LOCUS       sequence-218273-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes tPGK1 as a Type 4 part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK054
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(363..397)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
       61 tttcctgcgt tatcccctga ttctgtggat aaccgtagtc ggtctcaatc ctaactcgag
      121 attgaattga attgaaatcg atagatcaat ttttttcttt tctctttccc catcctttac
      181 gctaaaataa tagtttattt tattttttga atatttttta tttatatacg tatatataga
      241 ctattattta tcttttaatg attattaaga tttttattaa aaaaaaattc gctcctcttt
      301 taatgccttt atgcagtttt tttttcccat tcgatatttc tatgtgctgt gagaccagac
      361 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
      421 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
      481 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
      541 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
      601 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
      661 aaactggtga aactcaccca gggattggct gaaacgaaaa acatattctc aataaaccct
      721 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
      781 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
      841 tggaaaac
//
