LOCUS       sequence-218164-        5382 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targeted A to G in wheat plants or monocotyledons protoplasts .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C
  TITLE     Expanded base editing in rice and wheat using a Cas9-adenosine 
            deaminase fusion.
  JOURNAL   Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.
  PUBMED    29807545
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2018-05-29"; volume: "19(1):59. doi"; pages: " 
            10.1186/s13059-018-1443-z"
COMMENT     Sequence Label: PABE-3
FEATURES             Location/Qualifiers
     source          1..5382
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1198..1218
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1231..1254,1255..1257,1258..5331)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             5244..5252
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5332..5379
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 atgtccgagg tggagttctc tcacgagtat tggatgaggc acgctcttac acttgctaag
       61 agagcttggg acgaaagaga agtgccagtt ggcgccgttc ttgtgcataa taatagggtg
      121 atcggcgagg gttggaatag accaattgga aggcatgatc caacagctca cgcagagatt
      181 atggctctca gacaaggcgg cctcgttatg cagaactaca ggctcattga cgctacactc
      241 tacgtgacac tcgaaccttg cgttatgtgc gccggagcta tgattcattc taggattggc
      301 agggtcgtgt ttggagctag ggacgctaaa acaggagccg ccggatctct tatggacgtg
      361 ttgcatcatc caggcatgaa ccatagggtg gagattacag agggcattct tgcagacgag
      421 tgcgctgctc ttctttccga tttcttcagg atgagaaggc aggagattaa ggcccagaag
      481 aaggctcagt cttctacaga tagcggagga tcttccggag gatctagcgg ctccgagaca
      541 ccaggaacat ccgaaagcgc tacaccagaa tctagcggag gctcttccgg aggatcttct
      601 gaagtggagt tctcccacga gtattggatg aggcacgctc ttacacttgc taaaagggct
      661 agggacgaaa gggaagttcc agttggagct gttctcgtgc tcaataacag ggtgattggc
      721 gagggttgga atagagccat tggactccat gatccaacag ctcacgcaga gattatggct
      781 cttagacaag gcggcctcgt tatgcagaat tacagactca tcgacgccac actctacgtt
      841 accttcgaac cttgcgttat gtgcgccgga gctatgatcc attctaggat tggcagggtc
      901 gtgttcggcg ttagaaacgc taagacagga gctgcaggct ctcttatgga cgttcttcat
      961 tacccaggca tgaatcatag agtggagatc acagaaggca ttcttgcaga cgagtgcgca
     1021 gctctccttt gctatttctt caggatgccg aggcaagttt tcaacgctca gaagaaggcc
     1081 cagtcttcta cagattccgg cggatcttct ggaggatcta gcggctccga gacaccagga
     1141 acatccgaat ccgctacacc agagtcttct ggaggatcta gcggaggatc tcttaagcca
     1201 aaaaagaaga gaaaggtttc aggcggctcc gacaagaagt actcgatcgg cctcgccatt
     1261 gggactaact ctgttggctg ggccgtgatc accgacgagt acaaggtgcc ctcaaagaag
     1321 ttcaaggtcc tgggcaacac cgatcggcat tccatcaaga agaatctcat tggcgctctc
     1381 ctgttcgaca gcggcgagac ggctgaggct acgcggctca agcgcaccgc ccgcaggcgg
     1441 tacacgcgca ggaagaatcg catctgctac ctgcaggaga ttttctccaa cgagatggcg
     1501 aaggttgacg attctttctt ccacaggctg gaggagtcat tcctcgtgga ggaggataag
     1561 aagcacgagc ggcatccaat cttcggcaac attgtcgacg aggttgccta ccacgagaag
     1621 taccctacga tctaccatct gcggaagaag ctcgtggact ccacagataa ggcggacctc
     1681 cgcctgatct acctcgctct ggcccacatg attaagttca ggggccattt cctgatcgag
     1741 ggggatctca acccggacaa tagcgatgtt gacaagctgt tcatccagct cgtgcagacg
     1801 tacaaccagc tcttcgagga gaaccccatt aatgcgtcag gcgtcgacgc gaaggctatc
     1861 ctgtccgcta ggctctcgaa gtctcggcgc ctcgagaacc tgatcgccca gctgccgggc
     1921 gagaagaaga acggcctgtt cgggaatctc attgcgctca gcctggggct cacgcccaac
     1981 ttcaagtcga atttcgatct cgctgaggac gccaagctgc agctctccaa ggacacatac
     2041 gacgatgacc tggataacct cctggcccag atcggcgatc agtacgcgga cctgttcctc
     2101 gctgccaaga atctgtcgga cgccatcctc ctgtctgata ttctcagggt gaacaccgag
     2161 attacgaagg ctccgctctc agcctccatg atcaagcgct acgacgagca ccatcaggat
     2221 ctgaccctcc tgaaggcgct ggtcaggcag cagctccccg agaagtacaa ggagatcttc
