LOCUS       sequence-218248-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes pCUP1 as a Type 2 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK031
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        117..351
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
     terminator      complement(430..464)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
       61 tatcccctga ttctgtggat aaccgtagtc ggtctcaaac gatcttagcc ttgttgctag
      121 ttagaaaaag acatttttgc tgtcagtcac tgtcaagaga ttcttttgct ggcatttctt
      181 ctaggagcaa aaagagcgat gcgtcttttc cgctgaaccg ttccagcaaa aaagactacc
      241 aacgcaatat ggattgtcag aatcatataa aagagaagca aataactcct tgtcttgtat
      301 caattgcatt ataatatctt cttgttagtg caatatcata tagaagtcat cgaaatagat
      361 attaagaaaa acaaactgta caatcaatca atcaatcatc acataaaaga tctatgtgag
      421 accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
      481 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
      541 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
      601 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
      661 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
      721 taaatcaaaa ctggtgaaac tcacccaggg attggctgaa c
//
