LOCUS       sequence-218174-         563 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression esgRNA in rice protoplasts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C
  TITLE     Expanded base editing in rice and wheat using a Cas9-adenosine 
            deaminase fusion.
  JOURNAL   Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.
  PUBMED    29807545
REFERENCE   2  (bases 1 to 563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2018-05-29"; volume: "19(1):59. doi"; pages: " 
            10.1186/s13059-018-1443-z"
COMMENT     Sequence Label: pOsU3-esgRNA
FEATURES             Location/Qualifiers
     source          1..563
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        58..437
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     terminator      544..563
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
ORIGIN
        1 agtaattcat ccaggtcacc aagttctagg attttcagaa ctgcaactta ttttatcaag
       61 gaatctttaa acatacgaac agatcactta aagttcttct gaagcaactt aaagttatca
      121 ggcatgcatg gatcttggag gaatcagatg tgcagtcagg gaccatagca caagacaggc
      181 gtcttctact ggtgctacca gcaaatgctg gaagccggga acactgggta cgttggaaac
      241 cacgtgatgt gaagaagtaa gataaactgt aggagaaaag catttcgtag tgggccatga
      301 agcctttcag gacatgtatt gcagtatggg ccggcccatt acgcaattgg acgacaacaa
      361 agactagtat tagtaccacc tcggctatcc acatagatca aagctgattt aaaagagttg
      421 tgcagatgat ccgtggctga gaccctgcag ggtctctgtt taagagctat gctggaaaca
      481 gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
      541 tgcttttttt gttttttatg tct
//
