LOCUS       sequence-218275-        1146 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral system for reduced ectopic expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Dean KM, Roudot P, Reis CR, Welf ES, Mettlen M, Fiolka R
  TITLE     Diagonally Scanned Light-Sheet Microscopy for Fast Volumetric 
            Imaging of Adherent Cells.
  JOURNAL   Biophys J. 2016 Mar 29;110(6):1456-65. doi: 
            10.1016/j.bpj.2016.01.029.
  PUBMED    27028654
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2016.01"; journalName: "Biophys J"; date: 
            "2016-03-29- 29"; volume: "110"; issue: "6"; pages: "1456-65"
COMMENT     Sequence Label: pLVX-CMV600
FEATURES             Location/Qualifiers
     source          1..1146
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    117..173
                     /label=MCS
                     /note="multiple cloning site"
     promoter        complement(212..415)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(241..261)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        416..719
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    820..935
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 tgcattcntt tnnnntttca gggttcagng gggaaggtng tgggaaggtt ttttaaagca
       61 agtaaaacct ctacaaatgt ggtatggctg attatgatca gttatctaga tccggtggat
      121 cccgggcccg cggtaccgtc gactgcagaa ttcgaagctt gagctcgaga tctactagtt
      181 agcgctagcg gatctgacgg ttcactaaac cagctctgct tatatagacc tcccaccgta
      241 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
      301 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
      361 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg
      421 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
      481 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
      541 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
      601 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
      661 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
      721 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactat
      781 cgataaactg gatctctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt
      841 tgtttttgta attctttant ttgtatgtct gttgctatta tgtctactat tctttcccct
      901 gcactgtacc ccccaatccc cccttttctt ttaaaggcga taccgtcgag atccgttcac
      961 taatcgaatg gatctgtctc tgtctctctc tccaccttct tcttctattc cttcgggcct
     1021 gtcgnncccc tngggttggg gangtnnggn cnngaaannn nannnnnaat antcccntnn
     1081 nnnnntncnn nttnncnnat annnangtan nngnnaaaan nantnnttnn nancnancan
     1141 annnnc
//
