LOCUS       sequence-218344-        9830 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral NanoLuc control expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Trepte P, Kruse S, Kostova S, Hoffmann S, Buntru A, Tempelmeier A, 
            Secker C, Diez L, Schulz A, Klockmeier K, Zenkner M, Golusik S, Rau 
            K, Schnoegl S, Garner CC, Wanker EE
  TITLE     LuTHy: a double-readout bioluminescence-based two-hybrid technology 
            for quantitative mapping of protein-protein interactions in 
            mammalian cells.
  JOURNAL   Mol Syst Biol. 2018 Jul 11;14(7):e8071. doi: 10.15252/msb.20178071.
  PUBMED    29997244
REFERENCE   2  (bases 1 to 9830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2018-07-11"; volume: "14(7):e8071. doi"; pages: " 
            10.15252/msb.20178071"
COMMENT     Sequence Label: pLenti NL
FEATURES             Location/Qualifiers
     source          1..9830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        55..1266
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1103..1122
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             1304..1333
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1334..1846
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     misc_feature    1955..2543
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2008..2028)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2426..2437)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2546..2562)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2547..2563)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3068..3248
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3274..3291)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3280..3504
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3550..3978
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3637..3656)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3847..3868
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3987..4007)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3992..4321
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4172..4307
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4234..4253
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4369..4416
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4435..4809
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4939..5060
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4976..4995)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5030..5049
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5109..5125)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5109..5125)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5122..5144)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5133..5149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5157..5163,5164..5181,5182..5187))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5202..5223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5340..5357)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5511..6099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5591..5610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6270..7061,7062..7130))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6893..6912
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7131..7235)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7310..7329)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7501..7880
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7882..8080
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8035..8055
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8098..8278
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8325..8450
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8943..9176
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9361..9405
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9703..9820
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 acagggacag cagagatcca gtttggttaa ttaacccgtg tcggctccag atctggcctc
       61 cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt
      121 cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc
      181 gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg
      241 acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt
      301 agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat
      361 ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt
      421 tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg
      481 ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg
      541 ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa
      601 aatggcggct gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt
      661 tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa
      721 tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg
      781 aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc
      841 ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg
      901 tcacccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag
      961 gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag
     1021 gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta
     1081 tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg
     1141 agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt
     1201 aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg
     1261 ttagacgaag cttgggcccg ggaattaatt cgctagcacc atggaacaga aactgatctc
     1321 tgaagaagac ctggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg
     1381 ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg
     1441 ggtgtccgta actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga
     1501 catccatgtc atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa
     1561 aatttttaag gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg
     1621 cacactggta atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga
     1681 aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa
     1741 caaaattatc gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat
     1801 caacggagtg accggctggc ggctgtgcga acgcattctg gcgtgaaagc ttggtaccga
     1861 gctcggatcc actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg
     1921 ctcgagtcta gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     1981 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     2041 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     2101 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     2161 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     2221 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     2281 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     2341 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     2401 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     2461 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     2521 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg
     2581 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     2641 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     2701 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     2761 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     2821 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     2881 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
     2941 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
     3001 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
     3061 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     3121 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     3181 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     3241 ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     3301 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3361 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3421 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     3481 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     3541 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     3601 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     3661 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     3721 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     3781 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     3841 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     3901 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     3961 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     4021 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     4081 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4141 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4201 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4261 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     4321 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     4381 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     4441 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     4501 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     4561 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     4621 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     4681 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     4741 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     4801 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     4861 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     4921 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     4981 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5041 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     5101 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     5161 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     5221 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     5281 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5341 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5401 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5461 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5521 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5581 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5641 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5701 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5761 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5821 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5881 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5941 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6001 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6061 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6121 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6181 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6241 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6301 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6361 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6421 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6481 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6541 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6601 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6661 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6721 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6781 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6841 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     6901 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6961 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7021 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7081 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7141 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7201 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7261 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     7321 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     7381 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     7441 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     7501 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     7561 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     7621 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     7681 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     7741 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     7801 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     7861 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     7921 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     7981 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     8041 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
     8101 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8161 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8221 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8281 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     8341 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     8401 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     8461 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     8521 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     8581 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     8641 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     8701 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     8761 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     8821 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     8881 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     8941 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     9001 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     9061 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     9121 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     9181 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     9241 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     9301 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     9361 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     9421 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     9481 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     9541 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     9601 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     9661 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     9721 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     9781 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
//
