LOCUS       sequence-218247-        8868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the fluorescent protein miRFP670 in a lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8868)
  TITLE     Fluorescent protein expressing plasmids suitable for lentiviral 
            approaches
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti6.2_miRFP670
FEATURES             Location/Qualifiers
     source          1..8868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      18..606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     507..526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     760..777
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    894..915
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(930..935,936..953,954..960)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    968..984
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     973..995
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     992..1008
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     992..1008
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1027..1047
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1029..1047
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1075..1301
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1302..1482
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1529..1654
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2147..2380
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2565..2609
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2876..2992
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3015..3318
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3319..3522
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3473..3493
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3519..3543
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3616..3625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3622..4569
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     misc_feature    4602..5190
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4655..4675)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5073..5084)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5204..5703
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5239..5262)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5636..5655
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5724..6323
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5724..5743)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6220..6240
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6461..6694
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6766..6887
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6803..6822)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6857..6876
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6927..7062
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7082..7101)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7083..7101)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7111..7128)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7111..7127)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7120..7142)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7269..7724
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7356..7375)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7566..7587
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7750..7854
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7855..7923,7924..8715)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8073..8092)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
       61 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
      121 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
      181 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
      241 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
      301 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
      361 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
      421 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      481 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      541 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      601 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      661 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
      721 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      781 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
      841 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
      901 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
      961 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     1021 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     1081 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     1141 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
     1201 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     1261 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     1321 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     1381 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     1441 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     1501 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     1561 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     1621 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     1681 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     1741 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     1801 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1861 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1921 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     1981 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     2041 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     2101 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     2161 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     2221 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     2281 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     2341 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     2401 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     2461 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     2521 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     2581 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     2641 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     2701 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2761 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     2821 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta
     2881 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     2941 cagacataca aactaaagaa ttacaaaaac aaattacaaa attcaaaatt ttatcgataa
     3001 gcttgggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     3061 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     3121 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     3181 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3241 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3301 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     3361 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     3421 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     3481 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
     3541 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg actctagaga
     3601 tatcttcgaa ggcgcgccac catggtagca ggtcatgcct ctggcagccc cgcattcggg
     3661 accgcctctc attcgaattg cgaacatgaa gagatccacc tcgccggctc gatccagccg
     3721 catggcgcgc ttctggtcgt cagcgaacat gatcatcgcg tcatccaggc cagcgccaac
     3781 gccgcggaat ttctgaatct cggaagcgta ctcggcgttc cgctcgccga gatcgacggc
     3841 gatctgttga tcaagatcct gccgcatctc gatcccaccg ccgaaggcat gccggtcgcg
     3901 gtgcgctgcc ggatcggcaa tccctctacg gagtactgcg gtctgatgca tcggcctccg
     3961 gaaggcgggc tgatcatcga actcgaacgt gccggcccgt cgatcgatct gtcaggcacg
     4021 ctggcgccgg cgctggagcg gatccgcacg gcgggttcac tgcgcgcgct gtgcgatgac
     4081 accgtgctgc tgtttcagca gtgcaccggc tacgaccggg tgatggtgta tcgtttcgat
     4141 gagcaaggcc acggcctggt attctccgag tgccatgtgc ctgggctcga atcctatttc
     4201 ggcaaccgct atccgtcgtc gactgtcccg cagatggcgc ggcagctgta cgtgcggcag
     4261 cgcgtccgcg tgctggtcga cgtcacctat cagccggtgc cgctggagcc gcggctgtcg
     4321 ccgctgaccg ggcgcgatct cgacatgtcg ggctgcttcc tgcgctcgat gtcgccgtgc
     4381 catctgcagt tcctgaagga catgggcgtg cgcgccaccc tggcggtgtc gctggtggtc
     4441 ggcggcaagc tgtggggcct ggttgtctgt caccattatc tgccgcgctt catccgtttc
     4501 gagctgcggg cgatctgcaa acggctcgcc gaaaggatcg cgacgcggat caccgcgctt
     4561 gagagctaag cggccgcacg cgtgcaatca acctcgtcga caatcaacct ctggattaca
     4621 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4681 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4741 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4801 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4861 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     4921 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     4981 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     5041 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5101 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5161 gtcggatctc cctttgggcc gcctccccgc ctggaattct accgggtagg ggaggcgctt
     5221 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     5281 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     5341 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     5401 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     5461 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     5521 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
     5581 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
     5641 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     5701 tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     5761 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     5821 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     5881 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     5941 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     6001 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     6061 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     6121 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     6181 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     6241 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     6301 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
     6361 gcgcacgacc ccatgcatct cgagggcccg gtacctttaa gaccaatgac ttacaaggca
     6421 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct agctcactcc
     6481 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     6541 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6601 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6661 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     6721 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     6781 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6841 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     6901 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6961 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     7021 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     7081 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     7141 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     7201 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     7261 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     7321 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     7381 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     7441 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     7501 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     7561 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     7621 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     7681 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     7741 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7801 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7861 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7921 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7981 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8041 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     8101 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8161 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8221 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8281 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8341 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8401 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8461 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8521 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8581 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8641 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8701 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8761 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8821 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaa
//
