LOCUS       sequence-218314-         648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes KanamycinR as a Type 6 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK077
FEATURES             Location/Qualifiers
     source          1..648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(562..581)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
       61 tttcctgcgt tatcccctga ttctgtggat aaccgtagtc ggtctcctac agtttagctt
      121 gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac
      181 agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata
      241 catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa
      301 aattacggct cctcgctcca gacctgcgag cagggaaacg ctcccctcac agacgcgttg
      361 aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg
      421 ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc
      481 gaacataaac aaaaatgggt aaggaaaaga ctcacgtttc gaggccgcga ttaaattcca
      541 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
      601 cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttc
//
