LOCUS       sequence-218185-        9272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PPI reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Zhang Q, Zheng YW, Coughlin SR, Shu X
  TITLE     A rapid fluorogenic GPCR-beta-arrestin interaction assay.
  JOURNAL   Protein Sci. 2018 Apr;27(4):874-879. doi: 10.1002/pro.3385. Epub 
            2018 Feb 23.
  PUBMED    29411438
REFERENCE   2  (bases 1 to 9272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.3385"; 
            journalName: "Protein Sci"; date: "2018-04"; volume: "27"; issue: 
            "4"; pages: "874-879"
COMMENT     Sequence Label: PCDNA3-muOR-GFP11-T2A-GFP10-bArrestin1-T2A mCh...
FEATURES             Location/Qualifiers
     source          1..9272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(39..59)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        44..373
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      224..359
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     286..305
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             440..1234
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(494..513)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1104..1123
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1408..1529
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1445..1464)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1499..1518
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1578..1594)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1578..1594)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1591..1613)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1602..1618
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1626..1632,1633..1650,1651..1656))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1671..1692
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1809..1826)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1980..2568)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2060..2079)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2739..3530,3531..3599))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3362..3381
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3600..3704)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3779..3798)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3970..4349
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4350..4553
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4504..4524
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4598..4617
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4598..4616
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6023..6076
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      6083..6092
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6179..6199
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             6233..6502
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     CDS             7796..7849
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7856..8566
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8001..8019)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8269..8288
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(8598..8615)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8604..8828
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(8874..9272,1..30)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8961..8980)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9171..9192
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
       61 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
      121 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
      181 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
      241 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
      301 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
      361 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
      421 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
      481 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
      541 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
      601 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
      661 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
      721 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
      781 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
      841 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
      901 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
      961 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     1021 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     1081 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     1141 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     1201 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     1261 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     1321 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     1381 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     1441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     1501 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     1561 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     1621 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     1681 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     1741 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     1801 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1861 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1921 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1981 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     2041 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     2101 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2161 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2221 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2281 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2341 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2401 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2461 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2521 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2581 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2641 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     2701 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     2761 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     2821 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     2881 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     2941 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3001 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3061 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3121 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     3181 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     3241 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3301 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     3361 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3421 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     3481 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3541 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     3601 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     3661 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     3721 gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta
