LOCUS       sequence-218277-        1116 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral system for reduced ectopic expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Dean KM, Roudot P, Reis CR, Welf ES, Mettlen M, Fiolka R
  TITLE     Diagonally Scanned Light-Sheet Microscopy for Fast Volumetric 
            Imaging of Adherent Cells.
  JOURNAL   Biophys J. 2016 Mar 29;110(6):1456-65. doi: 
            10.1016/j.bpj.2016.01.029.
  PUBMED    27028654
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2016.01"; journalName: "Biophys J"; date: 
            "2016-03-29- 29"; volume: "110"; issue: "6"; pages: "1456-65"
COMMENT     Sequence Label: pLVX-CMV500
FEATURES             Location/Qualifiers
     source          1..1116
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    112..168
                     /label=MCS
                     /note="multiple cloning site"
     enhancer        323..626
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    727..842
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 tgcattcntt ttatgtttcn ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
       61 aacctctaca aatgtggtat ggctgattat gatcagttat ctagatccgg tggatcccgg
      121 gcccgcggta ccgtcgactg cagaattcga agcttgagct cgagatctac tagttagcgc
      181 tagcggtacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
      241 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
      301 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
      361 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
      421 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
      481 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
      541 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
      601 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
      661 ccgtaattga ttactattaa taactatcga taaactggat ctctgctgtc cctgtaataa
      721 acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt
      781 gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta
      841 aaggcgatac cgtcgagatc cgttcactaa tcgaatggat ctgtctctgt ctctctctcc
      901 accttcttct tctattcctt cggnctgtcg ggtcccctcg gggttgggga ggtgggtcct
      961 gaacnataat gnngaatatc ccngccnaac tctattnnct anannaaagt acagcaaaac
     1021 tattnntaan ntaccagcct cctactatcn tnntgnnnnt ttnnnnnncn cancnanttn
     1081 nnatnnnnan nnantncnnn naacttnncn cnnttt
//
