LOCUS       sequence-218199-        9344 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PPI reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9344)
  AUTHORS   Zhang Q, Zheng YW, Coughlin SR, Shu X
  TITLE     A rapid fluorogenic GPCR-beta-arrestin interaction assay.
  JOURNAL   Protein Sci. 2018 Apr;27(4):874-879. doi: 10.1002/pro.3385. Epub 
            2018 Feb 23.
  PUBMED    29411438
REFERENCE   2  (bases 1 to 9344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.3385"; 
            journalName: "Protein Sci"; date: "2018-04"; volume: "27"; issue: 
            "4"; pages: "874-879"
COMMENT     Sequence Label: PCDNA3-PAR1-GFP11-T2A-GFP10-bArrestin1-T2A mCh...
FEATURES             Location/Qualifiers
     source          1..9344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     249..268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        249..267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      303..312
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1746..1799
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1806..1815
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1902..1922
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1956..2225
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     CDS             3519..3572
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3579..4289
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3724..3742)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3992..4011
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4321..4338)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4327..4551
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4597..5025
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4684..4703)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4894..4915
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5034..5054)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5039..5368
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5219..5354
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5435..6229
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5489..5508)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6099..6118
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6403..6524
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6440..6459)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6494..6513
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6573..6589)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6573..6589)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6586..6608)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6597..6613
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6621..6627,6628..6645,6646..6651))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6666..6687
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6804..6821)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6975..7563)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7055..7074)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7734..8525,8526..8594))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8357..8376
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8595..8699)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8774..8793)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8965..9344
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      241 atcgaaatta atacgactca ctatagggag acccaagctg gctagcgttt aaacttaagc
      301 ttgccaccat ggactacaag gacgatgatg acgccatgat cgatatgggg ccccggcgct
      361 tgctgatcgt cgccctcggc ctcagcctgt gcggtccctt gctgtcttcc cgcgtcccta
      421 tgagccagcc agaatcagag aggacagatg ctacggtgaa cccccgctca ttctttctaa
      481 ggaatcccag tgaaaataca tttgaactgg tccccctggg ggatgaggag gaggaggaga
      541 aaaatgaaag cgtcctgctg gagggtaggg cagtctactt aaatataagc ctccctcctc
      601 acacgccgcc tcctcccttc atctccgagg acgcctccgg atatctgacc agcccctggc
      661 tgacgctctt catgccctcc gtgtacacga ttgtgttcat tgtcagcctt cctctgaacg
      721 tcctggccat cgcagtgttc gtcttgagga tgaaggtcaa gaagccggcc gtggtgtaca
      781 tgctgcacct ggccatggcc gacgtgctct tcgtgtcggt gctccccttc aagatcagct
      841 actacttctc cggcactgat tggcagttcg ggtctggaat gtgccgcttc gccaccgcag
      901 cgttttacgg gaacatgtac gcctccatca tgctcatgac ggtcataagc attgaccggt
      961 tcctggcggt ggtgtatccg atccagtccc tgtcctggcg cactctgggc cgagccaact
     1021 tcacttgcgt ggtcatttgg gtgatggcca tcatgggggt ggtgcccctt ctcctcaagg
     1081 agcagaccac ccgagttccg ggactcaaca tcaccacctg ccacgacgtc ctcagtgaga
     1141 acctgatgca aggcttttac tcgtactact tctcggcctt ctccgccatc ttctttcttg
     1201 tgccgttgat cgtttccacg gtctgctaca cgtccatcat ccggtgcctg agctcctccg
     1261 cggttgccaa ccggagcaag aagtcgcggg ctttgttcct gtctgccgcg gtgttctgca
     1321 tcttcatcgt ctgctttggg cccaccaacg tcctcctgat tgtgcactac cttttcctct
     1381 ccgacagtcc tggtacagag gcagcctact ttgcttacct cctctgcgtc tgtgtgagca
     1441 gcgtgagctg ctgcatcgat ccgttgattt actactacgc ctcctccgag tgccagaggc
     1501 acctctacag catcttgtgc tgcaaagaaa gctctgatcc caacagttgc aacagcaccg
     1561 gccagctgat gccgagtaaa atggatacct gctctagtca cctgaataac agcatataca
     1621 aaaagctatt agctttaatt aactctggtg gcgggagtgg tggcgggggt accatggaaa
     1681 agcgagacca tatggttttg cttgagtatg ttacagcggc tggcattacc gatgcatcag
     1741 gatccgaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat cctggcccag
     1801 aattcgccac catggacctg cctgacgacc actacctgtc cacccagacc atcctgtcca
     1861 aggacctgaa ctccggactc agatctggca gcggtggagg cgaaaatctc tacttccaga
     1921 gtagcgcatc cggcggaagc ggaagcctcg agatggagat gtggcacgag ggactcgaag
     1981 aggccagcag gctgtacttt ggcgagagga acgtcaaggg catgttcgaa gtgctggagc
     2041 ccctccatgc gatgatggaa aggggcccac agaccctgaa ggagaccagc ttcaaccagg
     2101 cttacggcag ggacctgatg gaggcacagg aatggtgcag gaagtacatg aagagcggca
     2161 acgtgaaaga cctgacccag gcgtgggacc tctactacca cgtgttcagg aggatcagca
     2221 agcagggcgg aggaggcagc gggcgcgcca tgggcgacaa agggacacga gtgttcaaga
     2281 aggcaagccc caatggaaag ctcactgtct acctgggaaa gcgggacttt gtggaccaca
     2341 ttgacctcgt ggaccccgtg gatggtgtgg tcctggtgga tcctgagtat ctcaaagaaa
     2401 ggcgagtcta cgtgacactg acctgcgcct tccggtatgg ccgggaagac ctggatgtct
     2461 tgggtctgac ttttcggaaa gacctgtttg tggccaatgt gcagtccttc ccaccggccc
     2521 ccgaggacaa gaagccactg acacggctac aggagcgact catcaagaag ctgggcgagc
     2581 atgcctgccc cttcaccttt gagatcccgc caaaccttcc atgctcagtc actctgcagc
     2641 ctgggcctga ggacacagga aaggcctgtg gtgtggatta tgaagtcaaa gccttctgtg
     2701 ctgagaacct ggaggagaag atccacaaaa ggaactctgt gcggctagtc atccggaagg
     2761 ttcagtatgc ccctgagaga cctggccctc agcccacggc tgagactacc agacagttcc
     2821 ttatgtcaga caagccccta caccttgagg catcactgga taaggagatc tattaccacg
