LOCUS       sequence-218295-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes 6XHis_3XFlag as a Type 4a part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK060
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             112..183
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             187..204
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(231..265)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
       61 tttcctgcgt tatcccctga ttctgtggat aaccgtagtc ggtctcaatc cgattacaag
      121 gacgatgatg ataaggacta caaagatgat gatgacaaag attataagga cgatgatgat
      181 aaaggtcatc atcatcatca tcactaactc gagtggctga gaccagacca ataaaaaacg
      241 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
      301 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg
      361 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
      421 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
      481 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
      541 ctcacccagg gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag
      601 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
      661 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
      721 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
      781 tccggatgag cattcatcag gcgggcaaga atgtgaat
//