     3781 caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg
     3841 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
     3901 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
     3961 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     4021 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     4081 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     4141 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     4201 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     4261 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     4321 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     4381 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     4441 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     4501 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
     4561 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
     4621 cccaagctgg ctagcgttta aacttaagct tgccaccatg aagacgatca tcgccctgag
     4681 ctacatcttc tgcctggtgt tcgccgacta caaggacgat gatgacgcca tgggcccagg
     4741 gaacatcagc gactgctctg accccttagc tcctgcaagt tgctccccag cacctggctc
     4801 ctggctcaac ttgtcccacg ttgatggcaa ccagtccgac ccatgcggtc ctaaccgcac
     4861 ggggcttggc gggagccaca gcctgtgccc tcagaccggc agcccttcca tggtcacagc
     4921 catcaccatc atggccctct attctatcgt gtgtgtagtg ggcctctttg gaaacttcct
     4981 ggtcatgtat gtgattgtaa gatataccaa aatgaagact gccaccaaca tctacatttt
     5041 caaccttgct ctggcagatg ccttagccac tagcacgctg ccctttcaga gtgttaacta
     5101 cctgatggga acgtggccct ttggaaacat cctctgcaag atcgtgatct caatagacta
     5161 ctacaacatg ttcaccagta tcttcaccct ctgcaccatg agtgtagacc gctacattgc
     5221 cgtctgccac ccggtcaagg ccctggattt ccgtaccccc cgaaatgcca aaattgtcaa
     5281 tgtctgcaac tggatcctct cttctgccat tggtctgccc gtaatgttca tggcaaccac
     5341 aaaatacagg caggggtcca tagattgcac cctcacgttc tctcatccca catggtactg
     5401 ggagaacctg ctcaaaatct gtgtcttcat cttcgccttc atcatgccgg tcctcatcat
     5461 cactgtgtgt tatggactga tgatcttacg actcaagagt gtccgcatgc tgtcgggctc
     5521 caaagaaaag gacaggaacc tgcgcaggat cacccggatg gtgctggtgg tcgtggctgt
     5581 atttattgtc tgctggaccc ccatccacat ctatgtcatc atcaaagcac tgatcacgat
     5641 tccagaaacc actttccaga ctgtttcctg gcacttctgc attgccttgg gttacacaaa
     5701 cagctgcctg aacccagttc tttatgcgtt cctggatgaa aacttcaaac gatgttttag
     5761 agagttctgc atcccaactt cctccacaat cgaacagcaa aactctgctc gaatccgtca
     5821 aaacactagg gaacacccct ccacggctaa tacagtggat cgaactaacc accagctaga
     5881 aaatctggaa gcagaaactg ctccattgcc cttaattaac tctggtggcg ggagtggtgg
     5941 cgggggtacc atggaaaagc gagaccatat ggttttgctt gagtatgtta cagcggctgg
     6001 cattaccgat gcatcaggat ccgagggcag aggaagtctg ctaacatgcg gtgacgtcga
     6061 ggagaatcct ggcccagaat tcgccaccat ggacctgcct gacgaccact acctgtccac
     6121 ccagaccatc ctgtccaagg acctgaactc cggactcaga tctggcagcg gtggaggcga
     6181 aaatctctac ttccagagta gcgcatccgg cggaagcgga agcctcgaga tggagatgtg
     6241 gcacgaggga ctcgaagagg ccagcaggct gtactttggc gagaggaacg tcaagggcat
     6301 gttcgaagtg ctggagcccc tccatgcgat gatggaaagg ggcccacaga ccctgaagga
     6361 gaccagcttc aaccaggctt acggcaggga cctgatggag gcacaggaat ggtgcaggaa
     6421 gtacatgaag agcggcaacg tgaaagacct gacccaggcg tgggacctct actaccacgt
     6481 gttcaggagg atcagcaagc agggcggagg aggcagcggg cgcgccatgg gcgacaaagg
     6541 gacacgagtg ttcaagaagg caagccccaa tggaaagctc actgtctacc tgggaaagcg
     6601 ggactttgtg gaccacattg acctcgtgga ccccgtggat ggtgtggtcc tggtggatcc
     6661 tgagtatctc aaagaaaggc gagtctacgt gacactgacc tgcgccttcc ggtatggccg
     6721 ggaagacctg gatgtcttgg gtctgacttt tcggaaagac ctgtttgtgg ccaatgtgca
     6781 gtccttccca ccggcccccg aggacaagaa gccactgaca cggctacagg agcgactcat
     6841 caagaagctg ggcgagcatg cctgcccctt cacctttgag atcccgccaa accttccatg
     6901 ctcagtcact ctgcagcctg ggcctgagga cacaggaaag gcctgtggtg tggattatga
     6961 agtcaaagcc ttctgtgctg agaacctgga ggagaagatc cacaaaagga actctgtgcg
     7021 gctagtcatc cggaaggttc agtatgcccc tgagagacct ggccctcagc ccacggctga
     7081 gactaccaga cagttcctta tgtcagacaa gcccctacac cttgaggcat cactggataa
     7141 ggagatctat taccacggag aacccatcag cgttaatgtc cacgtcacca acaacaccaa
     7201 caagactgtg aagaagatca agatctcggt gcgccagtat gcagacatct gtctcttcaa
     7261 cacagctcag tacaagtgcc cagtggccat ggaggaggct gatgacaatg tggcacccag
     7321 ctcaacattc tgcaaggtct atacgctgac tcccttcctg gccaacaacc gagagaagcg
     7381 gggactcgcc ctcgacggga agctcaagca tgaggacacg aatctggctt ccagcacgct
     7441 gttgcgggaa ggtgccaacc gtgaaatcct gggcatcatc gtttcctaca aagtcaaggt
     7501 gaaactggtg gtgtcccggg gcggcctctt gggagacctt gcatccagtg atgtggccgt
     7561 ggagctgcct tttaccctaa tgcaccccaa gcctaaggag gagcccccac atcgggaagt
     7621 tccagagagc gagactccag tagacaccaa tctcatagag cttgacacca atgatgacga
     7681 cattgtattt gaggactttg ctcgccagcg gctgaaaggc atgaaggatg acaaggacga
     7741 agaggatgac ggcaccggct ctccgcacct caacaacaga ggcggccgct ctagagaggg
     7801 cagaggaagt ctgctaacat gcggtgacgt cgaggagaat cctggcccag aattgatggt
     7861 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     7921 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     7981 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     8041 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     8101 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     8161 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     8221 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     8281 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     8341 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     8401 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     8461 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     8521 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaatcta gagggcccgt
     8581 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     8641 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     8701 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     8761 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     8821 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     8881 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     8941 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     9001 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     9061 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     9121 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     9181 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     9241 tttgccgatt tcggcctatt ggttaaaaaa tg
//