     2281 ttcgatcagt cgaagaacgg ctacgctggg tacattgacg gcggggcctc tcaggaggag
     2341 ttctacaagt tcatcaagcc gattctggag aagatggacg gcacggagga gctgctggtg
     2401 aagctcaatc gcgaggacct cctgaggaag cagcggacat tcgataacgg cagcatccca
     2461 caccagattc atctcgggga gctgcacgct atcctgagga ggcaggagga cttctaccct
     2521 ttcctcaagg ataaccgcga gaagatcgag aagattctga ctttcaggat cccgtactac
     2581 gtcggcccac tcgctagggg caactcccgc ttcgcttgga tgacccgcaa gtcagaggag
     2641 acgatcacgc cgtggaactt cgaggaggtg gtcgacaagg gcgctagcgc tcagtcgttc
     2701 atcgagagga tgacgaattt cgacaagaac ctgccaaatg agaaggtgct ccctaagcac
     2761 tcgctcctgt acgagtactt cacagtctac aacgagctga ctaaggtgaa gtatgtgacc
     2821 gagggcatga ggaagccggc tttcctgtct ggggagcaga agaaggccat cgtggacctc
     2881 ctgttcaaga ccaaccggaa ggtcacggtt aagcagctca aggaggacta cttcaagaag
     2941 attgagtgct tcgattcggt cgagatctct ggcgttgagg accgcttcaa cgcctccctg
     3001 gggacctacc acgatctcct gaagatcatt aaggataagg acttcctgga caacgaggag
     3061 aatgaggata tcctcgagga cattgtgctg acactcactc tgttcgagga ccgggagatg
     3121 atcgaggagc gcctgaagac ttacgcccat ctcttcgatg acaaggtcat gaagcagctc
     3181 aagaggagga ggtacaccgg ctgggggagg ctgagcagga agctcatcaa cggcattcgg
     3241 gacaagcagt ccgggaagac gatcctcgac ttcctgaaga gcgatggctt cgcgaaccgc
     3301 aatttcatgc agctgattca cgatgacagc ctcacattca aggaggatat ccagaaggct
     3361 caggtgagcg gccaggggga ctcgctgcac gagcatatcg cgaacctcgc tggctcgcca
     3421 gctatcaaga aggggattct gcagaccgtg aaggttgtgg acgagctggt gaaggtcatg
     3481 ggcaggcaca agcctgagaa catcgtcatt gagatggccc gggagaatca gaccacgcag
     3541 aagggccaga agaactcacg cgagaggatg aagaggatcg aggagggcat taaggagctg
     3601 gggtcccaga tcctcaagga gcacccggtg gagaacacgc agctgcagaa tgagaagctc
     3661 tacctgtact acctccagaa tggccgcgat atgtatgtgg accaggagct ggatattaac
     3721 aggctcagcg attacgacgt cgatcatatc gttccacagt cattcctgaa ggatgactcc
     3781 attgacaaca aggtcctcac caggtcggac aagaaccggg gcaagtctga taatgttcct
     3841 tcagaggagg tcgttaagaa gatgaagaac tactggcgcc agctcctgaa tgccaagctg
     3901 atcacgcagc ggaagttcga taacctcaca aaggctgaga ggggcgggct ctctgagctg
     3961 gacaaggcgg gcttcatcaa gaggcagctg gtcgagacac ggcagatcac taagcacgtt
     4021 gcgcagattc tcgactcacg gatgaacact aagtacgatg agaatgacaa gctgatccgc
     4081 gaggtgaagg tcatcaccct gaagtcaaag ctcgtctccg acttcaggaa ggatttccag
     4141 ttctacaagg ttcgggagat caacaattac caccatgccc atgacgcgta cctgaacgcg
     4201 gtggtcggca cagctctgat caagaagtac ccaaagctcg agagcgagtt cgtgtacggg
     4261 gactacaagg tttacgatgt gaggaagatg atcgccaagt cggagcagga gattggcaag
     4321 gctaccgcca agtacttctt ctactctaac attatgaatt tcttcaagac agagatcact
     4381 ctggccaatg gcgagatccg gaagcgcccc ctcatcgaga cgaacggcga gacgggggag
     4441 atcgtgtggg acaagggcag ggatttcgcg accgtcagga aggttctctc catgccacaa
     4501 gtgaatatcg tcaagaagac agaggtccag actggcgggt tctctaagga gtcaattctg
     4561 cctaagcgga acagcgacaa gctcatcgcc cgcaagaagg actgggatcc gaagaagtac
     4621 ggcgggttcg acagccccac tgtggcctac tcggtcctgg ttgtggcgaa ggttgagaag
     4681 ggcaagtcca agaagctcaa gagcgtgaag gagctgctgg ggatcacgat tatggagcgc
     4741 tccagcttcg agaagaaccc gatcgatttc ctggaggcga agggctacaa ggaggtgaag
     4801 aaggacctga tcattaagct ccccaagtac tcactcttcg agctggagaa cggcaggaag
     4861 cggatgctgg cttccgctgg cgagctgcag aaggggaacg agctggctct gccgtccaag
     4921 tatgtgaact tcctctacct ggcctcccac tacgagaagc tcaagggcag ccccgaggac
     4981 aacgagcaga agcagctgtt cgtcgagcag cacaagcatt acctcgacga gatcattgag
     5041 cagatttccg agttctccaa gcgcgtgatc ctggccgacg cgaatctgga taaggtcctc
     5101 tccgcgtaca acaagcaccg cgacaagcca atcagggagc aggctgagaa tatcattcat
     5161 ctcttcaccc tgacgaacct cggcgcccct gctgctttca agtacttcga cacaactatc
     5221 gatcgcaaga ggtacacaag cactaaggag gtcctggacg cgaccctcat ccaccagtcg
     5281 attaccggcc tctacgagac gcgcatcgac ctgtctcagc tcgggggcga caagcggcca
     5341 gcggcgacga agaaggcggg gcaggcgaag aagaagaagt ag
//
