LOCUS       sequence-218178-        9332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PPI reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9332)
  AUTHORS   Zhang Q, Zheng YW, Coughlin SR, Shu X
  TITLE     A rapid fluorogenic GPCR-beta-arrestin interaction assay.
  JOURNAL   Protein Sci. 2018 Apr;27(4):874-879. doi: 10.1002/pro.3385. Epub 
            2018 Feb 23.
  PUBMED    29411438
REFERENCE   2  (bases 1 to 9332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.3385"; 
            journalName: "Protein Sci"; date: "2018-04"; volume: "27"; issue: 
            "4"; pages: "874-879"
COMMENT     Sequence Label: PCDNA3-b2AR-GFP11-T2A-GFP10-bArrestin1-T2A mCh...
FEATURES             Location/Qualifiers
     source          1..9332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2114..2167
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2174..2183
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2270..2290
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             2324..2593
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     CDS             3887..3940
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3947..4657
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4092..4110)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4360..4379
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4689..4706)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4695..4919
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4965..5393
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5052..5071)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5262..5283
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5402..5422)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5407..5736
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5587..5722
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5649..5668
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5803..6597
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5857..5876)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6467..6486
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6771..6892
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6808..6827)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6862..6881
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6941..6957)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6941..6957)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6954..6976)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6965..6981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6989..6995,6996..7013,7014..7019))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7034..7055
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7172..7189)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7343..7931)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7423..7442)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8102..8893,8894..8962))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8725..8744
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8963..9067)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9142..9161)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttgccaccat gaagacgatc atcgccctga gctacatctt
      721 ctgcctggtg ttcgccgact acaaggacga tgatgacgcc atggggcaac ccgggaacgg
      781 cagcgccttc ttgctggcac ccaatagaag ccatgcgccg gaccacgacg tcacgcagca
      841 aagggacgag gtgtgggtgg tgggcatggg catcgtcatg tctctcatcg tcctggccat
      901 cgtgtttggc aatgtgctgg tcatcacagc cattgccaag ttcgagcgtc tgcagacggt
      961 caccaactac ttcatcactt cactggcctg tgctgatctg gtcatgggcc tggcagtggt
     1021 gccctttggg gccgcccata ttcttatgaa aatgtggact tttggcaact tctggtgcga
     1081 gttttggact tccattgatg tgctgtgcgt cacggccagc attgagaccc tgtgcgtgat
     1141 cgcagtggat cgctactttg ccattacttc acctttcaag taccagagcc tgctgaccaa
     1201 gaataaggcc cgggtgatca ttctgatggt gtggattgtg tcaggcctta cctccttctt
     1261 gcccattcag atgcactggt atcgggccac ccaccaggaa gccatcaact gctatgccaa
     1321 tgagacctgc tgtgacttct tcacgaacca agcctatgcc attgcctctt ccatcgtgtc
     1381 cttctacgtt cccctggtga tcatggtctt cgtctactcc agggtctttc aggaggccaa
     1441 aaggcagctc cagaagattg acaaatctga gggccgcttc catgtccaga accttagcca
     1501 ggtggagcag gatgggcgga cggggcatgg actccgcaga tcttccaagt tctgcttgaa
     1561 ggagcacaaa gccctcaaga cgttaggcat catcatgggc actttcaccc tctgctggct
     1621 gcccttcttc atcgttaaca ttgtgcatgt gatccaggat aacctcatcc gtaaggaagt
     1681 ttacatcctc ctaaattgga taggctatgt caattctggt ttcaatcccc ttatctactg
     1741 ccggagccca gatttcagga ttgccttcca ggagcttctg tgcctgcgca ggtcttcttt
     1801 gaaggcctat gggaatggct actccagcaa cggcaacaca ggggagcaga gtggatatca
     1861 cgtggaacag gagaaagaaa ataaactgct gtgtgaagac ctcccaggca cggaagactt
     1921 tgtgggccat caaggtactg tgcctagcga taacattgat tcacaaggga ggaattgtag
     1981 tacaaatgac tcactgctgg cattaattaa ctctggtggc gggagtggtg gcgggggtac
     2041 catggaaaag cgagaccata tggttttgct tgagtatgtt acagcggctg gcattaccga
     2101 tgcatcagga tccgagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc
     2161 tggcccagaa ttcgccacca tggacctgcc tgacgaccac tacctgtcca cccagaccat
     2221 cctgtccaag gacctgaact ccggactcag atctggcagc ggtggaggcg aaaatctcta
     2281 cttccagagt agcgcatccg gcggaagcgg aagcctcgag atggagatgt ggcacgaggg
     2341 actcgaagag gccagcaggc tgtactttgg cgagaggaac gtcaagggca tgttcgaagt
     2401 gctggagccc ctccatgcga tgatggaaag gggcccacag accctgaagg agaccagctt
     2461 caaccaggct tacggcaggg acctgatgga ggcacaggaa tggtgcagga agtacatgaa
     2521 gagcggcaac gtgaaagacc tgacccaggc gtgggacctc tactaccacg tgttcaggag
     2581 gatcagcaag cagggcggag gaggcagcgg gcgcgccatg ggcgacaaag ggacacgagt
     2641 gttcaagaag gcaagcccca atggaaagct cactgtctac ctgggaaagc gggactttgt
     2701 ggaccacatt gacctcgtgg accccgtgga tggtgtggtc ctggtggatc ctgagtatct
     2761 caaagaaagg cgagtctacg tgacactgac ctgcgccttc cggtatggcc gggaagacct
     2821 ggatgtcttg ggtctgactt ttcggaaaga cctgtttgtg gccaatgtgc agtccttccc
     2881 accggccccc gaggacaaga agccactgac acggctacag gagcgactca tcaagaagct
     2941 gggcgagcat gcctgcccct tcacctttga gatcccgcca aaccttccat gctcagtcac
     3001 tctgcagcct gggcctgagg acacaggaaa ggcctgtggt gtggattatg aagtcaaagc
     3061 cttctgtgct gagaacctgg aggagaagat ccacaaaagg aactctgtgc ggctagtcat
     3121 ccggaaggtt cagtatgccc ctgagagacc tggccctcag cccacggctg agactaccag
     3181 acagttcctt atgtcagaca agcccctaca ccttgaggca tcactggata aggagatcta
