LOCUS       sequence-21817-e         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Tournier C, Whitmarsh AJ, Cavanagh J, Barrett T, Davis RJ
  TITLE     The MKK7 gene encodes a group of c-Jun NH2-terminal kinase kinases.
  JOURNAL   Mol Cell Biol. 1999 Feb . 19(2):1569-81.
  PUBMED    9891090
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1999 Feb . 19(2):1569-81."
COMMENT     Sequence Label: pCDNA3 Flag MKK7g2
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             93..116
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(291..302)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 agctctctgg ctactagaga accactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttcg gcagccaaca tggactataa ggacgatgat gacaaaatgg
      121 cggcgtcctc cctggagcag aagctgtccc gcctggaagc caagctgaag caggagaacc
      181 gtgaggcccg caggaggatc gacctcaact tggatatcag cccacagcgg cccaggccca
      241 ttattgtgat cactctaagc cctgctcctg ccccgtccca gcgagcagcc ctgcaactcc
      301 cactggccaa cgatgggggc agccgctcac catcctcaga gagctcccca cagcacccta
      361 caccccccac ccggccccgc cacatgctgg ggctcccatc aaccttgttc acaccgcgca
      421 gtatggagag catcgagatt gaccagaagc tgcaggagat catgaagcag acagggtacc
      481 tgactatcgg gggccagcgt tatcaggcag aaatcaatga cttggagaac ttgggtgaga
      541 tgggcagtgg tacctgtggt caggtgtgga agatgcggtt ccggaagaca ggccacatca
      601 ttgctgttaa gcaaatgcgg cgctctggga acaaggaaga gaataagcgc attttgatgg
      661 acctggatgt agtactcaag agccatgact gcccttacat cgttcagtgc tttggcacct
      721 tcatcaccaa cacagacgtc tttattgcca tggagctcat gggcacatgt gcag
//
