LOCUS       sequence-218297-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes tPGK1 as a Type 4b part to be used in the Dueber YTK system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK064
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(354..388)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
ORIGIN
        1 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
       61 tttcctgcgt tatcccctga ttctgtggat aaccgtagtc ggtctcctgg cattgaattg
      121 aattgaaatc gatagatcaa tttttttctt ttctctttcc ccatccttta cgctaaaata
      181 atagtttatt ttattttttg aatatttttt atttatatac gtatatatag actattattt
      241 atcttttaat gattattaag atttttatta aaaaaaaatt cgctcctctt ttaatgcctt
      301 tatgcagttt ttttttccca ttcgatattt ctatgtgctg tgagaccaga ccaataaaaa
      361 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
      421 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca
      481 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
      541 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
      601 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
      661 aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc tttagggaaa
      721 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
      781 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
      841 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
      901 aattccggat gagcattcat cag
//