     3241 ttaccacgga gaacccatca gcgttaatgt ccacgtcacc aacaacacca acaagactgt
     3301 gaagaagatc aagatctcgg tgcgccagta tgcagacatc tgtctcttca acacagctca
     3361 gtacaagtgc ccagtggcca tggaggaggc tgatgacaat gtggcaccca gctcaacatt
     3421 ctgcaaggtc tatacgctga ctcccttcct ggccaacaac cgagagaagc ggggactcgc
     3481 cctcgacggg aagctcaagc atgaggacac gaatctggct tccagcacgc tgttgcggga
     3541 aggtgccaac cgtgaaatcc tgggcatcat cgtttcctac aaagtcaagg tgaaactggt
     3601 ggtgtcccgg ggcggcctct tgggagacct tgcatccagt gatgtggccg tggagctgcc
     3661 ttttacccta atgcacccca agcctaagga ggagccccca catcgggaag ttccagagag
     3721 cgagactcca gtagacacca atctcataga gcttgacacc aatgatgacg acattgtatt
     3781 tgaggacttt gctcgccagc ggctgaaagg catgaaggat gacaaggacg aagaggatga
     3841 cggcaccggc tctccgcacc tcaacaacag aggcggccgc tctagagagg gcagaggaag
     3901 tctgctaaca tgcggtgacg tcgaggagaa tcctggccca gaattgatgg tgagcaaggg
     3961 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     4021 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     4081 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     4141 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     4201 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     4261 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     4321 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     4381 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     4441 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     4501 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     4561 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     4621 ccactccacc ggcggcatgg acgagctgta caagtaatct agagggcccg tttaaacccg
     4681 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     4741 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     4801 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     4861 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     4921 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     4981 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5041 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5101 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5161 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5221 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5281 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5341 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     5401 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     5461 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     5521 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     5581 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     5641 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     5701 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     5761 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     5821 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     5881 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     5941 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     6001 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     6061 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     6121 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     6181 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     6241 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     6301 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     6361 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     6421 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     6481 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     6541 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     6601 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     6661 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     6721 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     6781 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6841 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6901 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     6961 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7021 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7081 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7141 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7201 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7261 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7321 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7381 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7441 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7501 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7561 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7621 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7681 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7741 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     7801 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7861 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7921 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7981 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8041 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8101 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8161 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8221 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8281 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8341 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8401 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8461 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8521 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8581 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8641 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8701 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8761 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8821 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8881 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8941 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9001 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9061 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     9121 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     9181 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     9241 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     9301 gcttcgcgat gtacgggcca gatatacgcg tt
//
