LOCUS       sequence-218212-        7901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of CD44 variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7901)
  TITLE     Georg Weber Lab Plasmids
REFERENCE   2  (bases 1 to 7901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBabe-CD44v6
FEATURES             Location/Qualifiers
     source          1..7901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    257..614
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             679..1095
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1025..1047
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1063..1079
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1105..1479
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1417..1433
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     misc_feature    join(3108..3682,3683..3685,3686..3694)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3275..3292)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3502..3521
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3695..3697,3698..3700,3701..4414)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3695..3697,3698..3700,3701..4411)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3740..3761)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4001..4020)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4348..4369
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             4600..5069
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5199..5216)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5370..5958)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5450..5469)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(6082..6150,6151..6942)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6300..6319)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     LTR             join(7624..7901,1..192)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct
       61 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
      121 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
      181 gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga
      241 cccaccaccg ggaggtaagc tggccagcaa cttatctgtg tctgtccgat tgtctagtgt
      301 ctatgactga ttttatgcgc ctgcgtcggt actagttagc taactagctc tgtatctggc
      361 ggacccgtgg tggaactgac gagttcggaa cacccggccg caaccctggg agacgtccca
      421 gggacttcgg gggccgtttt tgtggcccga cctgagtcca aaaatcccga tcgttttgga
      481 ctctttggtg cacccccctt agaggaggga tatgtggttc tggtaggaga cgagaaccta
      541 aaacagttcc cgcctccgtc tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg
      601 cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg
      661 tctgaaaata tgggcccggg ccagactgtt accactccct taagtttgac cttaggtcac
      721 tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa gagacgttgg
      781 gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg agacggcacc
      841 tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg cccgcatgga
      901 cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga cccccctccc
      961 tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc cgccccgtct
     1021 ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact
     1081 ccttctctag gcgcccccat atggccatat gagatcttat atggggcacc cccgcccctt
     1141 gtaaacttcc ctgaccctga catgacaaga gttactaaca gcccctctct ccaagctcac
     1201 ttacaggctc tctacttagt ccagcacgaa gtctggagac ctctggcggc agcctaccaa
     1261 gaacaactgg accgaccggt ggtacctcac ccttaccgag tcggcgacac agtgtgggtc
     1321 cgccgacacc agactaagaa cctagaacct cgctggaaag gaccttacac agtcctgctg
     1381 accaccccca ccgccctcaa agtagacggc atcgcagctt ggatacacgc cgcccacgtg
     1441 aaggctgccg accccggggg tggaccatcc tctagactgc cggatccatt taaattcgaa
     1501 ttcctgcagg cctcgagggc cggcgcgccg cggtggcggc cgctctagcc cctcgctcgc
     1561 ccacgccatg gacaagtttt ggtggcacat agcttgggga ctttgcctct tgcagttgag
     1621 cctggcacat cagcagatcg atttgaatgt aacctgccgc tacgcaggtg tattccatgt
     1681 ggagaaaaat ggccgctaca gtatctcccg gactgaggca gctgacctct gccaggcttt
     1741 caacagtacc ttacccacca tggaccaaat gaagttggcc ctgagcaagg gttttgaaac
     1801 atgcaggtat gggttcatag aaggaaatgt ggtaattccg aggattcatc ccaacgctat
     1861 ctgtgcagcc aaccacacag gagtatatat cctcgtcacg tccaacacct cccactatga
     1921 cacatattgc ttcaatgcct cagcccctcc tgaagaagac tgtacatcag tcacagacct
     1981 acccaattcc ttcgatggac ccgttaccat aactattgtc aaccgtgatg gtacacgcta
     2041 cagcaagaag ggcgagtata gaacacacca agaagacatc gatgcttcaa acattataga
     2101 tgacgatgtc agcagcggct ccaccatcga gaagagcacc ccagaaggct acattttgca
     2161 cacctacctt cctactgaac agcctactgg agatcaggat gactccttct ttatccggag
     2221 caccttggcc accactccta atagtacagc agaagcagca gctacccagc aggagacgtg
     2281 gtttcagaac ggatggcagg ggaagaaccc ccctacccca agcgaagact cccatgtgac
     2341 agaagggaca actgatacag actccagtca tagtacaacc cttcagccta ctgcagctcc
     2401 aaatacccat ttggtggaag acttgaacag gacaggacca ctttcagtga caactccaca
     2461 gagtcattct cagaacttct ctacattaca tggagagccg gaagaagacg aaaactatcc
     2521 aacaacttct attctgccat ctagcatgac ccctgctaaa actgaggtct ttggagaaac
     2581 tgaagtgact cttgctactg actccaacgt taatgttgat ggctccttac caggagatcg
     2641 agactcatcc aaggactcca gggggagttc ccgcactgtg actcatggat ccgaattagc
     2701 tggacactca agtgcgaacc aggacagtgg agtgaccaca acttctggtc ctatgaggag
     2761 acctcagatt ccagaatggc tcatcatctt ggcatctctc ctggcactgg ctctgattct
     2821 tgccgtctgc atcgcggtca atagtaggag aaggtgtggg cagaagaaaa agctggtgat
