LOCUS       sequence-218282-        6793 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Shtengel G, Galbraith JA, Galbraith CG, Lippincott-Schwartz J, 
            Gillette JM, Manley S, Sougrat R, Waterman CM, Kanchanawong P, 
            Davidson MW, Fetter RD, Hess HF
  TITLE     Interferometric fluorescent super-resolution microscopy resolves 3D 
            cellular ultrastructure.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3125-30. doi: 
            10.1073/pnas.0813131106. Epub 2009 Feb 6.
  PUBMED    19202073
REFERENCE   2  (bases 1 to 6793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0813131106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-3- 3"; volume: "106"; issue: "9"; pages: "3125-30"
COMMENT     Sequence Label: tdEos-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..6793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1996..2673
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             2722..3399
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     polyA_signal    3521..3642
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3558..3577)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3612..3631
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3649..4104)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3786..3807)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3998..4017
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4131..4235
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4237..4594
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4237..4428
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4260..4280)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4445..4580
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4629..5423
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4629..5423
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4683..4702)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5293..5312
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5611..5630)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5655..5702
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6031..6619
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6520..6539
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagct caagcttcga attcccatgt ccaccaggtc cgtgtcctcg
      601 tcctcctacc gcaggatgtt cggcggcccg ggcaccgcga gccggccgag ctccagccgg
      661 agctacgtga ctacgtccac ccgcacctac agcctgggca gcgcgctgcg ccccagcacc
      721 agccgcagcc tctacgcctc gtccccgggc ggcgtgtatg ccacgcgctc ctctgccgtg
      781 cgcctgcgga gcagcgtgcc cggggtgcgg ctcctgcagg actcggtgga cttctcgctg
      841 gccgacgcca tcaacaccga gttcaagaac acccgcacca acgagaaggt ggagctgcag
      901 gagctgaatg accgcttcgc caactacatc gacaaggtgc gcttcctgga gcagcagaat
      961 aagatcctgc tggccgagct cgagcagctc aagggccaag gcaagtcgcg cctgggggac
     1021 ctctacgagg aggagatgcg ggagctgcgc cggcaggtgg accagctaac caacgacaaa
     1081 gcccgcgtcg aggtggagcg cgacaacctg gccgaggaca tcatgcgcct ccgggagaaa
     1141 ttgcaggagg agatgcttca gagagaggaa gccgaaaaca ccctgcaatc tttcagacag
     1201 gatgttgaca atgcgtctct ggcacgtctt gaccttgaac gcaaagtgga atctttgcaa
     1261 gaagagattg cctttttgaa gaaactccac gaagaggaaa tccaggagct gcaggctcag
     1321 attcaggaac agcatgtcca aatcgatgtg gatgtttcca agcctgacct cacggctgcc
     1381 ctgcgtgacg tacgtcagca atatgaaagt gtggctgcca agaacctgca ggaggcagaa
     1441 gaatggtaca aatccaagtt tgctgacctc tctgaggctg ccaaccggaa caatgacgcc
     1501 ctgcgccagg caaagcagga gtccactgag taccggagac aggtgcagtc cctcacctgt
     1561 gaagtggatg cccttaaagg aaccaatgag tccctggaac gccagatgcg tgaaatggaa
     1621 gagaactttg ccgttgaagc tgctaactac caagacacta ttggccgcct gcaggatgag
     1681 attcagaata tgaaggagga aatggctcgt caccttcgtg aataccaaga cctgctcaat
     1741 gttaagatgg cccttgacat tgagattgcc acctacagga agctgctgga aggcgaggag
     1801 agcaggattt ctctgcctct tccaaacttt tcctccctga acctgaggga aactaatctg
     1861 gattcactcc ctctggttga tacccactca aaaaggacac ttctgattaa gacggttgaa
     1921 actagagatg gacaggttat caacgaaact tctcagcatc acgatgacct tgaaggggat
     1981 ccaccggtcg ccaccatgag tgcgattaag ccagacatga agatcaacct ccgtatggaa
     2041 ggcaacgtaa acgggcacca ctttgtgatc gacggagatg gtacaggcaa gccttttgag
     2101 ggaaaacaga gtatggatct tgaagtcaaa gagggcggac ctctgccttt tgcctttgat
     2161 atcctgacca ctgcattcca ttacggcaac agggtattcg ccgaatatcc agaccacata
     2221 caagactatt ttaagcagtc gtttcctaag gggtattcgt gggaacgaag cttgactttc
     2281 gaagacgggg gcatttgcat tgccagaaac gacataacaa tggaagggga cactttctat
     2341 aataaagttc gatttcacgg tgtaaacttt cccgccaatg gtccagttat gcagaagaag
     2401 acgctgaaat gggagccctc cactgagaaa atgtatgtgc gtgatggagt gctgactggt
     2461 gatattcgca tggctttgtt gcttgaagga aatgcccatt accgatgtga cttcagaact
     2521 acttacaaag ctaaggagaa gggtgtcaag ttaccaggct accactttgt ggaccactgc
     2581 attgagattt taagccatga caaagattac aacaaggtta agctgtatga gcatgctgtt
     2641 gctcattctg gattgcctga caatgccaga cgatccggta gcggtcacgg tactggttct
     2701 actggttctg gttcttctga gagtgcgatt aagccagaca tgaagatcaa cctccgtatg
     2761 gaaggcaacg taaacgggca ccactttgtg atcgacggag atggtacagg caagcctttt
     2821 gagggaaaac agagtatgga tcttgaagtc aaagagggcg gacctctgcc ttttgccttt
     2881 gatatcctga ccactgcatt ccattacggc aacagggtat tcgccgaata tccagaccac
     2941 atacaagact attttaagca gtcgtttcct aaggggtatt cgtgggaacg aagcttgact
     3001 ttcgaagacg ggggcatttg cattgccaga aacgacataa caatggaagg ggacactttc
     3061 tataataaag ttcgatttca cggtgtaaac tttcccgcca atggtccagt tatgcagaag
     3121 aagacgctga aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg agtgctgact
     3181 ggtgatattc gcatggcttt gttgcttgaa ggaaatgccc attaccgatg tgacttcaga
     3241 actacttaca aagctaagga gaagggtgtc aagttaccag gctaccactt tgtggaccac
     3301 tgcattgaga ttttaagcca tgacaaagat tacaacaagg ttaagctgta tgagcatgct
     3361 gttgctcatt ctggattgcc tgacaatgcc agacgataag cggccgcgac tctagatcat
     3421 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     3481 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     3541 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3601 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg
     3661 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     3721 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     3781 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     3841 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     3901 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     3961 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     4021 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     4081 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4141 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4201 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     4261 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4321 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     4381 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4441 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4501 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     4561 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     4621 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     4681 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     4741 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     4801 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     4861 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     4921 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     4981 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     5041 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     5101 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     5161 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     5221 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     5281 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     5341 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     5401 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     5461 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     5521 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     5581 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     5641 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     5701 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     5761 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     5821 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     5881 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5941 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6001 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6061 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6121 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     6181 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6241 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6301 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6361 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6421 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6481 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6541 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6601 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6661 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6721 taccgccatg cattagttat taatagtaat caattacggg gtcattagtt catagcccat
     6781 atatggagtt ccg
//
