LOCUS       sequence-21825-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  TITLE     Second Batch
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-HA-GSTM5
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     63..82
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        63..81
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             107..133
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(836..854)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(837..854)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(874..891)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 cgggggcntt agcagagctc tctggctact agagaaccca ctgcttactg gcttatcgaa
       61 attaatacga ctcactatag ggagacccaa gcttgccgcc accatgtacc cttatgatgt
      121 gccagattat gcctctgaat tcgccgccac catgcccatg actctggggt actgggacat
      181 ccgtgggctg gcccacgcca tccgcttgct cctggaatac acagactcaa gctatgtgga
      241 aaagaagtac acgctggggg acgctcctga ctatgacaga agccagtggc tgaatgaaaa
      301 attcaagctg ggcctggact ttcccaatct gccctacttg attgatgggg ctcacaagat
      361 cacccagagc aatgccatcc tgcgctacat tgcccgcaag cacaacctgt gtggggagac
      421 agaagaggag aagattcgtg tggacatttt ggagaaccag gttatggata accacatgga
      481 gctggtcaga ctgtgctatg acccagattt tgagaaactg aagccaaaat acttggagga
      541 actccctgaa aagctaaagc tctactcaga gtttctgggg aagcggcctt ggtttgcagg
      601 agacaagatc acctttgtgg atttccttgc ctatgatgtc cttgacatga agcgtatatt
      661 tgagcccaag tgcttggacg ccttcctaaa cttgaaggac ttcatctccc gctttgaggg
      721 tttgaagaag atctctgcct acatgaagtc cagccaattc ctccgaggtc ttttgtttgg
      781 aaagtcagct acatggaaca gcaaatagct cgagcatgca tctagagggc cctattctat
      841 agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
      901 catctgttgt ttgcccctcc ccgtgctctg actgagggca ncccgctcca tgtctttcta
      961 tantaa
//
