LOCUS       sequence-218278-        1145 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral system for reduced ectopic expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Dean KM, Roudot P, Reis CR, Welf ES, Mettlen M, Fiolka R
  TITLE     Diagonally Scanned Light-Sheet Microscopy for Fast Volumetric 
            Imaging of Adherent Cells.
  JOURNAL   Biophys J. 2016 Mar 29;110(6):1456-65. doi: 
            10.1016/j.bpj.2016.01.029.
  PUBMED    27028654
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2016.01"; journalName: "Biophys J"; date: 
            "2016-03-29- 29"; volume: "110"; issue: "6"; pages: "1456-65"
COMMENT     Sequence Label: pLVX-CMV400
FEATURES             Location/Qualifiers
     source          1..1145
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    112..168
                     /label=MCS
                     /note="multiple cloning site"
     enhancer        223..526
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    627..742
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     gap             1068..1077
                     /estimated_length=10
ORIGIN
        1 tgcattcntt ttatgtttcn ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
       61 aacctctaca aatgtggtat ggctgattat gatcagttat ctagatccgg tggatcccgg
      121 gcccgcggta ccgtcgactg cagaattcga agcttgagct cgagatctac tagttagcgc
      181 tagcggtcca cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac
      241 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
      301 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
      361 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
      421 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
      481 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
      541 tgggctatga actaatgacc ccgtaattga ttactattaa taactatcga taaactggat
      601 ctctgctgtc cctgtaataa acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt
      661 ctttagtttg tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc
      721 caatcccccc ttttctttta aaggcgatac cgtcgagatc cgttcactaa tcgaatggat
      781 ctgtctctgt ctctctctcc accttcttct tctattcctt cgggcctgtc gggtcccctc
      841 ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac tctattcact
      901 atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat cattatgaat
      961 aattttatat accacancca attgtnatgt taaaccaatt tccacaaact tgccatttat
     1021 ctaatncaan nnntnnnatt cttttcntgc tgtttgcgat nntcattnnn nnnnnnntag
     1081 cttgngnnaa nntatttcnc tnntcccnnn ncatncaggt cnnnnngant nncaannnnn
     1141 ntnnc
//
