LOCUS       sequence-218280-        1153 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral system for reduced ectopic expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Dean KM, Roudot P, Reis CR, Welf ES, Mettlen M, Fiolka R
  TITLE     Diagonally Scanned Light-Sheet Microscopy for Fast Volumetric 
            Imaging of Adherent Cells.
  JOURNAL   Biophys J. 2016 Mar 29;110(6):1456-65. doi: 
            10.1016/j.bpj.2016.01.029.
  PUBMED    27028654
REFERENCE   2  (bases 1 to 1153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2016.01"; journalName: "Biophys J"; date: 
            "2016-03-29- 29"; volume: "110"; issue: "6"; pages: "1456-65"
COMMENT     Sequence Label: pLVX-CMV200
FEATURES             Location/Qualifiers
     source          1..1153
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    114..170
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    429..544
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(807..851)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     gap             1064..1077
                     /estimated_length=14
ORIGIN
        1 tgcattcntt tnntgtttca ggttcngggg gnaggtgtgg gnnggttttt taaagcaagt
       61 aaaacctcta caaatgtggt atggctgatt atgatcagtt atctagatcc ggtggatccc
      121 gggcccgcgg taccgtcgac tgcagaattc gaagcttgag ctcgagatct actagttagc
      181 gctagcggac cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac
      241 tatgggaacg tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg
      301 cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga
      361 ccccgtaatt gattactatt aataactatc gataaactgg atctctgctg tccctgtaat
      421 aaacccgaaa attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg
      481 ttgctattat gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt
      541 taaaggcgat accgtcgaga tccgttcact aatcgaatgg atctgtctct gtctctctct
      601 ccaccttctt cttctattcc ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc
      661 tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa
      721 actattctta aacctaccaa gcctcctact atcattatga ataattttat ataccacagc
      781 caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct
      841 tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat taagcttgtg
      901 taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat ctgttccaga
      961 gatttattac tccaactagc attccangca cagcagtggn gcaaatgagt tttccagagc
     1021 aaccccaatc cccagnagct nnnatctttn nnatctttcc ncannnnnnn nnnnnnnctg
     1081 ntnatgccca nactgnnnnn ncaacnnnan gcnnnngcnc cctcaannnc nncnnnnaaa
     1141 ttnnntnnnn ctg
//
