LOCUS       sequence-218151-        1143 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Control vector expressing GFP alone without any consensus repeats.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Rossl A, Denoncourt A, Lin MS, Downey M
  TITLE     A synthetic non-histone substrate to study substrate targeting by 
            the Gcn5 HAT and sirtuin HDACs.
  JOURNAL   J Biol Chem. 2019 Apr 19;294(16):6227-6239. doi: 
            10.1074/jbc.RA118.006051. Epub 2019 Feb 25.
  PUBMED    30804216
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.006051"; journalName: "J Biol Chem"; date: 
            "2019-04-19- 19"; volume: "294"; issue: "16"; pages: "6227-6239"
COMMENT     Sequence Label: GFP-X0
FEATURES             Location/Qualifiers
     source          1..1143
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     127..145
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     163..179
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     164..180
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(232..252)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(927..943)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(927..943)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 aaggttaacg aacttgaata taaataacgt tcttaatact aacataacta taaaaaaata
       61 aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag cggatgtggg
      121 gggagggcgt gaatgtaagc gtgacataac taattacatg actcgaggtc gacggtatcg
      181 ataagctttt atttgtatag ttcatccatg ccatgtgtaa tcccagcagc tgttacaaac
      241 tcaagaagga ccatgtggtc tctcttttcg ttgggatctt tcgaaagggc agattgtgtg
      301 gacaggtaat ggttgtctgg taaaaggaca gggccatcgc caattggagt attttgttga
      361 taatggtctg ctagttgaac gcttccatct tcaatgttgt gtctaatttt gaagttaact
      421 ttgattccat tcttttgttt gtctgccatg atgtatacat tgtgtgagtt atagttgtat
      481 tccaatttgt gtccaagaat gtttccatct tctttaaaat caataccttt taactcgatt
      541 ctattaacaa gggtatcacc ttcaaacttg acttcagcac gtgtcttgta gttcccgtca
      601 tctttgaaaa atatagttct ttcctgtaca taaccttcgg gcatggcact cttgaaaaag
      661 tcatgccgtt tcatatgatc tgggtatctt gaaaagcatt gaacaccata agtgaaagta
      721 gtgacaagtg ttggccatgg aacaggtagt tttccagtag tgcaaataaa tttaagggta
      781 agttttccgt atgttgcatc accttcaccc tctccactga cagaaaattt gtgcccatta
      841 acatcaccat ctaattcaac aagaattggg gacaactcca gtgaaaagtt cttctccttt
      901 actcatgaat tcctgcagcc cggggggatc cactagttct agagcggcca gcttgggagt
      961 tgatttgtat gcttggtata gctttgaaat atttgtgcag aaaaagaaac agggaaggaa
     1021 agggaacgag actaattgac gaaggaacaa aagatcatta tttgcaggtc taatttatac
     1081 ctggatagca gacagctacc tttttttatt cgatcagtac tgatgaaagc tgtattatac
     1141 cgt
//
