccagcc cgctctaatg cccggttggc atggtcggcc catgcctcgc
     4261 gggtctgctc aagccatgcc ttgggcttga gcgcttcggt cttctgtgcc ccgcccttct
     4321 ccggggtctt gccgttgtac cgcttgaacc actgagcggc gggccgctcg atgccgtcat
     4381 tgatccgctc ggagatcatc aggtggcagt gcgggttctc gccgccaccg gcatggatgg
     4441 ccagcgtata cggcaggcgc tcggcaccgg tcaggtgctg ggcgaactcg gacgccagcg
     4501 ccttctgctg gtcgagggtc agctcgaccg gcagggcaaa ttcgacctcc ttgaacagcc
     4561 gcccattggc gcgttcatac aggtcggcag catcccagta gtcggcgggc cgctcgacga
     4621 actccggcat gtgcccggat tcggcgtgca agacttcatc catgtcgcgg gcatacttgc
     4681 cttcgcgctg gatgtagtcg gccttggccc tggccgattg gccgcccgac ctgctgccgg
     4741 ttttcgccgt aaggtgataa atcgccatgc tgcctcgctg ttgcttttgc ttttcggctc
     4801 catgcaatgg ccctcggaga gcgcaccgcc cgaagggtgg ccgttaggcc agtttctcga
     4861 agagaaaccg gtaagtgcgc cctcccctac aaagtagggt cgggattgcc gccgctgtgc
     4921 ctccatgata gcctacgaga cagcacatta acaatggggt gtcaagatgg ttaaggggag
     4981 caacaaggcg gcggatcggc tggccaagct cgaagaacaa cgagcgcgaa tcaatgccga
     5041 aattcagcgg gtgcgggcaa gggaacagca gcaagagcgc aagaacgaaa caaggcgcaa
     5101 ggtgctggtg ggggccatga ttttggccaa ggtgaacagc agcgagtggc cggaggatcg
     5161 gctcatggcg gcaatggatg cgtaccttga acgcgaccac gaccgcgcct tgttcggtct
     5221 gccgccacgc cagaaggatg agccgggctg aatgatcgac cgagacaggc cctgcggggc
     5281 tgcacacgcg cccccaccct tcgggtaggg ggaaaggccg ctaaagcggc taaaagcgct
     5341 ccagcgtatt tctgcggggt ttggtgtggg gtttagcggg ctttgcccgc ctttccccct
     5401 gccgcgcagc ggtggggcgg tgtgtagcct agcgcagcga atagaccagc tatccggcct
     5461 ctggccgggc atattgggca agggcagcag cgccccacaa gggcgctgat aaccgcgcct
     5521 agtggattat tcttagataa tcatggatgg atttttccaa caccccgcca gcccccgccc
     5581 ctgctgggtt tgcaggtttg ggggcgtgac agttattgca ggggttcgtg acagttattg
     5641 caggggggcg tgacagttat tgcaggggtt cgtgacagtt agtacgggag tgacgggcac
     5701 tggctggcaa tgtctagcaa cggcaggcat ttcggctgag ggtaaaagaa ctttccgcta
     5761 agcgatagac tgtatgtaaa cacagtattg caaggacgcg gaacatgcct catgtggcgg
     5821 ccaggacggc cagccgggat cgggatactg gtcgttacca gagccaccga cccgagcaaa
     5881 cccttctcta tcagatcgtt gacgagtatt acccggcatt cgctgcgctt atggcagagc
     5941 agggaaagga attgccgggc tatgtgcaac gggaatttga agaatttctc caatgcgggc
     6001 ggctggagca tggctttcta cgggttcgct gcgagtcttg ccacgccgag cacctggtcg
     6061 ctttcagaaa tcaatctaaa gtatatatga gtaaacttgg tctgacaggc ccctgaattc
     6121 gcatctagac tgatgagacg tggtagagcc acaaacagcc ggtacaagca acgatctcca
     6181 ggaccatctg aatcatgcgc ggatgacacg aactcacgac ggcgatcaca gacattaacc
     6241 cacagtacag acactgcgac aacgtggcaa ttcgtcgcaa tacaacgtcg gtcagtttca
     6301 cctgatttac gtaaaaaccc gcttcggcgg gtttttgctt ttggaggggc agaaagatga
     6361 atgactgtcc tttggcagtt tattcttgac atgtagtgag ggggctggta taatcacata
     6421 gtactgttgg aattgtgagc ggataacaat tccatacaga aacagaggag atattacata
     6481 tgagtaaagg agaagagctt ttcacaggag ttgtcccaat cctcgtggaa ttagacggtg
     6541 atgttaatgg gcacaagttc tctgtcagtg gagagggtga aggcgacgca acatatggca
     6601 agctgaccct taaatttatt tgcaccacgg gtaaactacc tgttccatgg ccaacactgg
     6661 tcactacgtt cgggtatggg gttcagtgct ttgcgcgcta cccagatcac atgaaacagc
     6721 acgacttttt caagagtgca atgcccgaag gctatgtaca ggagagaacc atctttttta
     6781 aggatgacgg caactataag acacgcgccg aagtgaagtt cgagggtgat acccttgtta
     6841 atagaatcga gttaaagggt attgacttta aggaagatgg aaatatttta ggccacaaac
     6901 tggaatataa ctataactcc cataatgtgt acattatggc cgacaagcaa aagaacggta
     6961 tcaaggttaa cttcaagatc agacacaaca ttgaggatgg aagcgttcaa ctagccgacc
     7021 attaccaaca aaacacccca attggcgatg ggcctgtgct gttaccagac aaccattacc
     7081 tgtccactca atctgccctt tcgaaagatc ccaacgaaaa gcgcgaccac atggtccttc
     7141 ttgagtttgt cacggctgct gggattacac acggcatgga tgaactatac aaataaatcc
     7201 gtaatcgtta atccgcaaat aacgtaaaaa cccgcttcgg cgggtttttt tatgggggga
     7261 gtttagggaa agagcatttg tcagctgcgt ctcaagcagt tacagagatg ttacgaaccc
     7321 ccaggacatc cgagaatgcg aggcgatgga gggtacaacc gggagagtgt tcaccgacaa
     7381 acaacagata aaacaaaagg cccagtcttc cgactgagcc ttttgtttta tttgatgtct
     7441 ggcagttccc cgccgctatc tgatcatcaa ataataattt cttcatgttg aaaatctcca
     7501 aaaaaaaagg ctccaaaagg agcctttaat tgtatcggtt tatcagcttg cttttacatc
     7561 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7621 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaca
     7681 ccggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
     7741 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac
     7801 atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc
     7861 cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg
     7921 cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac
     7981 tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc
     8041 agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
     8101 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga
     8161 aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
     8221 tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
     8281 cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
     8341 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
     8401 cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
     8461 actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg
     8521 ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg
     8581 aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca
     8641 tgagtacctc tcctgtatct tgtgtacttg cgtaccgaat atctcctctg tttctgtata
     8701 acagtactat gtgattatac cagccccctc actacatgtc aagaataaac tgccaaagcc
     8761 gagcggccgc ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt
     8821 cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc
     8881 tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag
     8941 cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa
     9001 gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt
     9061 catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg
     9121 gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt
     9181 cgatcgtggc tggctcgaag atacccgcaa gaatgtcatt gcgctgccat tctccaaatt
     9241 gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga
     9301 cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt
     9361 tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa
     9421 tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca
     9481 acgtcggttc g
//
