LOCUS       sequence-218150-        1154 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control vector expressing GFP alone without any consensus repeats.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Rossl A, Denoncourt A, Lin MS, Downey M
  TITLE     A synthetic non-histone substrate to study substrate targeting by 
            the Gcn5 HAT and sirtuin HDACs.
  JOURNAL   J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 
            10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
  PUBMED    30804216
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.006051"; journalName: "J Biol Chem"; date: 
            "2019-04-19- 19"; volume: "294"; issue: "16"; pages: "6227-6239"
COMMENT     Sequence Label: GFP-X0
FEATURES             Location/Qualifiers
     source          1..1154
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     71..91
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(143..159)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(144..160)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      162..409
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(178..196)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(431..449)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(470..486)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(470..486)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(483..505)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    494..510
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(518..524,525..542,543..548))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    563..584
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(701..718)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(952..971)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cccggttccg aacattacct gtcacacaat ctgccctttc gaaagatccc aacgaaaaga
       61 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
      121 aactatacaa ataaaagctt atcgataccg tcgacctcga gtcatgtaat tagttatgtc
      181 acgcttacat tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca
      241 acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt
      301 tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact
      361 gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct ttaatttgcg gccggtacca
      421 gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt
      481 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
      541 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
      601 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
      661 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
      721 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
      781 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      841 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc ccctgacgag
      901 catcacaaaa atcgacgctc aagtcagagt ggcgaaaccc gacaggacta taaagatacc
      961 aggcgtttcc ccctggaagc tccctcgtgc gctctctgtt ccgaccctgc cgcttaccga
     1021 tactgtccgg ccttttctcc cttcggagcg tgcgcttttc tcatagctca cgctgtagta
     1081 tctcagttcg tgtaagtcgt cgcttcaagc tgactgatgc acgagcccct cggatcaagc
     1141 tcggaacccg tcgt
//
