LOCUS       sequence-218200-        9215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PPI reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Zhang Q, Zheng YW, Coughlin SR, Shu X
  TITLE     A rapid fluorogenic GPCR-beta-arrestin interaction assay.
  JOURNAL   Protein Sci. 2018 Apr;27(4):874-879. doi: 10.1002/pro.3385. Epub 
            2018 Feb 23.
  PUBMED    29411438
REFERENCE   2  (bases 1 to 9215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.3385"; 
            journalName: "Protein Sci"; date: "2018-04"; volume: "27"; issue: 
            "4"; pages: "874-879"
COMMENT     Sequence Label: PCDNA3-PAR2-GFP11-T2A-GFP10-bArrestin1-T2A mCh...
FEATURES             Location/Qualifiers
     source          1..9215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1225..1278
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1285..1294
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1381..1401
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1435..1704
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
     CDS             2998..3051
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3058..3768
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3203..3221)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3471..3490
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(3800..3817)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3806..4030
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4076..4504
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4163..4182)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4373..4394
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4513..4533)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4518..4847
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4698..4833
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4760..4779
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4914..5708
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4968..4987)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5578..5597
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5882..6003
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5919..5938)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5973..5992
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6052..6068)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6052..6068)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6065..6087)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6076..6092
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6100..6106,6107..6124,6125..6130))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6145..6166
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6283..6300)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6454..7042)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6534..6553)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7213..8004,8005..8073))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7836..7855
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8074..8178)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8253..8272)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8444..8823
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8824..9027
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8978..8998
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9072..9091
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9072..9090
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gatgatgatg acaacaacag taaaggaaga agtcttattg gcagattaga aacccagcct
       61 ccaatcactg ggaaaggggt tccggtagaa cccgggtttt ccatcgacga gttctctgcg
      121 tccatcctca ccgggaagct gaccacggtc tttcttccgg tcgtctacat tattgtgttt
      181 gtgattggtt tgcccagtaa tggcatggcc ctctggatct tccttttccg aacgaagaag
      241 aaacaccccg ccgtgattta catggccaac ctggccttgg ccgacctcct ctctgtcatc
      301 tggttccccc tgaagatctc ctaccaccta catggcaaca actgggtcta cggggaggcc
      361 ctgtgcaagg tgctcattgg ctttttctat ggtaatatgt attgctccat cctcttcatg
      421 acctgcctca gcgtgcagag gtactgggtg atcgtgaacc ccatgggaca ccccaggaag
      481 aaggcaaaca tcgccgttgg cgtctccttg gcaatctggc tattaatttt tctggtcacc
      541 atccctttgt atgtcatgaa gcagaccatc tacattccag cattgaacat caccacctgt
      601 cacgatgtgc tgcctgagga ggtattggtg ggggacatgt tcaattactt cctctcactg
      661 gccattggag tcttcctgtt cccggccctc cttactgcta gcgcctacgt gctcatgatc
      721 aagacgctcc gctcttctgc tatggatgaa cactcagaga agaaaaggca gagggctatc
      781 cgactcatca tcaccgtgct ggccatgtac ttcatctgct ttgctcctag caaccttctg
      841 ctcgtagtgc attatttcct aatcaaaacc cagaggcaga gccacgtcta cgccctctac
      901 cttgtcgccc tctgcctgtc gaccctcaac agctgcatag acccctttgt ctattacttt
      961 gtctcaaaag atttcaggga tcacgccaga aacgcgctcc tctgccgaag tgtccgcact
     1021 gtgaatcgca tgcaaatctc gctcagctcc aacaagttct ccaggaagtc cggctcctac
     1081 tcttcaagct caaccagtgt taaaacctcc tacttaatta actctggtgg cgggagtggt
     1141 ggcgggggta ccatggaaaa gcgagaccat atggttttgc ttgagtatgt tacagcggct
     1201 ggcattaccg atgcatcagg atccgagggc agaggaagtc tgctaacatg cggtgacgtc
     1261 gaggagaatc ctggcccaga attcgccacc atggacctgc ctgacgacca ctacctgtcc
     1321 acccagacca tcctgtccaa ggacctgaac tccggactca gatctggcag cggtggaggc
     1381 gaaaatctct acttccagag tagcgcatcc ggcggaagcg gaagcctcga gatggagatg
     1441 tggcacgagg gactcgaaga ggccagcagg ctgtactttg gcgagaggaa cgtcaagggc
     1501 atgttcgaag tgctggagcc cctccatgcg atgatggaaa ggggcccaca gaccctgaag
     1561 gagaccagct tcaaccaggc ttacggcagg gacctgatgg aggcacagga atggtgcagg
     1621 aagtacatga agagcggcaa cgtgaaagac ctgacccagg cgtgggacct ctactaccac
     1681 gtgttcagga ggatcagcaa gcagggcgga ggaggcagcg ggcgcgccat gggcgacaaa
     1741 gggacacgag tgttcaagaa ggcaagcccc aatggaaagc tcactgtcta cctgggaaag
     1801 cgggactttg tggaccacat tgacctcgtg gaccccgtgg atggtgtggt cctggtggat
     1861 cctgagtatc tcaaagaaag gcgagtctac gtgacactga cctgcgcctt ccggtatggc
     1921 cgggaagacc tggatgtctt gggtctgact tttcggaaag acctgtttgt ggccaatgtg
     1981 cagtccttcc caccggcccc cgaggacaag aagccactga cacggctaca ggagcgactc
     2041 atcaagaagc tgggcgagca tgcctgcccc ttcacctttg agatcccgcc aaaccttcca
     2101 tgctcagtca ctctgcagcc tgggcctgag gacacaggaa aggcctgtgg tgtggattat
     2161 gaagtcaaag ccttctgtgc tgagaacctg gaggagaaga tccacaaaag gaactctgtg
     2221 cggctagtca tccggaaggt tcagtatgcc cctgagagac ctggccctca gcccacggct
     2281 gagactacca gacagttcct tatgtcagac aagcccctac accttgaggc atcactggat
     2341 aaggagatct attaccacgg agaacccatc agcgttaatg tccacgtcac caacaacacc
     2401 aacaagactg tgaagaagat caagatctcg gtgcgccagt atgcagacat ctgtctcttc
     2461 aacacagctc agtacaagtg cccagtggcc atggaggagg ctgatgacaa tgtggcaccc
     2521 agctcaacat tctgcaaggt ctatacgctg actcccttcc tggccaacaa ccgagagaag
     2581 cggggactcg ccctcgacgg gaagctcaag catgaggaca cgaatctggc ttccagcacg