     2881 gagaacccat cagcgttaat gtccacgtca ccaacaacac caacaagact gtgaagaaga
     2941 tcaagatctc ggtgcgccag tatgcagaca tctgtctctt caacacagct cagtacaagt
     3001 gcccagtggc catggaggag gctgatgaca atgtggcacc cagctcaaca ttctgcaagg
     3061 tctatacgct gactcccttc ctggccaaca accgagagaa gcggggactc gccctcgacg
     3121 ggaagctcaa gcatgaggac acgaatctgg cttccagcac gctgttgcgg gaaggtgcca
     3181 accgtgaaat cctgggcatc atcgtttcct acaaagtcaa ggtgaaactg gtggtgtccc
     3241 ggggcggcct cttgggagac cttgcatcca gtgatgtggc cgtggagctg ccttttaccc
     3301 taatgcaccc caagcctaag gaggagcccc cacatcggga agttccagag agcgagactc
     3361 cagtagacac caatctcata gagcttgaca ccaatgatga cgacattgta tttgaggact
     3421 ttgctcgcca gcggctgaaa ggcatgaagg atgacaagga cgaagaggat gacggcaccg
     3481 gctctccgca cctcaacaac agaggcggcc gctctagaga gggcagagga agtctgctaa
     3541 catgcggtga cgtcgaggag aatcctggcc cagaattgat ggtgagcaag ggcgaggagg
     3601 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
     3661 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     3721 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
     3781 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
     3841 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
     3901 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     3961 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
     4021 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
     4081 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     4141 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
     4201 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
     4261 ccggcggcat ggacgagctg tacaagtaat ctagagggcc cgtttaaacc cgctgatcag
     4321 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     4381 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     4441 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     4501 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     4561 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     4621 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4681 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4741 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4801 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4861 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4921 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     4981 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     5041 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5101 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     5161 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     5221 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5281 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     5341 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     5401 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     5461 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     5521 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     5581 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     5641 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     5701 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     5761 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     5821 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     5881 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     5941 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     6001 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     6061 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     6121 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     6181 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     6241 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     6301 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     6361 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     6421 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6481 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     6541 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6601 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6661 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6721 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6781 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6841 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6901 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6961 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7021 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7081 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7141 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7201 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7261 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7321 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7381 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7441 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7501 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7561 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7621 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7681 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7741 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7801 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7861 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7921 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7981 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8041 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8101 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8161 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8221 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8281 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8341 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8401 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8461 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8521 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8581 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8641 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8701 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     8761 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     8821 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     8881 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     8941 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     9001 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     9061 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     9121 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     9181 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     9241 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     9301 ctacttggca gtacatctac gtattagtca tcgctattac catg
//
