LOCUS       sequence-218276-        6297 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Actin Related Protein, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6297)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-ARPp34-C-14
FEATURES             Location/Qualifiers
     source          1..6297
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             553..1230
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1279..1953
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     polyA_signal    3025..3146
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3062..3081)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3116..3135
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3153..3608)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3290..3311)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3502..3521
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3635..3739
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3741..4098
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3741..3932
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3764..3784)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3949..4084
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4133..4927
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4133..4927
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4187..4206)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4797..4816
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5115..5134)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5159..5206
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5535..6123
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6024..6043
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatgagtgc gattaagcca gacatgaaga tcaacctccg tatggaaggc
      601 aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga
      661 aaacagagta tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc
      721 ctgaccactg cattccatta cggcaacagg gtattcgccg aatatccaga ccacatacaa
      781 gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa
      841 gacgggggca tttgcattgc cagaaacgac ataacaatgg aaggggacac tttctataat
      901 aaagttcgat ttcacggtgt aaactttccc gccaatggtc cagttatgca gaagaagacg
      961 ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct gactggtgat
     1021 attcgcatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact
     1081 tacaaagcta aggagaaggg tgtcaagtta ccaggctacc actttgtgga ccactgcatt
     1141 gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct
     1201 cattctggat tgcctgacaa tgccagacga tccggtagcg gtcacggtac tggttctact
     1261 ggttctggtt cttctgagag tgcgattaag ccagacatga agatcaacct ccgtatggaa
     1321 ggcaacgtaa acgggcacca ctttgtgatc gacggagatg gtacaggcaa gccttttgag
     1381 ggaaaacaga gtatggatct tgaagtcaaa gagggcggac ctctgccttt tgcctttgat
     1441 atcctgacca ctgcattcca ttacggcaac agggtattcg ccgaatatcc agaccacata
     1501 caagactatt ttaagcagtc gtttcctaag gggtattcgt gggaacgaag cttgactttc
     1561 gaagacgggg gcatttgcat tgccagaaac gacataacaa tggaagggga cactttctat
     1621 aataaagttc gatttcacgg tgtaaacttt cccgccaatg gtccagttat gcagaagaag
     1681 acgctgaaat gggagccctc cactgagaaa atgtatgtgc gtgatggagt gctgactggt
     1741 gatattcgca tggctttgtt gcttgaagga aatgcccatt accgatgtga cttcagaact
     1801 acttacaaag ctaaggagaa gggtgtcaag ttaccaggct accactttgt ggaccactgc
     1861 attgagattt taagccatga caaagattac aacaaggtta agctgtatga gcatgctgtt
     1921 gctcattctg gattgcctga caatgccaga cgatccggaa gtgctgggag cgcaagcgga
     1981 agcggaggct ccggtatcct gctggaggtg aacaaccgca tcatcgagga gacgctcgcg
     2041 ctcaagttcg agaacgcagc cgccggaaac aaaccggaag cagtagaagt aacatttgca
     2101 gatttcgatg gggtcctcta tcatatttca aatcctaatg gagacaaaac aaaagtgatg
     2161 gtcagtattt ctttgaaatt ctacaaggaa cttcaggcac atggtgctga tgagttatta
     2221 aagagggtgt acgggagttt cttggtaaat ccagaatcag gatacaatgt ctctttgcta
     2281 tatgaccttg aaaatcttcc ggcatccaag gattccattg tgcatcaagc tggcatgttg
     2341 aagcgaaatt gttttgcctc tgtctttgaa aaatacttcc aattccaaga agagggcaag
     2401 gaaggagaga acagggcagt tatccattat agggatgatg agaccatgta tgttgagtct
     2461 aaaaaggaca gagtcacagt agtcttcagc acagtgttta aggatgacga cgatgtggtc
     2521 attggaaagg tgttcatgca ggagttcaaa gaaggacgca gagccagcca cacagcccca
     2581 caggtcctct ttagccacag ggaacctcct ctggagctga aagacacaga cgccgctgtg
     2641 ggtgacaaca ttggctacat tacctttgtg ctgttccctc gtcacaccaa tgccagtgca
     2701 cgagacaaca ccatcaacct gatccacacg ttccgggact acctgcacta ccacatcaag
     2761 tgctctaagg cctatattca cacacgtatg cgggcgaaaa cgtctgactt cctcaaggtg
     2821 ctgaaccgcg cacgcccaga tgccgagaaa aaagaaatga aaacaatcac ggggaagacg
     2881 ttttcatccc gctaaggatc caccggatct agataactga tcataatcag ccataccaca
     2941 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     3001 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     3061 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3121 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa
     3181 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     3241 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     3301 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     3361 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     3421 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     3481 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     3541 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     3601 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     3661 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     3721 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     3781 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     3841 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3901 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     3961 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4021 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4081 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     4141 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     4201 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     4261 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     4321 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     4381 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4441 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     4501 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     4561 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     4621 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     4681 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     4741 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     4801 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     4861 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     4921 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     4981 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     5041 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     5101 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     5161 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     5221 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     5281 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     5341 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
     5401 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5461 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5521 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5581 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     5641 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     5701 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     5761 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     5821 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     5881 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     5941 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6001 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6061 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6121 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6181 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcattag
     6241 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccg
//