     2881 caacggtggc aatgggacag tggaagacag gaaacccagt gagctcaacg gggaggccag
     2941 caagtctcag gaaatggtgc atttggtgaa caaggaacca tcagagaccc cagaccagtg
     3001 tatgacagct gacgagaccc ggaatctgca gagtgtggac atgaagattg gggtgtagtg
     3061 cctgggccga accaggggcg gatcccccgt aaattccgcc cccccccccc ctctccctcc
     3121 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     3181 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     3241 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     3301 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     3361 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     3421 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     3481 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     3541 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     3601 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     3661 ctttgaaaaa cacgatgata atatggccac aaccatggtg agcaagggcg aggagctgtt
     3721 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     3781 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     3841 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     3901 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     3961 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     4021 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     4081 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     4141 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     4201 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     4261 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     4321 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     4381 gatcactctc ggcatggacg agctgtacaa gtaaagcgcc agcacagtgg tcgacgataa
     4441 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
     4501 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa
     4561 tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga
     4621 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat
     4681 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     4741 atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag
     4801 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
     4861 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
     4921 ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc
     4981 ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg
     5041 attgactacc cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt aatttggttt
     5101 tttttcttaa gtatttacat taaatggcca tagttgcatt aatgaatcgg ccaacgcgcg
     5161 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     5221 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     5281 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     5341 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     5401 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     5461 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     5521 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     5581 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     5641 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     5701 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     5761 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     5821 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     5881 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5941 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6001 aacgaaaact cacgttaagg gattttggtc atgagacaat aaccctgata aatgcttcaa
     6061 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6121 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6181 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6241 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6301 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6361 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6421 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     6481 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     6541 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     6601 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6661 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6721 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6781 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6841 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6901 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6961 tcatatatac tttagattga tttgcggccg gccgcaaaag gatctaggtg aagatccttt
     7021 ttgataatct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7081 ttccgcgcac atttccccga aaagtgccac cagctttgct cttaggagtt tcctaataca
     7141 tcccaaactc aaatatataa agcatttgac ttgttctatg ccctaggggg cggggggaag
     7201 ctaagccagc tttttttaac atttaaaatg ttaattccat tttaaatgca cagatgtttt
     7261 tatttcataa gggtttcaat gtgcatgaat gctgcaatat tcctgttacc aaagctagta
     7321 taaataaaaa tagataaacg tggaaattac ttagagtttc tgtcattaac gtttccttcc
     7381 tcagttgaca acataaatgc gctgctgaga agccagtttg catctgtcag gatcaatttc
     7441 ccattatgcc agtcatatta attactagtc aattagttga tttttatttt tgacatatac
     7501 atgtgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg
     7561 catggaaaaa tacataactg agaatagaaa agttcagatc aaggtcagga acagatggaa
     7621 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
     7681 caagaacaga tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct
     7741 gccccggctc agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc
     7801 tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat
     7861 ttgaactaac caatcagttc gcttctcgct tctgttcgcg c
//
