LOCUS       sequence-218176-         489 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression esgRNA in wheat protoplasts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C
  TITLE     Expanded base editing in rice and wheat using a Cas9-adenosine 
            deaminase fusion.
  JOURNAL   Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.
  PUBMED    29807545
REFERENCE   2  (bases 1 to 489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2018-05-29"; volume: "19(1):59. doi"; pages: " 
            10.1186/s13059-018-1443-z"
COMMENT     Sequence Label: pTaU6-esgRNA
FEATURES             Location/Qualifiers
     source          1..489
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      470..489
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
ORIGIN
        1 gaccaagccc gttattctga cagttctggt gctcaacaca tttatattta tcaaggagca
       61 cattgttact cactgctagg agggaatcga actaggaata ttgatcagag gaactacgag
      121 agagctgaag ataactgccc tctagctctc actgatctgg gtcgcatagt gagatgcagc
      181 ccacgtgagt tcagcaacgg tctagcgctg ggcttttagg cccgcatgat cgggcttttg
      241 tcgggtggtc gacgtgttca cgattgggga gagcaacgca gcagttcctc ttagtttagt
      301 cccacctcgc ctgtccagca gagttctgac cggtttataa actcgcttgc tgcatcagac
      361 ttgtgagacc ctgcagggtc tctgtttaag agctatgctg gaaacagcat agcaagttta
      421 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttgttt
      481 tttatgtct
//