     2641 ctgttgcggg aaggtgccaa ccgtgaaatc ctgggcatca tcgtttccta caaagtcaag
     2701 gtgaaactgg tggtgtcccg gggcggcctc ttgggagacc ttgcatccag tgatgtggcc
     2761 gtggagctgc cttttaccct aatgcacccc aagcctaagg aggagccccc acatcgggaa
     2821 gttccagaga gcgagactcc agtagacacc aatctcatag agcttgacac caatgatgac
     2881 gacattgtat ttgaggactt tgctcgccag cggctgaaag gcatgaagga tgacaaggac
     2941 gaagaggatg acggcaccgg ctctccgcac ctcaacaaca gaggcggccg ctctagagag
     3001 ggcagaggaa gtctgctaac atgcggtgac gtcgaggaga atcctggccc agaattgatg
     3061 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     3121 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     3181 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     3241 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     3301 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     3361 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     3421 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     3481 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     3541 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     3601 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     3661 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     3721 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaatc tagagggccc
     3781 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     3841 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     3901 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     3961 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     4021 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     4081 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4141 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4201 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4261 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4321 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4381 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4441 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     4501 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     4561 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     4621 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     4681 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4741 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     4801 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     4861 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     4921 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     4981 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     5041 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     5101 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     5161 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     5221 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     5281 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     5341 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     5401 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     5461 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     5521 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     5581 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     5641 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     5701 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     5761 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     5821 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     5881 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5941 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6001 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     6061 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     6121 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     6181 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     6241 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6301 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6361 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6421 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6481 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6541 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6601 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6661 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6721 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6781 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6841 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     6901 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6961 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7021 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7081 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7141 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7201 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7261 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7321 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7381 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7441 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7501 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7561 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7621 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7681 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7741 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7801 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7861 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7921 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7981 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8041 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8101 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8161 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     8221 agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     8281 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     8341 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     8401 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
     8461 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     8521 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     8581 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     8641 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     8701 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     8761 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     8821 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     8881 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     8941 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     9001 cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac tgcttactgg
     9061 cttatcgaaa ttaatacgac tcactatagg gagacccaag ctggctagcg tttaaactta
     9121 agcttgccac catgcgaagt ctcagcctgg cgtggctgct gggaggtatc acccttctgg
     9181 cggcctcggt ctcctgcagc cggactgatt acaaa
//
