LOCUS       sequence-218214-        6639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Entry vector for adenoviral expression of Ecdysone receptor-based 
            inducible transgene expression switch..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6639)
  AUTHORS   Lee S, Sohn KC, Choi DK, Won M, Park KA, Ju SK, Kang K, Bae YK, Hur 
            GM, Ro H
  TITLE     Ecdysone Receptor-based Singular Gene Switches for Regulated 
            Transgene Expression in Cells and Adult Rodent Tissues.
  JOURNAL   Mol Ther Nucleic Acids. 2016 Sep 27;5(9):e367. doi: 
            10.1038/mtna.2016.74.
  PUBMED    27673563
REFERENCE   2  (bases 1 to 6639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2016-09-27"; volume: "5(9):e367. doi"; 
            pages: " 10.1038/mtna.2016.74"
COMMENT     Sequence Label: pENTR-EUI
FEATURES             Location/Qualifiers
     source          1..6639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     enhancer        837..1216
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1217..1420
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1371..1391
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        1465..1483
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1503..1940
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLQSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTV"
     promoter        complement(3045..3063)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3083..3100)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3089..3313
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        4060..4078
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4103..4119
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4104..4120
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4459..4477
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    4591..4725
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4615..4634)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4669..4688
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(4764..4863)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(4881..4899)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4904..4920)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4904..4920)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5022..5041)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             5033..5842
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5100..5119)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5989..6577
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6478..6497
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      721 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
      781 ccgcatagtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      841 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      901 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      961 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     1021 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     1081 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     1141 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     1201 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     1261 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     1321 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     1381 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
     1441 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagcttatcg
     1501 atatgaagct acagtcttct atcgaacaag catgcgatat ttgccgactt aaaaagctca
     1561 agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg gagtgtcgct
     1621 actctcccaa aaccaaaagg tctccgctga ctagggcaca tctgacagaa gtggaatcaa
     1681 ggctagaaag actggaacag ctatttctac tgatttttcc tcgagaagac cttgacatga
     1741 ttttgaaaat ggattcttta caggatataa aagcattgtt aacaggatta tttgtacaag
     1801 ataatgtgaa taaagatgcc gtcacagata gattggcttc agtggagact gatatgcctc
     1861 taacattgag acagcataga ataagtgcga catcatcatc ggaagagagt agtaacaaag
     1921 gtcaaagaca gttgactgta gagttcctgg atatggccga cttcgagttt gagcagatgt
     1981 ttaccgatgc ccttggaatt gacgagtacg gtgggctgca gatgaggcct gaatgtgtca
     2041 tacaggagcc cagtaaaaat aaagacaggc aaagacaaaa gaaagacaaa ggaatattat
     2101 tacctgttag tacgaccaca gtcgaagacc acatgccccc gatcatgcaa tgtgatccac
     2161 ctccgcccga ggccgccagg attcacgaag tcgtcccgag gtatctttcg gagaagctga
     2221 tggagcagaa caggcagaag aacataccac cattgtcggc gaatcagaag tctctgatcg
     2281 cgaggctcgt gtggtaccag gagggatatg agcagccctc cgacgaggat ctcaaaagag
     2341 taacgcagac ttggcagtcg gatgaagagg acgaggaatc cgatctaccc ttccgccaga
     2401 tcacggagat gacgatctta acggtccagt tgatcgtcga gttcgccaag ggtctaccgg
     2461 gcttttcgaa gatatcacag tctgatcaaa tcaccttatt aaaagcctcg tccagcgagg
     2521 tgatgatgct gcgggtggcg aggcgatacg acgccgcgtc cgacagcatt ctgttcgcca
     2581 acaacaaggc gtacacgcgc gacaactacc gcaaggcggg catggccgag gtcatcgaag
     2641 acctcctaca cttctgccgg tgcatgttcg cgatgggcat ggacaatgtg cactttgcac
     2701 tgctcacggc catcgttata ttctcagatc ggcccgggct cgagcagccg tcgctggtag
     2761 aagagatcca gagatactac ctgaacacgt tgcgaattta catcatcaac cagaacagcg
     2821 cgtcgtcgcg ctgcgccgtg atctacggca ggatcctgag cgtgctgacc gagctacgca
     2881 cgctcggcac gcaaaactcc aacatgtgca tctcgctgaa gctgaagaac aggaagctgc
     2941 cgccgttcct cgaggagatc tgggacgtgg cggaggtggc cacgacgcat cccacggtgc
     3001 tgccgcccac caacccggtg gtgctatagt ctagagggcc ctattctata gtgtcaccta
     3061 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     3121 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     3181 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     3241 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     3301 gtgggctcta tggcttctga ggcggaaagt tgagaggaca ttccaatcat aggctgccca
     3361 tccaccctct gtgtcctcct gttaattagg tcacttaaca aaaaggaaat tgggtagggg
     3421 tttttcacag accgctttct aagggtaatt ttaaaatatc tgggaagtcc cttccactgc
     3481 tgtgttccag aagtgttggt aaacagccca caaatgtcaa cagcagaaac atacaagctg
     3541 tcagctttgc acaagggccc aacaccctgc tcatcaagaa gcactgtggt tgctgtgtta
     3601 gtaatgtgca aaacaggagg cacattttcc ccacctgtgt aggttccaaa atatctagtg
     3661 ttttcatttt tacttggatc aggaacccag cactccactg gataagcatt atccttatcc
     3721 aaaacagcct tgtggtcagt gttcatctgc tgactgtcaa ctgtagcatt ttttggggtt
     3781 acagtttgag caggatattt ggtcctgtag tttgctaaca caccctgcag ctccaaaggt
     3841 tccccaccaa cagcaaaaaa atgaaaattt gacccttgaa tgggttttcc agcaccattt
     3901 tcatgagttt tttgtgtccc tgaatgcaag tttaacatag cagttacccc aataacctca
     3961 gttttaacag taacagcttc ccacatcaaa atatttccac aggttaagtc ctcatttaaa
     4021 ttaggcaaag gaattgctca actttgtaag aaaagttggt aatacgactc actatagggc
     4081 gaattgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttag gcctccaagg
     4141 cggagtactg tcctccgggc tggcggagta ctgtcctccg gcaaggtcgg agtactgtcc
     4201 tccgacacta gaggtcggag tactgtcctc cgacgcaagg cggagtactg tcctccgggc
     4261 tgcggagtac tgtcctccgg caaggtcgga gtactgtcct ccgacactag aggtcggagt
     4321 actgtcctcc gacgcaaggc ggagtactgt cctccgggct ggcggagtac tgtcctccgg
     4381 caagggtcga ctctagaggg tatataatgg atcccatcgc gtctcagcct cactttgagc
     4441 tcctccacac ggagcttgat ttaggtgaca ctatagaata caagctactt gttctttttg
     4501 caggatccca tcgattcgaa ttcacgtgct agcgcttaag aaattcaagg cctctcgagc
     4561 ctctagaact atagtgagtc gtattacgta gatccagaca tgataagata cattgatgag
     4621 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     4681 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     4741 attcatttta tgtttcaggg ccgacccagc tttcttgtac aaagttggca ttataagaaa
     4801 gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat
     4861 ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg
     4921 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     4981 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
     5041 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     5101 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
     5161 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     5221 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     5281 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac
     5341 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     5401 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     5461 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     5521 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
     5581 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     5641 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     5701 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     5761 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     5821 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
     5881 tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc
     5941 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6001 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6061 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6121 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6181 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6241 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6301 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6361 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6421 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6481 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6541 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6601 acggttcctg gccttttgct ggccttttgc tcacatgtt
//
