LOCUS       sequence-218253-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Cas9 as a Type 3 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK036
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
       61 ctttcctgcg ttatcccctg attctgtgga taaccgtagt cggtctccta tggacaagaa
      121 gtattctatc ggactggata tcgggactaa tagcgtcggg tgggccgtga tcactgacga
      181 gtacaaggtg ccctctaaga agttcaaggt gctcgggaac accgaccggc attccatcaa
      241 gaaaaatctg atcggagctc tcctctttga ttcaggggaa accgctgaag caacccgcct
      301 caagcggact gctagacggc ggtacaccag gaggaagaac cggatttgtt accttcaaga
      361 gatattctcc aacgaaatgg caaaggtcga cgacagcttc ttccataggc tggaagaatc
      421 attcctcgtg gaagaggata agaagcatga acggcatccc atcttcggta atatcgtcga
      481 cgaggtggcc tatcacgaga aatacccaac catctaccat cttcgcaaaa agctggtgga
      541 ctcaaccgac aaggcagacc tccggcttat ctacctggcc ctggcccaca tgatcaagtt
      601 cagaggccac ttcctgatcg agggcgacct caatcctgac aatagcgatg tggataaact
      661 gttcatccag ctggtgcaga cttacaacca gctctttgaa gagaacccca tcaatgcaag
      721 cggagtcgat gccaaggcca ttctgtcagc ccggctgtca aagagccgca gacttgagaa
      781 tcttatcgct cagctgccgg gtgaaaagaa aaatggactg ttcgggaacc tgattgctct
      841 ttcacttggg ctgactccca atttcaagtc taatttcgac ctggcagagg atgccaagct
      901 gc
//
