LOCUS       sequence-21816-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Tournier C, Whitmarsh AJ, Cavanagh J, Barrett T, Davis RJ
  TITLE     The MKK7 gene encodes a group of c-Jun NH2-terminal kinase kinases.
  JOURNAL   Mol Cell Biol. 1999 Feb . 19(2):1569-81.
  PUBMED    9891090
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1999 Feb . 19(2):1569-81."
COMMENT     Sequence Label: pCDNA3 Flag MKK7g1
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(293..304)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt cggcagccaa catggactat aaggacgatg atgacaaaat
      121 ggcggcgtcc tccctggagc agaagctgtc ccgcctggaa gccaagctga agcaggagaa
      181 ccgtgaggcc cgcaggagga tcgacctcaa cttggatatc agcccacagc ggcccaggcc
      241 cattattgtg atcactctaa gccctgctcc tgccccgtcc cagcgagcag ccctgcaact
      301 cccactggcc aacgatgggg gcagccgctc accatcctca gagagctccc cacagcaccc
      361 tacacccccc acccggcccc gccacatgct ggggctccca tcaaccttgt tcacaccgcg
      421 cagtatggag agcatcgaga ttgaccagaa gctgcaggag atcatgaagc agacagggta
      481 cctgactatc gggggccagc gttatcaggc agaaatcaat gacttggaga acttgggtga
      541 gatgggcagt ggtacctgtg gtcaggtgtg gaagatgcgg ttccggaaga cagggcacat
      601 cattgctgtt aagcaaatgc ggcgctctgg gaacaaggaa gagaataagc gcattttgat
      661 ggacctggat gtagtactca agagccatga ctggccttac atcgttcagt gctttgg
//
