LOCUS       sequence-218210-        8605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the fluorescent protein TagBFP in a lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8605)
  TITLE     Fluorescent protein expressing plasmids suitable for lentiviral 
            approaches
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti6.2_TagBFP
FEATURES             Location/Qualifiers
     source          1..8605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..108
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(109..177,178..969)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(327..346)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1140..1728
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1629..1648
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1882..1899
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2016..2037
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2052..2057,2058..2075,2076..2082)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2090..2106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2095..2117
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2114..2130
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2114..2130
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2149..2169
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2151..2169
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2197..2423
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2424..2604
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2651..2776
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3269..3502
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3687..3731
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3998..4114
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4137..4440
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4441..4644
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4595..4615
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4641..4665
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4744..5445
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    5461..6049
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5514..5534)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5932..5943)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6063..6562
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6098..6121)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6495..6514
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6583..7182
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6583..6602)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7079..7099
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7320..7553
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7625..7746
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7662..7681)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7716..7735
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7786..7921
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7941..7960)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7942..7960)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7970..7987)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7970..7986)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7979..8001)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8128..8583
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8215..8234)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8425..8446
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
       61 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      121 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      181 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      241 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      301 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      361 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      421 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      481 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
      541 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
      601 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
      661 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
      721 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
      781 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
      841 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
      901 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
      961 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1021 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1081 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1141 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1201 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1261 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     1321 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1381 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1441 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1501 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1561 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1621 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1681 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1741 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1801 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     1861 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     1921 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1981 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     2041 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     2101 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     2161 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     2221 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     2281 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     2341 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     2401 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     2461 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     2521 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     2581 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     2641 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     2701 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     2761 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     2821 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     2881 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     2941 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     3001 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     3061 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     3121 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     3181 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     3241 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     3301 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     3361 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     3421 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     3481 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     3541 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     3601 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     3661 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     3721 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     3781 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     3841 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     3901 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     3961 ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa ggggggattg
     4021 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     4081 aattacaaaa acaaattaca aaattcaaaa ttttatcgat aagcttggga gttccgcgtt
     4141 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     4201 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     4261 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     4321 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     4381 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     4441 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     4501 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     4561 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     4621 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     4681 ccacgctgtt ttgacctcca tagaagacac cgactctaga gatatcttcg aaggcgcgcc
     4741 accatgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     4801 gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     4861 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     4921 actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc
     4981 ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
     5041 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     5101 aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc
     5161 tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac
     5221 atggccctga agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga
     5281 tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg
     5341 gaaagaatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc
     5401 agatactgcg acctccctag caaactgggg cacaagctta attaaagcgg ccgcgtcgac
     5461 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     5521 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     5581 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     5641 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     5701 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     5761 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     5821 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     5881 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     5941 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     6001 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcta
     6061 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
     6121 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
     6181 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
     6241 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
     6301 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
     6361 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
     6421 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc
     6481 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
     6541 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc
     6601 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     6661 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     6721 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     6781 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     6841 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     6901 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     6961 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     7021 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     7081 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     7141 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     7201 cagcgcccga ccgaaaggag cgcacgaccc catgcatctc gagggcccgg tacctttaag
     7261 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     7321 ggaagggcta gctcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     7381 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7441 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7501 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     7561 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     7621 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7681 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7741 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     7801 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7861 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7921 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     7981 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     8041 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     8101 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     8161 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     8221 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     8281 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     8341 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     8401 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     8461 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     8521 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     8581 tttaggtggc acttttcggg gaaat
//
