LOCUS       sequence-218286-        7062 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Kanchanawong P, Shtengel G, Pasapera AM, Ramko EB, Davidson MW, Hess
            HF, Waterman CM
  TITLE     Nanoscale architecture of integrin-based cell adhesions.
  JOURNAL   Nature. 2010 Nov 25;468(7323):580-4. doi: 10.1038/nature09621.
  PUBMED    21107430
REFERENCE   2  (bases 1 to 7062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-11-25- 25"; volume: "468"; issue: "7323"; 
            pages: "580-4"
COMMENT     Sequence Label: tdEos-Paxillin-KM-C-10
FEATURES             Location/Qualifiers
     source          1..7062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             553..1230
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1279..1953
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     polyA_signal    3790..3911
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3827..3846)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3881..3900
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3918..4373)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4055..4076)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4267..4286
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4400..4504
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4506..4863
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4506..4697
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4529..4549)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4714..4849
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4898..5692
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4898..5692
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4952..4971)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5562..5581
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5880..5899)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5924..5971
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6300..6888
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6789..6808
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatgagtgc gattaagcca gacatgaaga tcaacctccg tatggaaggc
      601 aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga
      661 aaacagagta tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc
      721 ctgaccactg cattccatta cggcaacagg gtattcgccg aatatccaga ccacatacaa
      781 gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa
      841 gacgggggca tttgcattgc cagaaacgac ataacaatgg aaggggacac tttctataat
      901 aaagttcgat ttcacggtgt aaactttccc gccaatggtc cagttatgca gaagaagacg
      961 ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct gactggtgat
     1021 attcgcatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact
     1081 tacaaagcta aggagaaggg tgtcaagtta ccaggctacc actttgtgga ccactgcatt
     1141 gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct
     1201 cattctggat tgcctgacaa tgccagacga tccggtagcg gtcacggtac tggttctact
     1261 ggttctggtt cttctgagag tgcgattaag ccagacatga agatcaacct ccgtatggaa
     1321 ggcaacgtaa acgggcacca ctttgtgatc gacggagatg gtacaggcaa gccttttgag
     1381 ggaaaacaga gtatggatct tgaagtcaaa gagggcggac ctctgccttt tgcctttgat
     1441 atcctgacca ctgcattcca ttacggcaac agggtattcg ccgaatatcc agaccacata
     1501 caagactatt ttaagcagtc gtttcctaag gggtattcgt gggaacgaag cttgactttc
     1561 gaagacgggg gcatttgcat tgccagaaac gacataacaa tggaagggga cactttctat
     1621 aataaagttc gatttcacgg tgtaaacttt cccgccaatg gtccagttat gcagaagaag
     1681 acgctgaaat gggagccctc cactgagaaa atgtatgtgc gtgatggagt gctgactggt
     1741 gatattcgca tggctttgtt gcttgaagga aatgcccatt accgatgtga cttcagaact
     1801 acttacaaag ctaaggagaa gggtgtcaag ttaccaggct accactttgt ggaccactgc
     1861 attgagattt taagccatga caaagattac aacaaggtta agctgtatga gcatgctgtt
     1921 gctcattctg gattgcctga caatgccaga cgatccggac tcagatctgg cagcagtgga
     1981 ggtgacgacc tcgatgcctt actggcagac ctggaatcca ccacctcgca tatctctaag
     2041 cgaccggtgt ttctaacaga ggaaacgcct tactcctacc caactggaaa ccatacgtac
     2101 caggagattg ctgtgccacc gccggtgccc ccgccgcctt ccagtgaggc cctaaatggc
     2161 actgtgattg accccttaga ccagtggcaa cccagcgtgt ccagatacgg ccaccagcaa
     2221 cctcagtccc agtctccgat atacagctct agtgccaaaa gctccagtgc ctctgttcct
     2281 agagacgggc tcagctcccc ttctccccgt gccagtgagg aggaacacgt gtacagcttc
     2341 ccaaacaagc agaagtctgc agaaccatct ccctcactga ccagcacctc cctgggcagc
     2401 aacctctcgg aactggacag gcttctcctg gaactgaatg ctgttcaaca taatcccccc
     2461 agtggcttct cagcagatga agtcagcaga agcccatcac tgcccaatgt gaccggacct
     2521 cactatgtca tcccagagag cagcagctct gcaggaggga aggctgcacc ccctacaaaa
     2581 gaaaaaccaa agcggaatgg tgggcgtggg attgaagatg tgcgtcccag tgtggagagc
     2641 ctgctggatg agctggagag ctctgtgcca agcccagtcc ctgcaatcac tgtgagccaa
     2701 ggggaggtga gcagccccca gcgtgtcaac gccagtcagc agcagacccg tatctctgct
     2761 tcttcagcta cacgagaact ggatgagctg atggcgtccc tctctgactt taagttcatg
     2821 gcacagggga aagccggcgg cagcagctcc ccaccatcca ccaccccaaa gcctggcagt
     2881 cagctggaca ccatgctggg aagtctgcag tctgacctga acaaactggg ggtagcgacg
     2941 gtcgccaagg gtgtgtgcgg agcctgcaag aagccgattg ctgggcaggt agttacagcc
     3001 atggggaaga cctggcaccc tgagcacttc gtctgcaccc actgccagga ggagattgga
     3061 tcacggaact tctttgagcg ggatgggcag ccctactgtg agaaggacta tcacaacctc
     3121 ttctctcctc gctgctacta ctgcaatggg ccaatccttg ataaagtggt gacggctttg
     3181 gacaggacgt ggcaccctga acactttttc tgtgcccagt gtggagtttt ctttggacct
     3241 gaaggatttc atgagaagga tggcaaagcc tattgccgca aggactactt tgacatgttt
     3301 gctcccaagt gtggaggctg tgcccgggct atcctggaaa attacatttc tgccttaaac
     3361 accctgtggc accctgagtg ctttgtctgt cgggaatgtt tcacaccgtt catcaacggc
     3421 agcttcttcg agcacgacgg gcagccctac tgcgaggtgc attaccacga gcgccgcggc
     3481 tcgctgtgct ctggctgcca gaagcccatc acgggacgct gcatcactgc catgggcaag
     3541 aaatttcacc ccgaacactt tgtctgtgcc ttctgcctca agcagctcaa caaaggaacc
     3601 ttcaaagaac agaacgacaa gccctactgt cagaactgct ttctcaaact cttctgttaa
     3661 ggatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
     3721 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     3781 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3841 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3901 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     3961 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     4021 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     4081 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     4141 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     4201 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     4261 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     4321 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     4381 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4441 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4501 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4561 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4621 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4681 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4741 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4801 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4861 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     4921 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     4981 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     5041 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     5101 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     5161 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     5221 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     5281 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     5341 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     5401 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     5461 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     5521 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     5581 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     5641 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     5701 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     5761 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     5821 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     5881 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     5941 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     6001 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     6061 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     6121 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     6181 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     6241 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     6301 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     6361 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     6421 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6481 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6541 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6601 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6661 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6721 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6781 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6841 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6901 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6961 ttatcccctg attctgtgga taaccgtatt accgccatgc attagttatt aatagtaatc
     7021 aattacgggg tcattagttc atagcccata tatggagttc cg
//
