LOCUS       sequence-218206-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pPSP2 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK026
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(834..868)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
       61 tttcctgcgt tatcccctga ttctgtggat aaccgtagtc ggtctcaaac gtgacccaac
      121 atcagatgac ccaaggtcca cctcttatta aaggacgttt gatccttcga caccatggct
      181 ctgttgaact tttatctgag agaggaaaaa aaggaaggaa aaaaaagaag aaacttcctt
      241 tatttatttg tcttaaccac aacacacaat gcaataagat gcaatataat atcaaagcca
      301 atatcttatg ttgctgatcc tgagaaggaa tatatacaat ttatgtagta aaataccttt
      361 tcttctgcga gttgcaagaa atagaaaaga ctccgattgc gcatcgccag aataaaattt
      421 cacaaccaca ctttttggct gaacttttta ttacctgatt aaacagagag agaaaaggta
      481 gaggtcaaaa ttttttaagc aaaactaaaa aagatgcaaa atcacgtgct gaaaatctaa
      541 cataagggtt aagattagag ttttatagga cttgttttgt aatatttcaa atacgagcta
      601 accctactga tttcaattag gtctaattta gggttgagct gcactgaaat ttcggaaatt
      661 ttgggttatt ttaaatgaga cagaagaact acagagatac gttcttcaga ctttaaagct
      721 tatctccaca aagaattggt caagaaatca tcctagaaaa acacgtttgc tcactcgatc
      781 ttaatcacat agagtgctgg aacgggaaga aagatctatg tgagaccaga ccaataaaaa
      841 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
      901 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca
      961 tcgcagtact gttgtaattc attaagcatt ctgcc
//
